Newer HRT regimens

Sturdee, David W.

doi:10.1111/j.1471-0528.1997.tb10932.x

Cited by 12 publications

(2 citation statements)

References 68 publications

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“…Progestins are frequently prescribed to postmenopausal women in combination with an estrogen for treating osteoporosis (Prior 1990, Sturdee 1997. The estrogenic component in hormone replacement therapy (HRT) exerts a well-known positive effect on bone mass.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the progestin receptors in the human TE85 and murine MC3T3-E1 osteoblast-like cell lines

Gunnet¹,

Granger²,

Cryan³

et al. 1999

Journal of Endocrinology

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Progestins are believed to exert positive effects on bone density through receptors located in osteoblasts. In the present studies, the binding characteristics and regulation of the progestin receptors in two osteoblast-like cell lines were compared with those in human breast lines. Human TE85 and murine MC3T3-E1 osteoblast-like cells contain a single, high-affinity progestin binding site whose affinity and concentration are lower than in human breast cells. The osteoblastic progestin binding sites showed the expected steroid specificity and associated with the cell nuclei when occupied by ligand. The progestin receptors in osteoblastic cells also had sedimentation coefficients similar to those receptors in breast cells. The regulation of the progestin receptor in the osteoblast-like cells was explored by treating them with estradiol. In contrast to the large, rapid change seen in the breast cells, the progestin receptor levels in the MC3T3-E1 cells showed only a small, delayed up-regulation with estradiol treatment. The progestin receptor number in the TE85 cells was unaffected by estradiol. Down-regulation of the progestin receptors was explored by treating the cells with the progestin, norethindrone (NET). NET administration produced a rapid drop in progestin binding sites in the breast cells and a smaller, more gradual decline in MC3T3-E1 progestin binding. While the maximal decrease in receptor number occurred within 24 h in the breast cells, the receptor number was still continuing to fall after 72 h in the MC3T3-E1 cells. The data presented here demonstrate that both human and murine osteoblastlike cells contain a functional progestin receptor whose binding characteristics and regulation are similar, but not identical, to those receptors in other progestin target tissues such as the breast.

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Section: Introductionmentioning

confidence: 99%

Characterization of the progestin receptors in the human TE85 and murine MC3T3-E1 osteoblast-like cell lines

Gunnet¹,

Granger²,

Cryan³

et al. 1999

Journal of Endocrinology

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show abstract

“…2 This occurs despite the development of continuous combined regimes with the aim to induce amenorrhoea or long cycle HRT to limit the frequency of bleeding. 3 Menstrual bleeding is a phenomenon restricted to humans and sub-human primates, which in general have coiled blood vessels or spiral arterioles in their endometrium. Spiral arterioles undergo growth or angiogenesis throughout each menstrual cycle.…”

Section: Introductionmentioning

confidence: 99%