New type of antibody-enzyme conjugate which specifically kills Candida albicans

Abstract

A new type of antibody-enzyme conjugate was made, and its possible application to Candida infection was studied. Both lactoperoxidase and xanthine oxidase were conjugated to specific antibody against Candida albicans. In vitro microbiocidal activity of the new antibody-enzyme conjugate, when incubated together with xanthine and a minute amount of halides, showed a remarkable level of candidacidal ability. When the new antibody-enzyme conjugate was given to Candida-infected mice, followed by injecting xanthine and a minute amount of halides, about 50% of these heavily infected mice survived, whereas all control

Candidacidal activity of myeloperoxidase: therapeutic influence of the enzyme in vivo

“…Okuda et al (8) have suggested that lactoperoxidase might be useful for the treatment of cancer and infectious disease by complexing the enzyme with antibody specific for the desired target. Using a murine model of renal candidiasis, they demonstrated a significant therapeutic effect of lactoperoxidase conjugated to anti-Candida antibody.…”