New Vesicle Formation upon Oleate Addition to Preformed Vesicles

Chungcharoenwattana, Suparpun; Ueno, Masaki

doi:10.1248/cpb.53.260

Cited by 16 publications

(11 citation statements)

References 10 publications

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“…Although the physicochemical properties of fatty acid vesicles have been studied to some extent in the last three decades, there are still many aspects that require more detailed studies, e.g. regarding a model explaining the matrix effect [47][48][49] and fatty acid vesicle reproduction [26 • ]. The permeability of fatty acid vesicles to various classes of molecules remains largely unexplored.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid vesicles

Morigaki

Walde

2007

Current Opinion in Colloid & Interface Science

281

279

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Section: Discussionmentioning

confidence: 99%

Fatty acid vesicles

Morigaki

Walde

2007

Current Opinion in Colloid & Interface Science

281

279

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“…Several studies (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27) addressed the mechanism underlying the matrix effect and demonstrated that the fatty acids that originate from the initial micellar solution must first interact with the existing vesicles. However, the precise mechanism remained obscure and various pathways have been proposed.…”

Section: Introductionmentioning

confidence: 99%

Self-Reproduction of Fatty Acid Vesicles: A Combined Experimental and Simulation Study

Markvoort

Pfleger

Staffhorst

et al. 2010

Biophysical Journal

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Dilution of a fatty acid micellar solution at basic pH toward neutrality results in spontaneous formation of vesicles with a broad size distribution. However, when vesicles of a defined size are present before dilution, the size distribution of the newly formed vesicles is strongly biased toward that of the seed vesicles. This so-called matrix effect is believed to be a key feature of early life. Here we reproduced this effect for oleate micelles and seed vesicles of either oleate or dioleoylphosphatidylcholine. Fluorescence measurements showed that the vesicle contents do not leak out during the replication process. We hypothesized that the matrix effect results from vesicle fission induced by an imbalance of material across both leaflets of the vesicle upon initial insertion of fatty acids into the outer leaflet of the seed vesicle. This was supported by experiments that showed a significant increase in vesicle size when the equilibration of oleate over both leaflets was enhanced by either slowing down the rate of fatty acid addition or increasing the rate of fatty acid transbilayer movement. Coarse-grained molecular-dynamics simulations showed excellent agreement with the experimental results and provided further mechanistic details of the replication process.

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“…Similarly polymeric molecules, exhibit vesicles in aqueous/organic phase with the aid of various physical and environmental factors. The mediating and the environmental factors for the formation of vesicles are identified as: pH [5][6][7][8][9]; temperature [9][10][11][12]; chemical composition [7,10,[13][14][15][16][17][18][19][20][21][22]; surface-active compounds [11,12,14,19,20,[23][24][25][26][27][28][29][30][31][32][33][34][35][36] and finally, the agitation conditions [18].…”

Section: Introductionmentioning

confidence: 99%