New terpenoids in cultivated and wild chamomile (in vivo and in vitro)

Szöke, Éva; Máday, E.; Tyihák, E.; Кузовкина, И. Н.; Lemberkovics, Éva

doi:10.1016/j.jchromb.2003.09.038

Cited by 46 publications

(35 citation statements)

References 17 publications

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“…The principal chemical components of the flowers include several phenolic compounds, primarily the flavonoids apigenin, quercetin, patuletin, luteolin and their glucosides. The main components of the essential oil extracted from the flowers are the terpenoids alpha-bisabolol and its oxides and azulenes, including chamazulene (5)(6)(7)(8)(9). Anti-inflammatory, antimicrobial and antioxidant medications are good choices for the treatment of wounds (10).…”

Section: Introductionmentioning

confidence: 99%

Effects of Chamomilla recutita (L.) on oral wound healing in rats

Duarte¹,

Quirino²,

Patrocínio³

et al. 2011

Med Oral

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Objectives. The purpose of this study was to evaluate the effect of Chamomilla recutita on the healing of ulcers in rats. Study Design. A 5-mm wound was inflicted on the tongue of 36 rats. Treatment group animals were treated topically with 0.04 mL/day of chamomile ointment, whereas control group animals were not treated. Animals were sacrificed after 3, 7 or 10 days. Semi-quantitative analysis of the degree of inflammation, fibroblast count and wound size was performed, as well as histometric analysis of re-epithelialization and percentage of collagen fibers of the lesion. Results. Animals treated with chamomile showed the best results regarding epithelialization and percentage of collagen fibers after 10 days. As expected, time had a statistically significant effect (p< 0.05) on fibroblast count, epithelialization, inflammation and wound size; animals sacrificed at 3 days showed the worst results. Conclusions. Chamomile stimulated re-epithelialization and the formation of collagen fibers after 10 days of treatment; it did not, however, influence inflammation or fibroblast count.

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Section: Introductionmentioning

confidence: 99%

Effects of Chamomilla recutita (L.) on oral wound healing in rats

Duarte¹,

Quirino²,

Patrocínio³

et al. 2011

Med Oral

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“…In this site, o-cymene (17 6%) and -thujene (13 6%) were the major constituents, while -bisabolol occurred in the lowest mean content (0.7-39%). In other sites, -bisabolol, which features an important antiinflammatory activity, 13 revealed a higher mean content (46 17%) (p < 0.05). All essential oil components, with the exception of n-nonanal, ar-curcumene, and other constituents (Table 1), showed interactions between populations and sampling sites.…”

Section: Resultsmentioning

confidence: 97%

Infraspecific variability in the essential oil composition of Lychnophora ericoides

Lyra¹,

Vieira²,

Oliveira³

et al. 2008

J. Braz. Chem. Soc.

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A variação na composição química do óleo essencial das folhas em três populações silvestres de Lychnophora ericoides, com ou sem odor, indicou a presença de dois grupos de óleos em relação ao odor das amostras. O primeiro incluiu amostras de odor aromático contendo altas percentagens de -bisabolol (34 23%) e o-cimeno (8,4 6,9%). No grupo II, as amostras não apresentaram qualquer odor e os constituintes majoritários foram o óxido de cariofileno (11 9%) e o -elemeno (5,7 6,9%). Em todas as populações estudadas co-existem os dois tipos de óleos, embora a maioria das amostras aromáticas provenientes do Parque Estadual da Serra de Caldas Novas se diferenciem quimicamente daquelas do Parque Nacional de Brasília e de Santo Antônio do Descoberto. As variações químicas sugerem claramente, além da influência geográfica, diferenças genéticas entre indivíduos nas populações. O alto conteúdo de -bisabolol suporta o uso medicinal folclórico de arnica como antiinflamatório.Variations in the composition of the leaf essential oils of wild Lychnophora ericoides, with and without scent, which were grown in three sampling sites, were examined by GC-MS. Results were submitted to principal component and cluster analysis which allowed for two groups of essential oils to be distinguished with regard to scent: cluster I with specimens exhibiting an aromatic scent and containing a high percentage of -bisabolol (34 23%) and o-cymene (8.4 6.9%); cluster II with specimens without any scent and characterised by a high percentage of caryophyllene oxide (11 9%) and -elemene (5.7 6.9%). The two types of oil coexist in all the populations under study, although patterns of aromatic samples deriving from Caldas Novas' State Park reveal chemical differences in relation to the samples from Brasília's National Park and Santo Antônio do Descoberto. Such chemical variations clearly suggest, apart from a geographical influence, genetic differences between individuals in the populations. The high content of -bisabolol supports the folk medicinal use of arnica as anti-inflammatory.Keywords: Lychnophora ericoides, Asteraceae, essential oils, chemical variability IntroductionThe genus Lychnophora Mart. (Asteraceae) consists of approximately 34 species, all of which grow in restricted regions of central Brazilian Cerrado at altitudes ranging from 950 to 1800 m above sea level in sandy sites, usually inside woodland and scrubs.1-3 L. ericoides Mart. is the most popular species, whose leaves are frequently aromatic and commercialised in local markets as analgesic and anti-inflammatory agents. 4,5 Its characteristic constituents are sesquiterpene lactones showing antiproliferative activity, 6-8 as well as flavonoids with anti-inflammatory effects, 9 triterpenes, 7,10 lignans, and caffeoylquinic acids with analgesic activity. 4,11 In the first phytochemical study of the essential oil of L. ericoides we identify a high degree of variability in the quantity and composition of leaf essential oils from wild populations with and without scent, each one growing on dif...

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“…The identification of the compounds was carried out by comparing retention times, retention indices and recorded spectra with data of authentic standards. Mass spectral and retention data known from the literature [8,[33][34][35] and the NIST 05 library were also consulted. The temperature programmed retention indices (I T ) were calculated according to the equation given by Van den Dool and Kratz [36].…”

Section: Apparatus and Gc-ms Conditionsmentioning

confidence: 99%

“…The beneficial effects are related to different classes of therapeutically interesting ingredients such as essential oil components, flavonoids, and coumarin derivatives [1, 5, and 6]. The chamomile essential oils, which are of the greatest importance among the bioactive constituents [7], consist of many active compounds such as (-)-alpha-bisabolol, chamazulene (artefact of matricine by steam distillation), (-)-alpha-bisabolol oxide A and B, (-)-alpha-bisabolone oxide A, spiroethers (cis-and trans-en-yn-dicycloether), and sesquiterpenes [8]. The chamomile volatile oils showed antimicrobial activity against certain species of bacteria, fungi and viruses in vitro [9], however, Gram-positive bacteria were found more susceptible than Gram-negative ones [10].…”

Section: Introductionmentioning

confidence: 99%

Separation and Identification of Antibacterial Chamomile Components Using OPLC, Bioautography and GC-MS

Móricz¹,

Szarka²,

Ott³

et al. 2012

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Components of 50% ethanolic chamomile (Matricaria recutica L.) flower extract, previously found antibacterial in a TLC-bioautographic study, were separated and isolated by the use of on-line OPLC, which consisted of an OPLC 50 BS system, an on-line coupled flow-through UV detector, and a manual fraction collector. The collected peaks were investigated by GC-MS analysis and by TLC re-chromatography with subsequent visualization, performed after use of the vanillin-sulphuric acid reagent, or under UV illumination, or applying bioautographic detection. The main compounds of the collected 11 fractions were identified by GC-MS. The results showed that the antibacterial effect of 50% ethanolic extract of chamomile is ascribable to cis-, trans-spiroethers, and the coumarins like herniarin and umbelliferone. Separation and identification of antibacterial chamomile components 3 INTRODUCTIONThere is an increased demand to discover new, effective, easy-to-obtain natural medicinal substances. Chamomile (Matricaria recutica L.) is a very familiar and widely used herb in traditional folk medicine. The flower is a common ingredient of herbal teas because it contains substances which have anti-inflammatory, sedative, analgesic, antispasmodic, antioxidant and antimicrobial properties [1][2][3][4]. The beneficial effects are related to different classes of therapeutically interesting ingredients such as essential oil components, flavonoids, and coumarin derivatives [1, 5, and 6]. The chamomile essential oils, which are of the greatest importance among the bioactive constituents [7], consist of many active compounds such as (-)-alpha-bisabolol, chamazulene (artefact of matricine by steam distillation), (-)-alpha-bisabolol oxide A and B, (-)-alpha-bisabolone oxide A, spiroethers (cis-and trans-en-yn-dicycloether), and sesquiterpenes [8]. The chamomile volatile oils showed antimicrobial activity against certain species of bacteria, fungi and viruses in vitro [9], however, Gram-positive bacteria were found more susceptible than Gram-negative ones [10]. The chamomile oils were effective e.g. [17,18]. Herniarin, the main coumarin component in chamomile, is a more effective antimicrobial agent than umbelliferone, which may be realized by its demethylation [19]. Umbelliferone has been described as a stress metabolite in M. recutita. The amount of umbelliferone characteristically, while that of herniarin mildly rose in the plant after biotic and abiotic stress influences [6,20]. It was also observed that chamomile oil component (Z)-spiroether was lowered after abiotic stress, but increased in the case of biotic stress [6]. Searching for bioactive natural products requires appropriate bioassays, focused on the desired activity (e.g. antifungal, antibacterial). Layer chromatography is an ideal technical solution for the screening of drug ingredients, because of high-throughput, low cost, easy maintenance and selectivity of detection reagents [21]. Direct bioautography [22][23][24][25][26], linking the separation with biological det...

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New terpenoids in cultivated and wild chamomile (in vivo and in vitro)

Cited by 46 publications

References 17 publications

Effects of Chamomilla recutita (L.) on oral wound healing in rats

Effects of Chamomilla recutita (L.) on oral wound healing in rats

Infraspecific variability in the essential oil composition of Lychnophora ericoides

Separation and Identification of Antibacterial Chamomile Components Using OPLC, Bioautography and GC-MS

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