Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis

Ravindra, P. V.; Tiwari, Ashok Kumar; Ratta, Barkha; Chaturvedi, Uttara; Palia, Sudesh Kumar; Chauhan, R.S.

doi:10.1016/j.virusres.2008.12.008

Cited by 60 publications

(39 citation statements)

References 34 publications

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“…The isolation rate was lower in avian embryos might be due to presence of maternal antibody in embryonated eggs. The growth of virus in BHK-21 cell line were confirmed by the presence of cytopathic effects (CPE) which are the characteristics of syncitia formation, rounding of cell and multinucleated giant cells which are typical for NDV which are close agreement with the findings of Gerganov (1978) and Ravindra et al ,(2009).…”

Section: Discussionsupporting

confidence: 87%

Development of BHK-21 Cell line Adapted Inactivated Newcastle Disease Vaccine

et al. 2014

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The present study was undertaken to isolate and characterize Newcastle disease virus (NDV) from the outbreak of layer farms for the development of BHK-21 cell adapted inactivated vaccine. A total of 6 dead birds were brought to the laboratory from the outbreak area from which 18 samples (trachea, liver and brain from each) were collected. Among them 3 samples were found positive for NDV through chicken embryo inoculation followed by HA test. The MDT, ICPI, IVPI of all isolates was 54.4, 1.56, and 2.20 respectively which revealed that the field isolates were velogenic. The isolated viruses were confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers. The isolated virus was used to infect the BHK-21 cell line. Later the BHK-21 cell adapted viruses were used to develop oil adjuvanted inactivated vaccine and were inoculated into chickens according to vaccination schedule. The MDA was very high (112±29.62) during BCRDV vaccination, which declined quickly (88±33.12). Before vaccination with experimental vaccine, the level of antibody titre (HI) was very low (9±4.65). After vaccination at 65 th day through IM, ELD50=109.7/ml with experimental vaccine, the highest HI titre in RDV vaccinated group was 160±59.25 whereas, experimental vaccinated group was 128±59.25, and control group was 7±4.65. ELISA antibody titres of all the groups were 2549.71 (RDV, LRI@0.5 ml/bird), 2450.37 (experimental@ 0.25ml/ bird), 2218.579 (experimental@ 0.50ml/bird), 2152.352 (experimental@1ml/bird) 1125.846 (control) respectively. The present study indicated that, BHK-21 cell adapted ND inactivated vaccine produced a satisfactory antibody titre along with conventional live RDV vaccine.

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Section: Discussionsupporting

confidence: 87%

Development of BHK-21 Cell line Adapted Inactivated Newcastle Disease Vaccine

et al. 2014

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“…So for successful isolation of NDV, it is necessary to avoid excessive bacterial contamination. The CPE characterized by increased granularity, rounding and vacuolation of infected cells followed by the formation of syncytia were typical of NDV (Ravindra et al, 2009;Mehrabanpour et al, 2010). A relatively large number of syncytia was found with sample C-67 and C-162.…”

Section: Resultsmentioning

confidence: 93%

Isolation and identification of Newcastle disease viruses from field outbreaks in chickens and pigeons

et al. 2013

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Eleven dead or sick birds submitted from farms in the year 2010 with a history of sudden death with respiratory and/or diarrhoeal signs were used for isolation and identification of Newcastle disease virus (NDV). All samples were subjected to routine necropsy. Pooled respiratory tissues were inoculated in embryonated chicken eggs and chicken embryo fibroblast (CEF) cell culture. The growth of NDV was confirmed by embryo mortality, cytopathic effects (CPE) in cell culture, haemagglutination (HA) and haemagglutination inhibition (HI) test. The presence of NDV was confirmed by reverse transcriptionpolymerase chain reaction (RT-PCR). At necropsy seven cases were tentatively diagnosed as Newcastle disease (ND). Out of seven ND-suspected samples, four yielded virus in both embryos and cell culture, while one was positive only in embryos, one only in cell culture and one sample was negative in both embryos and cell culture. RT-PCR successfully amplified a 766 bp fragment covering parts of Matrix and Fusion protein genes of NDV from the samples that were positive either in embryos or in cell culture. It is suggested that RT-PCR could be a rapid and sensitive tool for the detection of NDV.

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“…It has been proposed that the ratio of Bax/ Bcl-2 serves as a rheostat that dictates cell survival vs. death [24,34] . By real-time PCR it has been recently reported that in NDV-infected Vero cells, Bax/Bcl-2 ratio is increased [47] . In our study, by Western blotting analysis of cell lysates, we did not detect significant changes in the total levels of these two proteins before and after infection with the NDV strain AF2240, indicating that the ratio of Bax/Bcl-2 at the protein level remained constant.…”

Section: Discussionmentioning

confidence: 99%

Newcastle Disease Virus Infection Promotes Bax Redistribution to Mitochondria and Cell Death in HeLa Cells

Molouki¹,

Hsu

Jahanshiri³

et al. 2009

Intervirology

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Background/Aims: Newcastle disease virus (NDV) is an avian paramyxovirus that has gained a lot of interest in cancer viro-therapeutic applications because of its ability to selectively induce apoptosis in human cancer cells. However, the underlying mechanisms by which NDV induces apoptosis in human cancer cells are still not entirely understood. Methods: In this study we examined the effect of a Malaysian velogenic strain of NDV, known as AF2240, on some elements of the intrinsic pathway of apoptosis. Results: We show that NDV infection leads to conformational change of Bax protein. This is associated with the translocation of Bax from the cytoplasm to mitochondria and the release of cytochrome c into the cytoplasm. Interestingly, the level of Bcl-2 protein was not affected by NDV treatment. Conclusion: We have shown that Bax conformational change and subcellular distribution is involved in the intrinsic pathway of apoptosis induced by NDV.

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Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis

Cited by 60 publications

References 34 publications

Development of BHK-21 Cell line Adapted Inactivated Newcastle Disease Vaccine

Development of BHK-21 Cell line Adapted Inactivated Newcastle Disease Vaccine

Isolation and identification of Newcastle disease viruses from field outbreaks in chickens and pigeons

Newcastle Disease Virus Infection Promotes Bax Redistribution to Mitochondria and Cell Death in HeLa Cells

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