Newer Synthetic Peptide Substrates in Coagulation Testing: Some Practical Considerations for Automated Methods

Rapid Determination of Five Coagulation Parameters in One Sample with a Centrifugal Analyzer

“…From these intraindividual coefficients of variation, the biological coef ficient of variations can be calculated with the formula CV2to, = CV2assaj, + CV2bioi (ta ble IV) [22], With this biological coefficient of variation it is possible to calculate how much a future result may differ from pre vious values before there is any pathology. There is agreement with several published reports in which normal methods have been adapted to automated instruments [13,15,16,[23][24][25]. Since sample dilution, reagent consistency and precision in reagent han dling are best performed with an automated method, the performance characteristics of this instrument utilizing substrate-based methods are outstanding.…”

Evaluation of an Automated Prothrombin Assay Using a Chromogenic Substrate

“…It appeared to be less sus ceptible to the presence of heparin in plasma than the assay described by Kirchhofet al [8], but the reason for this is not clear. The preci sion of the automated assay was superior to that of the manual technique and the CV's obtained were comparable to those obtained with other automated systems [1,2,4,9], Highly significant correlations were ob tained when the assay was compared to the clotting tests which are routinely used for the control of oral anticoagulant therapy. Our estimate of the prothrombin therapeutic range is entirely consistent with that of another study (derived from Thrombotest range 5-12.5%) [10].…”

“…A number of chromogenic prothrombin assays have been described [1][2][3] and, recently, a prothrombin assay kit for use in the control of oral anticoagulant ther apy has become available [4]. Using such an assay kit, we have evaluated its perfor mance in an automated system which requires no prior dilution of the plasma samples.…”

Chromogenic microtitre tray assay for plasminogen

“…Although plasminogen assays are desirable in some clinical conditions, for example thrombolytic therapy and primary fibrinolytic disorders (Svendsen et al 1983) at the present time there are no acute clinical situations in which assessment of plasminogen level is known to be critical for patient survival.…”