New telomere formation after developmentally regulated chromosomal breakage during the process of chromatin diminution in ascaris lumbricoides

Müller, Fritz; Wicky, Chantal; Spicher, Albert; Tobler, Heinz

doi:10.1016/0092-8674(91)90076-b

Cited by 125 publications

(97 citation statements)

References 16 publications

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“…We have shown previously that a single cloned somatic telomere, pTel1, is created de novo during the elimination process by chromosomal breakage and addition of several kb of the telomeric repeat TTAGGC [1,6,7]. To assess whether the events observed at pTel1 reflect a general mechanism by which most if not all somatic telomeres are generated, we have analysed additional A. suum somatic telomeres.…”

Section: A Suum Somatic Telomeres Are Newly Created During Chromatinmentioning

confidence: 99%

“…With a 10-40% sucrose gradient [10] 7-10 kb long DNA fragments were selected and cloned in the EcoRI site of a ZapII bacteriophage (Stratagene). The unamplified library was screened with an A. suum telomeric (TTAGGC) 27 probe [6]. The library yielded several hundred positive clones of which some were randomly selected and isolated.…”

Section: Somatic a Suum End Librarymentioning

confidence: 99%

“…By gel extraction, fragments of 6-10 kb were isolated and cloned into a ZapII vector digested with EcoRI (Stratagene). The amplified library was then screened with a random primed DIG-labeled A. suum telomeric (TTAGGC) 27 probe [6]. Bluescript SK (−) plasmids containing the telomeric inserts were excised by following the Stratagene protocol.…”

Section: Somatic P Univalens Telomere Librarymentioning

confidence: 99%

“…Previously, we have cloned and analysed one somatic telomere (pTel1) from A. suum [4][5][6]. We found that it was newly formed during the process of chromatin diminution upon chromosomal breakage and addition of 4-6 kb of nematode telomeric repeats (TTAGGC) n to the chromosomal breakage sites [7].…”

Section: Introductionmentioning

confidence: 99%

“…Telomeric sequences do not exist at or near the breakage sites on the germ line chromosome and therefore are added de novo, probably by a developmentally regulated telomerase activity [7]. Interestingly, formation of pTel1 does not take place at a single chromosomal locus, but can occur at many different sites within a specific, several kb long chromosomal region, referred to as chromosomal breakage region 1 (CBR1) [6]. Telomeres are not only attached to the broken ends of the retained chromosomal portions in the pre-somatic cells, where they are most likely required for the maintenance of the chromosomal integrity, but also to the eliminated part of the chromosomes, which later on become degraded in the cytoplasm [5].…”

Section: Introductionmentioning

confidence: 99%

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Chromatin diminution leads to rapid evolutionary changes in the organization of the germ line genomes of the parasitic nematodes A. suum and P. univalens

Bachmann-Waldmann

Jentsch

Tobler

et al. 2004

Molecular and Biochemical Parasitology

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Section: A Suum Somatic Telomeres Are Newly Created During Chromatinmentioning

confidence: 99%

Section: Somatic a Suum End Librarymentioning

confidence: 99%

Section: Somatic P Univalens Telomere Librarymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chromatin diminution leads to rapid evolutionary changes in the organization of the germ line genomes of the parasitic nematodes A. suum and P. univalens

Bachmann-Waldmann

Jentsch

Tobler

et al. 2004

Molecular and Biochemical Parasitology

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Chromatin Diminution

Streit

Davis

2016

Encyclopedia of Life Sciences

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Chromatin diminution is defined as chromosomal fragmentation, followed by the elimination of part of the chromosome during mitosis. The process was first observed in early cleavage divisions of the parasitic nematode Parascaris equorum embryo by Boveri in 1887. It was later found to also occur in some other parasitic nematodes, as well as a number of unicellular and metazoan species in diverse taxonomic groups. While chromatin diminution occurs in diverse higher order taxa, within individual taxa, it occurs only in a rather small number of species. The process appears to play different biological roles in different organisms, may use different mechanisms and is likely to have arisen multiple times during evolution. Key Concepts The term ‘chromatin diminution’ describes the programmed elimination of parts of chromosomes during mitotic divisions and is distinguished from chromosome elimination, during which entire chromosomes are eliminated. Chromatin diminution, like chromosome elimination, can affect both copies of a chromosome leading to the complete removal of the corresponding genetic information. Alternatively, chromatin diminution or chromosome elimination can affect only one of the two homologous chromosomes leading to a reduction in gene dose. In other systems such a state where in one cell different regions of the genome are present at different copy numbers can be achieved by differential amplification of various regions of the genome. If chromatin diminution occurs only in some of the cells of an individual, it leads to a deviation from the dogma that all cells in an organism contain equal genetic information. In most known cases, chromatin diminution creates a difference between somatic and germ line cells or between the sexes. Chromatin diminution is a mechanism to silence genes (throwing them away approach to gene regulation) or reduce gene dosage. Chromatin diminution may also serve to reduce the burden of ‘selfish DNA’ in somatic cells while maintaining it in the gem line. Chromatin diminution has arisen independently multiple times during evolution (convergent evolution) and its mechanisms likely differ.

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A Modular Engineered DNA Nanodevice for Precise Profiling of Telomerase RNA Location and Activity

Zhang,

Lv,

Zhou

et al. 2024

Advanced Science

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Increased telomerase activity has been considered as a conspicuous sign of human cancers. The catalytic cores of telomerase involve a reverse transcriptase and the human telomerase RNA (hTR). However, current detection of telomerase is largely limited to its activity at the tissue and single‐cell levels. To reveal the precise distribution of subcellular hTR and telomerase activity, here a modular engineered DNA nanodevice (DNA‐ND) is designed capable of imaging hTR and telomerase activity in cytoplasm and nucleus, enabling colocalization analysis. DNA‐ND is a modular DNA complex comprising hTR and telomerase activity detection modules, which respectively sense intercellular hTR and telomerase activity via target‐sensitive allosteric transition of DNA switches, actuating orthogonal activation of fluorescence signals to achieve in situ co‐imaging of hTR and telomerase activity. By integrating DNA‐ND with specific localized signals, the DNA‐ND based precise profiling of colocalization of hTR and telomerase activity in different cell lines as well as their dynamic changes during pharmacological interventions is demonstrated. Notably, the results suggest that the locations of hTR and telomerase activity are not exactly overlapped, indicating the influence of intracellular environment on the binding of hTR to telomerase.

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New telomere formation after developmentally regulated chromosomal breakage during the process of chromatin diminution in ascaris lumbricoides

Cited by 125 publications

References 16 publications

Chromatin diminution leads to rapid evolutionary changes in the organization of the germ line genomes of the parasitic nematodes A. suum and P. univalens

Chromatin diminution leads to rapid evolutionary changes in the organization of the germ line genomes of the parasitic nematodes A. suum and P. univalens

Chromatin Diminution

A Modular Engineered DNA Nanodevice for Precise Profiling of Telomerase RNA Location and Activity

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