New virus of the family Flaviviridae detected in lumpfish (Cyclopterus lumpus)

Skoge, Renate Hvidsten; Brattespe, Jarle; Økland, Arnfinn Lodden; Plarre, Heidrun; Nylund, Are

doi:10.1007/s00705-017-3643-3

Cited by 64 publications

(42 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the flaviviruses presented here encoded proteins on two discrete open reading frames, a number of transmembrane domains would not be produced between the predicted NS1 and the NS2 protein region. A similar genome structure was observed in Cyclopterus lumpus virus (CLuV), in which the production of a complete polyprotein is predicted to depend on a programmed Ϫ1 ribosomal frameshift (Ϫ1 PRF) on a "slippery" heptanucleotide sequence proximal to the stop codon (19). Programmed Ϫ1 ribosomal frameshifting has been documented to be used by many flaviviruses for the synthesis of additional proteins (45)(46)(47); however, only in CLuV has a Ϫ1 PRF been predicted for the production of a complete polyprotein.…”

Section: Resultsmentioning

confidence: 66%

“…As a result of these efforts, flaviviruses have been identified outside terrestrial vertebrates and invertebrates for the first time, in two groups of fish. The first of these marine vertebrate flaviviruses, Cyclopterus lumpus virus (CLuV), was identified in tissues of diseased lumpfish (Cyclopterus lumpus) (19). The second marine vertebrate flavivirus is Wenzhou shark flavivirus, identified in a metagenomic analysis of the Pacific spadenose shark, Scoliodon macrorhynchos (17).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Discovery of Novel Crustacean and Cephalopod Flaviviruses: Insights into the Evolution and Circulation of Flaviviruses between Marine Invertebrate and Vertebrate Hosts

Parry

Asgari

2019

J Virol

View full text Add to dashboard Cite

Most described flaviviruses (family Flaviviridae) are disease-causing pathogens of vertebrates maintained in zoonotic cycles between mosquitoes or ticks and vertebrate hosts. Poor sampling of flaviviruses outside vector-borne flaviviruses such as Zika virus and dengue virus has presented a narrow understanding of flavivirus diversity and evolution. In this study, we discovered three crustacean flaviviruses (Gammarus chevreuxi flavivirus, Gammarus pulex flavivirus, and Crangon crangon flavivirus) and two cephalopod flaviviruses (Southern Pygmy squid flavivirus and Firefly squid flavivirus). Bayesian and maximum likelihood phylogenetic methods demonstrate that crustacean flaviviruses form a well-supported clade and share a more closely related ancestor with terrestrial vector-borne flaviviruses than with classical insect-specific flaviviruses. In addition, we identify variants of Wenzhou shark flavivirus in multiple gazami crab (Portunus trituberculatus) populations, with active replication supported by evidence of an active RNA interference response. This suggests that Wenzhou shark flavivirus moves horizontally between sharks and gazami crabs in ocean ecosystems. Analyses of the mono-and dinucleotide composition of marine flaviviruses compared to that of flaviviruses with known host status suggest that some marine flaviviruses share a nucleotide bias similar to that of vector-borne flaviviruses. Furthermore, we identify crustacean flavivirus endogenous viral elements that are closely related to elements of terrestrial vector-borne flaviviruses. Taken together, these data provide evidence of flaviviruses circulating between marine vertebrates and invertebrates, expand our understanding of flavivirus host range, and offer potential insights into the evolution and emergence of terrestrial vector-borne flaviviruses.IMPORTANCE Some flaviviruses are known to cause disease in vertebrates and are typically transmitted by blood-feeding arthropods such as ticks and mosquitoes. While an ever-increasing number of insect-specific flaviviruses have been described, we have a narrow understanding of flavivirus incidence and evolution. To expand this understanding, we discovered a number of novel flaviviruses that infect a range of crustaceans and cephalopod hosts. Phylogenetic analyses of these novel marine flaviviruses suggest that crustacean flaviviruses share a close ancestor to all terrestrial vector-borne flaviviruses, and squid flaviviruses are the most divergent of all known flaviviruses to date. Additionally, our results indicate horizontal transmission of a marine flavivirus between crabs and sharks. Taken together, these data suggest that flaviviruses move horizontally between invertebrates and vertebrates in ocean ecosystems. This study demonstrates that flavivirus invertebrate-vertebrate host associations have arisen in flaviviruses at least twice and may potentially provide insights into the emergence or origin of terrestrial vector-borne flaviviruses. FIG 2 Translation of a complete polyprotein depends on highly st...

show abstract

Section: Resultsmentioning

confidence: 66%

mentioning

confidence: 99%

Discovery of Novel Crustacean and Cephalopod Flaviviruses: Insights into the Evolution and Circulation of Flaviviruses between Marine Invertebrate and Vertebrate Hosts

Parry

Asgari

2019

J Virol

View full text Add to dashboard Cite

show abstract

“…A growing number of infectious agents have been isolated from lumpfish (reviewed by Powell et al. (Powell et al., )), ranging from known pathogens that were likely transmitted from other fish species, such as Piscirickettsia salmonis (Marcos‐Lopez et al., ), to novel agents such as Cyclopterus lumpus virus ( Flaviviridae ) (Skoge, Brattespe, Økland, Plarre, & Nylund, ), lumpfish rana virus ( Iridoviridae ) and a new subtype of viral haemorrhagic septicaemia virus ( Rhabdoviridae ) (unpublished data). Lumpfish have also been shown to be able to transmit Neoparamoeba perurans , the parasite causing amoebic gill disease, to Atlantic salmon (Haugland, Olsen, Rønneseth, & Andersen, ).…”

Section: Introductionmentioning

confidence: 99%

“…Inevitably, when a new fish species is introduced to aquaculture, new diseases and infectious agents emerge (Alarcon et al, 2016). A growing number of infectious agents have been isolated from lumpfish (reviewed by Powell et al (Powell et al, 2017)), ranging from known pathogens that were likely transmitted from other fish species, such as Piscirickettsia salmonis (Marcos-Lopez et al, 2017), to novel agents such as Cyclopterus lumpus virus (Flaviviridae) (Skoge, Brattespe, Økland, Plarre, & Nylund, 2018), lumpfish rana virus (Iridoviridae) and a new subtype of viral haemorrhagic septicaemia virus (Rhabdoviridae) (unpublished data).…”

Section: Introductionmentioning

confidence: 99%

Moritella viscosa in lumpfish (Cyclopterus lumpus) and Atlantic salmon (Salmo salar)

Einarsdottir

Sigurdardottir

Björnsdottir

et al. 2018

Journal of Fish Diseases

View full text Add to dashboard Cite

Winter ulcer disease, caused by Moritella viscosa, is a significant problem in cold water salmonid farming, although the bacterium can infect and cause disease in a number of other fish species, such as lumpfish (Cyclopterus lumpus). Lumpfish are used as cleaner fish, to eat sea lice from Atlantic salmon (Salmo salar) in sea pens. It remains to be established whether M. viscosa can be transmitted between the fish species. In this study, we examined whether a salmon isolate of M. viscosa could infect and cause disease in lumpfish. We further examined whether a lumpfish isolate of M. viscosa could infect and cause disease in salmon. Finally, we examined whether vaccination of salmon with a salmon isolate of M. viscosa conferred protection against a lumpfish isolate. The data indicate that while lumpfish appeared to be resistant to a salmon isolate of M. viscosa, the salmon could be infected with a lumpfish isolate of M. viscosa. Vaccination protected the salmon against the salmon isolate of M. viscosa but did not confer sufficient protection to prevent infection with the lumpfish isolate.

show abstract

“…New diseases and infectious agents inevitably emerge when new species are introduced to aquaculture, and the lumpfish appears to pose no exception to this rule. A range of different pathogens has been detected in wild and farmed lumpfish, including viruses, bacteria, parasites and fungi (Alarcón, Gulla, et al, 2016;Alarcón, Thoen, et al, 2016;Guðmundsdóttir et al, 2019;Hjeltnes, 2014;Johansen, 2013;Marcos-López, Donald, Stagg, & McCarthy, 2013;Poppe et al, 2012;Scholz et al, 2017;Scholz, Ruane, Marcos-Lopez, et al, 2018;Skoge, Brattespe, Økland, Plarre, & Nylund, 2018;Stagg et al, 2017;Treasurer & Birkbeck, 2018). Finally, the possible role of cleaner fish as subclinical carrier for viral disease has emerged (Rimstad Basic, Gulla, & Hjeltnes & Mortensen, 2017).…”

mentioning

confidence: 99%

Lumpfish (Cyclopterus lumpus, Linnaeus) is susceptible to viral nervous necrosis: Result of an experimental infection with different genotypes of Betanodavirus

Toffan

Salvador

Scholz³

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

In recent years, the use of cleaner fish for biological control of sea lice has increased considerably. Along with this, a number of infectious diseases have emerged. The aim of this study was to investigate the susceptibility of lumpfish (Cyclopterus lumpus) to Betanodavirus since it was detected in asymptomatic wild wrasses in Norway and Sweden. Three betanodaviruses were used to challenge lumpfish: one RGNNV genotype and two BFNNV genotypes. Fish were injected and monitored for 4 weeks. Brain samples from clinically affected specimens, from weekly randomly selected fish and survivors were subjected to molecular testing, viral isolation, histopathology and immunohistochemistry. Reduced survival was observed but was attributed to tail‐biting behaviour, since no nervous signs were observed throughout the study. Betanodavirus RNA was detected in all samples, additionally suggesting an active replication of the virus in the brain. Viral isolation confirmed molecular biology results and revealed a high viral titre in BFNNV‐infected groups associated with typical lesions in brains and eyes of survivor fish. We concluded that lumpfish are susceptible to Betanodavirus, as proven by the high viral titre and brain lesions detected, but further studies are necessary to understand if Betanodavirus can cause clinical disease in this species.

show abstract

New virus of the family Flaviviridae detected in lumpfish (Cyclopterus lumpus)

Cited by 64 publications

References 31 publications

Discovery of Novel Crustacean and Cephalopod Flaviviruses: Insights into the Evolution and Circulation of Flaviviruses between Marine Invertebrate and Vertebrate Hosts

Discovery of Novel Crustacean and Cephalopod Flaviviruses: Insights into the Evolution and Circulation of Flaviviruses between Marine Invertebrate and Vertebrate Hosts

Moritella viscosa in lumpfish (Cyclopterus lumpus) and Atlantic salmon (Salmo salar)

Lumpfish (Cyclopterus lumpus, Linnaeus) is susceptible to viral nervous necrosis: Result of an experimental infection with different genotypes of Betanodavirus

Contact Info

Product

Resources

About