Newborn screening by matrix-assisted laser desorption/ionization mass spectrometry based on parylene-matrix chip

Kim, Jo Il; Noh, Joo Yoon; Kim, Mi-Ra; Park, Jong Min; Song, Hyun Woo; Kang, Min Jung; Pyun, Jae Chul

doi:10.1016/j.ab.2017.04.021

Cited by 11 publications

(12 citation statements)

References 22 publications

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“…MALDI-TOF analysis was conducted as previously described using a parylene-matrix chip. ,,− LPC 16:0 standard and serum extracts were dispensed onto the parylene-matrix chip arrays with 0.5 μL of sample per spot and quantified using a Microflex mass spectrometer (Bruker Daltonics) equipped with a nitrogen laser (337 nm). Mass spectra were obtained in the reflective positive mode with a first accelerating voltage of 20 kV and a second ion source voltage of 16 kV.…”

Section: Methodsmentioning

confidence: 99%

“…These results show that the parylene-matrix chip can effectively prevent the production of mass peaks from the fragmented matrix. By using this advantage, MALDI-TOF MS based on the parylene-matrix chip was used for the analysis of amino acids in a metabolite test for newborns, an antibiotics susceptibility test for carbapenems, − and penicillin detection in milk. , …”

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confidence: 99%

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MALDI-TOF Mass Spectrometry Based on Parylene-Matrix Chip for the Analysis of Lysophosphatidylcholine in Sepsis Patient Sera

et al. 2019

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In this work, medical diagnosis of sepsis was conducted via quantitative analysis of lysophosphatidylcholine 16:0 (LPC 16:0) by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry based on a parylene-matrix chip. In the first step, specific mass peaks for the diagnosis of sepsis were searched by comparing MALDI-TOF mass spectra of sepsis patient sera with healthy controls and pneumonia patient sera. Two mass peaks at m/z = 496.3 and 518.3 were chosen as those that are specifically different for sepsis sera to compare with healthy controls and pneumonia patient sera. These mass peaks were identified to be protonated and sodium adducts of LPC 16:0 by using tandem mass spectra (MS2 and MS3) of purely synthesized LPC 16:0 and extracted LPC 16:0 from a healthy control and a sepsis patient. In the next step, a standard curve for LPC 16:0 for the quantitative analysis of LPC 16:0 with MALDI-TOF MS based on the parylene-matrix chip was prepared, and the statistical correlation to the LC-MS analysis results was demonstrated by using the Bland–Altman test and Passing–Bablok regression. Finally, MALDI-TOF MS based on the parylene-matrix chip was used for the quantification of LPC 16:0 with sera from patients with severe sepsis and septic shock (n = 143), pneumonia patients (n = 12), and healthy sera (n = 31). The sensitivity and the selectivity of medical diagnosis of sepsis was estimated to be 97.9% and 95.5% by using MALDI-TOF MS based on the parylene-matrix chip, respectively.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

MALDI-TOF Mass Spectrometry Based on Parylene-Matrix Chip for the Analysis of Lysophosphatidylcholine in Sepsis Patient Sera

et al. 2019

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“…Depending on the position of the laser radiation, different amounts of analyte ions occur even for the crystals of the same concentration. Thus, the quantitative analysis of small molecules has been restricted by MALDI-TOF MS (Park et al 2019;Kim et al 2017;Kim et al 2020;Choi et al 2018;Noh et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Laser desorption/ionization mass spectrometry of L-thyroxine (T4) using combi-matrix of α-cyano-4-hydroxycinnamic acid (CHCA) and graphene

et al. 2022

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An optimal combi-matrix for MALDI-TOF mass spectrometry was presented for the analysis of L-thyroxine (T4) in human serum. For the selection of the optimal combi-matrix, several kinds of combi-matrices were prepared by mixing the conventional organic matrix of CHCA with nanomaterials, such as graphene, carbon nanotubes, nanoparticles of Pt and TiO2. In order to select the optimal combi-matrix, the absorption at the wavelength of laser radiation (337 nm) for the ionization of sample was estimated using UV–Vis spectrometry. And, the heat absorption properties of these combi-matrices were also analyzed using differential scanning calorimetry (DSC), such as onset temperature and fusion enthalpy. In the case of the combi-matrix of CHCA and graphene, the onset temperature and fusion enthalpy were observed to be lower than those of CHCA, which represented the enhanced transfer of heat to the analyte in comparison with CHCA. From the analysis of optical and thermal properties, the combi-matrix of CHCA and graphene was selected to be an optimal combination for the transfer of laser energy during MALDI-TOF mass spectrometry. The feasibility of the combi-matrix composed of CHCA and graphene was demonstrated for the analysis of T4 molecules using MALDI-TOF mass spectrometry. The combi-matrix of CHCA and graphene was estimated to have an improved limit of detection and a wider detection range in comparison with other kinds of combi-matrices. Finally, the MALDI-TOF MS results of T4 analysis using combi-matrix were statistically compared with those of the conventional immunoassay.

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“…After sample treatment, mass peaks of analytes could be clearly detected without mass peaks of fragmented organic matrix molecules at a low m/z range of less than 500. MALDI-TOF MS based on the parylene-matrix chip thus allowed for quantitative analysis of small molecules in various medical diagnoses with biomarkers of less than 500 Da, such as amino acids for neonatal metabolite analyses, 11 antibiotics in dairy products, 12,13,17 and carbapenemase assays. 14,15,18 In this study, the MALDI-TOF MS based on the parylenematrix chip was used to clinically diagnose intrahepatic cholangiocarcinoma (IHCC) and colorectal cancer (CRC) by detecting the small biomarkers, as shown in Figure 1a.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Recently, the parylene-matrix chip was developed to quantify small molecules of less than 1 kDa. − As shown in Figure b, the parylene-matrix chip was generated by (1) dropping and drying an organic matrix and (2) deposition of a thin parylene film with a thickness of less than 50 nm. Upon laser radiation of the parylene-matrix chip, no mass peaks were observed from the fragmented organic matrix molecules.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip

Park

Kim

Noh

et al. 2020

J. Am. Soc. Mass Spectrom.

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Recently, the parylene-matrix chip was developed for quantitative analysis of small molecules less than 1 kDa. In this study, MALDI-TOF MS based on the parylene-matrix chip was performed to clinically diagnose intrahepatic cholangiocarcinoma (IHCC) and colorectal cancer (CRC). The parylene-matrix chip was applied for the detection of small cancer biomarkers, including N-methyl-2-pyridone-5-carboxamide (2PY), glutamine, lysophosphatidylcholine (LPC) 16:0, and LPC 18:0. The feasibility of MALDI-TOF MS based on the parylene-matrix chip was confirmed via analysis of spot-to-spot and shot-to-shot reproducibility. Serum metabolite markers of IHCC, N-methyl-2-pyridone-5-carboxamide (2PY), and glutamine were quantified using MALDI-TOF MS based on the parylene-matrix chip. For clinical diagnosis of CRC, two water-insoluble (barely soluble) biomarkers, lysophosphatidylcholine (LPC) 16:0 and LPC 18:0, were quantified. Finally, glutamine and LPC 16:0 were simultaneously detected at a range of concentrations in sera from colon cancer patients using the parylene-matrix chip. Thus, this method yielded high-throughput detection of cancer biomarkers for the mixture samples of water-soluble analytes (2PY and glutamine) and water-insoluble analytes (LPC 16:0 and LPC 18:0).

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Newborn screening by matrix-assisted laser desorption/ionization mass spectrometry based on parylene-matrix chip

Cited by 11 publications

References 22 publications

MALDI-TOF Mass Spectrometry Based on Parylene-Matrix Chip for the Analysis of Lysophosphatidylcholine in Sepsis Patient Sera

MALDI-TOF Mass Spectrometry Based on Parylene-Matrix Chip for the Analysis of Lysophosphatidylcholine in Sepsis Patient Sera

Laser desorption/ionization mass spectrometry of L-thyroxine (T4) using combi-matrix of α-cyano-4-hydroxycinnamic acid (CHCA) and graphene

Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip

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