Newcastle disease virus fusion and haemagglutinin-neuraminidase gene motifs as markers for viral lineage

Gould, Allan R.; Hansson, Eric; Selleck, Kathleen; Kattenbelt, J. A.; MacKenzie, Margaret; Della-Porta, Antony

doi:10.1080/0307945031000121112

Cited by 43 publications

(30 citation statements)

References 40 publications

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“…Further phylogenetic analysis based on a 374-bp fragment of the F gene in class I strains of NDV demonstrated that NDV08-004 belongs to genotype 3, and is similar to strains obtained from live bird markets in Hong Kong in recent years [8]. The size of HN protein can be acted as markers for viral lineage [17,18]. Although the entire HN gene for NDV is 2002 or 2001 nt, but the size of HN protein is different because of differences in the stop codon's position as a result of point mutation.…”

Section: Discussionmentioning

confidence: 97%

Genomic characterization of the first class I Newcastle disease virus isolated from the mainland of China

et al. 2010

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The complete genomic sequence of Newcastle disease virus (NDV) strain NDV08-004, isolated from domestic ducks in China, was determined in this study. The genome is 15198 nucleotides (nt) in length, follows the "rule of six" and contains a 55-nt leader sequence at the 3' end and a 114-nt trailer sequence at the 5' end. Compared with the full genome sequences of Class II NDV strains, the NDV08-004 isolate has a 12-nt insertion (TGGGAGACGGGG) in the phosphoprotein gene between nucleotides 2381 and 2382 of the genome (numbered according to the genomic sequence of the La Sota strain, which consists of 15186 nt). Strain NDV08-004 has the motif (112)E-Q-Q-E-R-L(117) at the cleavage site of the fusion protein, which is typical of lentogenic NDV strains, and this is in agreement with the results of pathogenic tests based on the mean death time (MDT) and the intracerebral pathogenicity index (ICPI). Phylogenetic analysis based on the full genome revealed that all the NDV strains studied could be divided into two distinct clades, namely class I and class II, and the NDV08-004 isolate characterized in this study was grouped in class I. Further phylogenetic analysis based on a 374-bp fragment of the F gene in class I strains of NDV demonstrated that NDV08-004 belongs to genotype 3, and should be therefore similar to strains obtained from live bird markets in Hong Kong in recent years.

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Section: Discussionmentioning

confidence: 97%

Genomic characterization of the first class I Newcastle disease virus isolated from the mainland of China

et al. 2010

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“…The viruses that caused historical outbreaks of ND in Australia in the 1930s had no HN extension, and those associated with recent outbreaks of ND in Australia had 9-amino-acid extensions. Viruses associated with the acute respiratory disease 'late respiratory syndrome' (Hooper et al, 1999) had 9-or 14-amino-acid HN extensions and it was suggested that viruses with 7-or 11-amino-acid extensions might also be associated with respiratory disease (Gould et al, 2003). However, the authors noted that a number of viruses with fewer than 45-amino-acid extensions showed no involvement with respiratory disease.…”

Section: Discussionmentioning

confidence: 97%

“…Division of ND viruses into various genetic groupings has been based on nucleotide sequencing and phylogenetic analysis of a number of genes including the F, HN, M and P genes Seal, 1996;Lomniczi et al, 1998;Locke et al, 2000;Aldous et al, 2003;Gould et al, 2003). Sakaguchi and colleagues (1989) divided ND viruses into three broad lineages (A, B and C) based on sequence comparison and size of the HN glycoprotein.…”

Section: Discussionmentioning

confidence: 99%

“…Phylogenetic analyses were done on strain I-2 (data not shown) and grouped this virus with other Australian isolates as belonging to Group A (or Group I-7) using HN sequences Gould et al, 2001Gould et al, , 2003 or lineage 1 as defined by Aldous and colleagues (2003) or Genotype I (Lomniczi et al, 1998;Gould et al, 2003) using F gene sequence analyses.…”

Section: C-terminus Of the Hn Proteinmentioning

confidence: 99%

“…Molecular studies indicated that the virulent virus was derived from existing Australian strains of NDV (Gould et al, 2001(Gould et al, , 2003. Lentogenic strains of NDV have been endemic in Australia since at least 1966 and many lentogenic strains have been isolated (Simmons, 1967;Kim et al, 1978;Spradbrow et al, 1995).…”

Section: Introductionmentioning

confidence: 98%

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Deduced Amino Acid Sequences Surrounding the Fusion Glycoprotein Cleavage Site and of the Carboxyl-terminus of Haemagglutinin–Neuraminidase Protein of the Avirulent Thermostable Vaccine Strain I-2 of Newcastle disease virus

et al. 2006

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A single-tube RT-PCR technique generated a 387 bp or 300 bp cDNA amplicon covering the F(0) cleavage site or the carboxyl (C)-terminus of the HN gene, respectively, of Newcastle disease virus (NDV) strain I-2. Sequence analysis was used to deduce the amino acid sequences of the cleavage site of F protein and the C-terminus of HN protein, which were then compared with sequences for other NDV strains. The cleavage site of NDV strain I-2 had a sequence motif of (112) RKQGRLIG(119), consistent with an avirulent phenotype. Nucleotide sequencing and deduction of amino acids at the C-terminus of HN revealed that strain I-2 had a 7-amino-acid extension (VEILKDGVREARSSR. This differs from the virulent viruses that caused outbreaks of Newcastle disease in Australia in the 1930s and 1990s, which have HN extensions of 0 and 9 amino acids, respectively. Amino acid sequence analyses of the F and HN genes of strain I-2 confirmed its avirulent nature and its Australian origin.

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Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections

2013

Diseases of Poultry

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Newcastle disease virus fusion and haemagglutinin-neuraminidase gene motifs as markers for viral lineage

Cited by 43 publications

References 40 publications

Genomic characterization of the first class I Newcastle disease virus isolated from the mainland of China

Genomic characterization of the first class I Newcastle disease virus isolated from the mainland of China

Deduced Amino Acid Sequences Surrounding the Fusion Glycoprotein Cleavage Site and of the Carboxyl-terminus of Haemagglutinin–Neuraminidase Protein of the Avirulent Thermostable Vaccine Strain I-2 of Newcastle disease virus

Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections

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