New Technologies in Transfusion Medicine

Comparison of conventional tube test with diamed gel microcolumn assay for anti-D titration

“…Anti‐D titration can be performed in tubes, microplates or using microcolumn tests. Three types of microcolumn tests have been developed since Lapierre's introduction of a new test involving the use of microtubes: gel microcolumn agglutination, affinity columns and glass microcapsules (Lapierre et al ., 1990; Walker, 1997).…”

The value of automated gel column agglutination technology in the identification of true inherited D blood types in massively transfused patients

“…Over the past 20 years, three automated methods (microwell, gel, and solid phase) have emerged and show no loss in accuracy or safety as compared to conventional tube-based methods. 3 Each technique of determining antigen status has its strengths and weaknesses. Conventional tube-based testing has been utilized for decades and is accepted as the standard.…”

“…We confirm that the gel column agglutination method is superior relative to other commonly used methods in the detection and following of mixed-field reaction patterns. 3,9,[11][12][13][14] While our case series reports the use of gel column agglutination for mixed-field identification in massively transfused patients, it has also been reported as useful in other settings such as monitoring hematopoeitic stem cell transplant patients and detecting chimerism in patients. [11][12][13][14] As confirmed by our comparative study, the gel column agglutination method in its automated format can reliably identify mixed-field reactions to at least a 10% limit of detection.…”

“…These automated techniques are replacing manual tube-based methods in civilian and military hospitals for several reasons including decreased personnel requirements, increased sensitivity and accuracy, and the ability to interface with hospital computer systems thus eliminating manual entry-associated clerical error. Previous articles have elicited advantages of both solid [3][4][5][6][7][8] and gel 3,[9][10][11] technologies compared to tube-based serology testing, but little mention has been given to the area of mixed-field detection with automated technologies. 3,9,10,[12][13][14][15] Gel column agglutination has unique advantages in detecting mixed-field agglutination patterns.…”

Evaluation of BioVue Column Agglutination Technology for quality control purpose of therapeutic anti-Rh immunoglobulin preparations

“…Three types of microcolumn tests have been developed: BioVue Column Agglutination Technology, affinity columns and glass microcapsules (Lapierre et al, 1990;Walker, 1997). The BioVue Column Agglutination Technology assay has been widely used in blood banking in Europe and America since 1991 for direct and indirect antiglobulin tests, ABO/Rh typing, red blood cell phenotyping, detecting unexpected antibodies, and for other applications (Lapierre et al, 1990).…”