New wide dynamic range assays for quantification of anti- Leishmania IgG2 and IgA antibodies in canine serum

Clinical leishmaniosis in a captive Eurasian otter (Lutra lutra) in Spain: a case report

“…In dogs, the serum total levels of anti- Leishmania IgG, IgG1 and IgG2 are elevated during the active phase of Leishmania infection, the IgG2 being the predominant subclass [ 12 , 13 ]. At this moment, elevated levels of anti- Leishmania IgG2 in serum were found, as previously reported in dogs with clinical leishmaniosis using TR-IFMA [ 6 ]. High levels of anti- Leishmania IgG were also detected in the serum from the sick otter by the commercial ELISA.…”

“…The presence of anti- Leishmania IgG2 antibodies was evaluated in the serum of the otter by a time-resolved immunofluorometric assay (TR-IFMA) performed as described by Cantos-Barreda et al [ 6 ]. In order to test that the TR-IFMA assay validated for dog serum can be applied to otter serum, a Western blot analysis was carried out.…”

Time-Resolved FRET-Based Immunosensor for the Ultrasensitive and Rapid Detection of Cd2+

“…Shen et al established time-resolved fluorescence microspheres (TRFMs) for the detection of hydrochlorothiazide, and the limit of detection (LOD) was 1/14 lower than the liquid chromatography-tandem mass spectrometry method. Besides, the TRF materials were also extensively used in medical diagnosis − and the detection of food contaminants, such as biotoxins, pathogenic microorganisms, pesticides, heavy metal ions, biomarkers, etc. − Recently, several researchers indicated that compared with ELISA, the sensitivity of the TRF immunosensor was greatly improved by 1–2 orders of magnitude and the measuring time was significantly reduced by more than 50%. , Moreover, TRF sensors can be effectively operated to increase the signal-to-background ratio with their special fluorescence properties. , …”