New Trends in Impedimetric Biosensors for the Detection of Foodborne Pathogenic Bacteria

Wang, Yixian; Ye, Zunzhong; Ying, Yibin

doi:10.3390/s120303449

Cited by 231 publications

(124 citation statements)

References 96 publications

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“…Typically, these biosensors involve the use of specialized equipment such as a spectrophotometer, electrochemical cell or quartz crystal microbalance and hence cannot be easily implemented outside the laboratory. 20,[22][23][24] Thus, there exists a need for a simple and rapid method of pathogen detection that does not require specialized training or expensive equipment. 25 We chose Staphylococcus aureus, a gram-positive micro-organism, as a model pathogen for testing the detection capabilities of our gold nanostars.…”

Section: Introductionmentioning

confidence: 99%

Branching and size of CTAB-coated gold nanostars control the colorimetric detection of bacteria

et al. 2014

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COMMUNICATIONRapid detection of pathogenic bacteria is challenging because conventional methods require long incubation times. Nanoparticles have the potential to detect pathogens before they can cause an infection. Gold nanostars have recently been used for colorimetric biosensors but they typically require surface modification with antibodies or aptamers for cellular detection. Here, CTAB-coated gold nanostars have been used to rapidly (<5 min) detect infective doses of a model gram-positive pathogen Staphylococcus aureus by an instrument-free colorimetric method. Varying the amounts of gold nanoseed precursor and surfactant can tune the size and degree of branching of gold nanostars as studied here by transmission electron microscopy. The size and morphology of gold nanostars determine the degree and rate of color change in the presence of S. aureus. The optimal formulation achieved maximum color contrast in the presence of S. aureus and produced a selective response in comparison to polystyrene microparticles and liposomes. These gold nanostars were characterized using UV-Visible spectroscopy to monitor changes in their surface plasmon resonance peaks. The visual color change was also quantified over time by measuring the RGB components of the pixels in the digital images of gold nanostar solutions. CTAB-coated gold nanostars serve as a promising material for simple and rapid detection of pathogens. IntroductionGold nanostars are an interesting class of materials because of their excellent performance in colorimetric biosensors, [1][2][3][4] surface enhanced Raman spectroscopy (SERS), [5][6][7][8][9][10][11] imaging and therapy, 12,13 as well as recently in solar cell power conversion. 14 The optical and electrical characteristics of gold nanostars are governed by their size and degree of branching. 11,15 Hence, control over these parameters is essential and has previously been demonstrated using methods such as seed-free growth, 16 the use of poly(vinylpyrrolidine) (PVP), 11,17,18 and even surfactant-free synthesis, but a systematic study of seedmediated synthesis assisted by the surfactant, cetyltrimethylammonium bromide (CTAB) is lacking. The use of nanoseed precursor and surfactant offers the opportunity to control the size and degree of branching of the nanostars using these two simple parameters. The morphology of nanostars determines the peak of light absorption and hence the color of gold nanostars. The peak of absorption in gold nanoparticles changes with their aggregation state. The shift in this peak causes a drastic color change that is detectable by the naked eye and is ideal for application in a biosensor. Furthermore, nanoparticles have increased kinetics in solution when compared with their microparticle counterparts, suggesting that rapid detection may be feasible using a biosensor platform at the nano-scale. 19,20 Food poisoning continues to cause severe illness around the world and leads to hospitalization of unsuspecting patients. The concentration of pathogens necessary for successfully ...

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Section: Introductionmentioning

confidence: 99%

Branching and size of CTAB-coated gold nanostars control the colorimetric detection of bacteria

et al. 2014

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“…3a. The most common bioreceptor for bacteria detection is an antibody (in this case, the term "immunosensor" is often used); however, other options have recently been presented, such as nucleic acids, bacteriophages and lectins (Wang et al, 2012). When the target bacteria bind to the bioreceptor, the redox reaction of [Fe(CN) 6 ] 3−/4− with the WE is hindered and the electrochemical cell impedance changes.…”

Section: Impedance Biosensorsmentioning

confidence: 99%

“…Recently, other bioreceptors have been tested such as nucleic acids, bacteriophages and lectins (Wang et al, 2012). Bacteriophages, in particular, are viruses that recognize and bind to specific receptors on the target bacteria.…”

Section: Impedance Biosensorsmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

311

160

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Abstract. Electrical impedance spectroscopy (EIS), in which a sinusoidal test voltage or current is applied to the sample under test to measure its impedance over a suitable frequency range, is a powerful technique to investigate the electrical properties of a large variety of materials. In practice, the measured impedance spectra, usually fitted with an equivalent electrical model, represent an electrical fingerprint of the sample providing an insight into its properties and behavior. EIS is used in a broad range of applications as a quick and easily automated technique to characterize solid, liquid, semiliquid, organic as well as inorganic materials. This paper presents an updated review of EIS main implementations and applications.

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“…Polyclonal antibodies raised against specific bacterial strains are the most commonly used bioreceptors for whole bacterial cell detection, where the binding targets on the cell envelope are usually unknown. To increase the specificity and sensitivity of the sensor, isolated surface epitopes can be used to produce monoclonal antibodies (32,33).…”

Section: Biosensors For Whole Bacterial Cell Detectionmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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New Trends in Impedimetric Biosensors for the Detection of Foodborne Pathogenic Bacteria

Cited by 231 publications

References 96 publications

Branching and size of CTAB-coated gold nanostars control the colorimetric detection of bacteria

Branching and size of CTAB-coated gold nanostars control the colorimetric detection of bacteria

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Biosensors for Whole-Cell Bacterial Detection

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