New tool for diseases mechanism studies: Endoplasmic reticulum-targeted fluorescent probes

Tang, Qiannan; Li, Hening; Hu, Haoliang; Chen, Linxi

doi:10.1016/j.dyepig.2023.111634

Cited by 8 publications

(2 citation statements)

References 184 publications

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“…By virtue of NIR emission and stable optical and chemical properties, cyano isophorone fluorophore was selected as the probe skeleton and signal reporter (Scheme ). − Subsequently, a dipeptide glycylprolyl (Gly-Pro, consisting of proline and alanine), a known DPP4 responsive unit, − was modified on the amino group of dye skeleton to receive probe NEDP . The intrinsically nonfluorescent probe NEDP can be sensitively catalyzed and degraded by DPP4 to release the strongly fluorescent dye NEDD in vitro and in vivo , which brought a remarkable turn-on NIR fluorescence emission (673 nm).…”

Section: Resultsmentioning

confidence: 99%

Fabrication of a Near-Infrared-Emissive Probe for Detecting Dipeptidyl Peptidase 4 in the Liver of Diabetic Mice and Clinical Serum

Liu,

Zeng,

Zhang

et al. 2024

Anal. Chem.

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Dipeptidyl peptidase 4 (DPP4) plays a key role in glucose metabolism, which has been a close target for diabetes pathology and treatment. It is significant for the evaluation of cellular DPP4 activity in various biological systems. Fluorescence imaging technology is currently a popular method for detecting enzymes in living cells due to its advantages of high selectivity, high sensitivity, high spatiotemporal resolution, and real-time visualization. Herein, a near-infrared (NIR)-emissive probe NEDP with a large Stokes shift (153 nm) was developed for the assay of DPP4 activity. Upon addition of DPP4, NEDP can emit a significant turn-on NIR fluorescence signal (673 nm) with high sensitivity and specificity. Moreover, NEDP can successfully be used for imaging of intracellular DPP4, confirming the regulation of DPP4 expression in hyperglucose and its treatment in living cells. Most importantly, NEDP can not only monitor the changes of DPP4 in vivo but also show that DPP4 in diabetes is mainly upregulated in the liver, and the level of DPP4 is positively correlated with the pathological damage of the liver. In addition, NEDP can identify the serum of diabetic patients from healthy people through the fluorescence response to DPP4. These results demonstrated that the designed probe NEDP provides a prospective visual tool to explore the relationship between DPP4 and diabetes and would be applied for detecting serum of diabetes in the clinic.

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Section: Resultsmentioning

confidence: 99%

Fabrication of a Near-Infrared-Emissive Probe for Detecting Dipeptidyl Peptidase 4 in the Liver of Diabetic Mice and Clinical Serum

Liu,

Zeng,

Zhang

et al. 2024

Anal. Chem.

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“…The compounds were designed with a pentafluorophenyl substituent known for its propensity to target the endoplasmic reticulum 27 and were characterized by a pyridinium group that shows preferential localization within mitochondria. 28 We thus examined the influence S C H E M E 1 Synthetic route for the preparation of 1 and 2.…”

Section: Cellular Imagingmentioning

confidence: 99%

Fluorescent styrenes for mitochondrial imaging and viscosity sensing

Singh,

Ghorpade,

Regar

et al. 2024

Photochem & Photobiology

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Fluorophores bearing cationic pendants, such as the pyridinium group, tend to preferentially accumulate in mitochondria, whereas those with pentafluorophenyl groups display a distinct affinity for the endoplasmic reticulum. In this study, we designed fluorophores incorporating pyridinium and pentafluorophenyl pendants and examined their impact on sub‐cellular localization. Remarkably, the fluorophores exhibited a notable propensity for the mitochondrial membrane. Furthermore, these fluorophores revealed dual functionality by facilitating the detection of viscosity changes within the sub‐cellular environment and serving as heavy‐atom‐free photosensitizers. With easy chemical tunability, wash‐free imaging, and a favorable signal‐to‐noise ratio, these fluorophores are valuable tools for imaging mitochondria and investigating their cellular processes.

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Fluorogenic Probes for Functional Imaging of Endoplasmic Reticulum‐Resident Proteins: from Molecular Engineering to Biomedical Applications

Fan,

Zhang,

Hao

et al. 2024

Adv Funct Materials

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Endoplasmic reticulum‐resident proteins (ERPs) are crucial for maintaining cellular homeostasis and are tightly associated with the onset and progression of various diseases. The emergence of fluorogenic probes responsive to ERPs has enabled scientists to in situ monitor the spatial distribution and function status of target ERPs in real‐time, which is very helpful for elucidating ERP‐related pathological mechanisms and developing innovative therapeutics. Over the past decade, a range of fluorogenic probes for sensing and imaging ERPs, including ion channels, oxidases, hydrolases, and transferases, have been devised. This review systematically summarizes the recent advances in molecular engineering and biomedical applications of ERP fluorogenic probes. Engineering strategies for constructing ERP‐activatable probes, along with their structural features, recognition fragments, and sensing performances, are discussed. Meanwhile, biomedical applications of these probes in biomarker analysis, disease treatment, multimodal imaging, and drug discovery are also highlighted. Finally, a forward‐looking perspective on the future directions and underlying challenges is explored. The insights and knowledge presented here will spur further innovation and technological breakthroughs in developing multifunctional ERP probes, advancing disease diagnosis, ER biology, and drug discovery.

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New tool for diseases mechanism studies: Endoplasmic reticulum-targeted fluorescent probes

Cited by 8 publications

References 184 publications

Fabrication of a Near-Infrared-Emissive Probe for Detecting Dipeptidyl Peptidase 4 in the Liver of Diabetic Mice and Clinical Serum

Fabrication of a Near-Infrared-Emissive Probe for Detecting Dipeptidyl Peptidase 4 in the Liver of Diabetic Mice and Clinical Serum

Fluorescent styrenes for mitochondrial imaging and viscosity sensing

Fluorogenic Probes for Functional Imaging of Endoplasmic Reticulum‐Resident Proteins: from Molecular Engineering to Biomedical Applications

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