Newfound Hantavirus in Chinese Mole Shrew, Vietnam

Song, Jin-Won; Kang, Hae Ji; Song, Ki-Joon; Truong, Thang T.; Bennett, Shannon N.; Arai, Satoru; Truong, Ninh Uyen; Yanagihara, Richard

doi:10.3201/eid1311.070492

Cited by 87 publications

(74 citation statements)

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“…Amplicons were separated by electrophoresis on 1.5% agarose gels and purified using the QIAQuick Gel Extraction Kit (Qiagen, Hilden, Germany), and DNA was sequenced directly using an ABI Prism 377XL Genetic Analyzer (Applied Biosystems, Foster City, CA). 8 As determined by RT-PCR and sequencing, TPMV RNA was detected in lung tissues of 12 Asian house shrews ( Tables 1 and 3 ). Of these samples, TPMV RNA was detected in kidney tissues of only four shrews, suggesting lower viral burden; 11 of 12 TPMV-infected shrews were captured in the Kathmandu district.…”

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confidence: 99%

“…2,3 The assumption that Thottapalayam virus (TPMV) represented a spillover event from a rodent reservoir has been thoroughly discredited by full-genome analysis, showing strong support for an ancient non-rodent host origin and an early evolutionary divergence from rodent-associated hantaviruses. 3,4 Moreover, the realization that shrews of multiple species (Order Soricomorpha, Family Soricidae ), including the Therese's shrew ( Crocidura theresae ), 5 Ussuri white-toothed shrew ( Crocidura lasiura ), 6 northern short-tailed shrew ( Blarina brevicauda ), 7 Chinese mole shrew ( Anourosorex squamipes ), 8 Eurasian common shrew ( Sorex araneus ), 9 masked shrew ( Sorex cinereus ), 10 dusky shrew ( Sorex monticolus ), 10 and flat-skulled shrew ( Sorex roboratus ), 11 harbor hantaviruses that are far more genetically diverse than those viruses carried by rodents suggests that shrews, being evolutionarily more ancient than rodents, may have served as the primordial hosts of ancestral hantaviruses.…”

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confidence: 99%

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Genetic Diversity of Thottapalayam Virus, a Hantavirus Harbored by the Asian House Shrew (Suncus murinus) in Nepal

Kang¹,

Kosoy²,

Shrestha³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. Despite the recent discovery of genetically divergent hantaviruses in shrews of multiple species in widely separated geographic regions, data are unavailable about the genetic diversity and phylogeography of Thottapalayam virus (TPMV), a hantavirus originally isolated from an Asian house shrew ( Suncus murinus ) captured in southern India more than four decades ago. To bridge this knowledge gap, the S, M, and L segments of hantavirus RNA were amplified by reverse transcription polymerase chain reaction from archival lung tissues of Asian house shrews captured in Nepal from January to September 1996. Pair-wise alignment and comparison revealed approximately 80% nucleotide and > 94% amino acid sequence similarity to prototype TPMV. Phylogenetic analyses, generated by maximum likelihood and Bayesian methods, showed geographic-specific clustering of TPMV, similar to that observed for rodent-and soricid-borne hantaviruses. These findings confirm that the Asian house shrew is the natural reservoir of TPMV and suggest a longstanding virus-host relationship.

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confidence: 99%

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confidence: 99%

Genetic Diversity of Thottapalayam Virus, a Hantavirus Harbored by the Asian House Shrew (Suncus murinus) in Nepal

Kang¹,

Kosoy²,

Shrestha³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…Armed with oligonucleotide primers designed on the basis of conserved regions of the TPMV genome and guided by long-ignored reports of serologic and antigenic evidence of hantavirus infection in shrews (20,33,52), we have previously detected genetically distinct hantaviruses in the Eurasian common shrew (Sorex araneus) from Switzerland (45); the Chinese mole shrew (Anourosorex squamipes) from Vietnam (46); and the northern short-tailed shrew (Blarina brevicauda), masked shrew (Sorex cinereus), and dusky shrew (Sorex monticolus) from the United States (1, 2) by reverse transcription-PCR (RT-PCR). Novel hantavirus genomes in Therese's shrew (Crocidura theresae) from Guinea (29); the vagrant shrew (Sorex vagrans), Trowbridge's shrew (Sorex trowbridgii), and the American water shrew (Sorex palustris) from the United States (H. J. Kang and R. Yanagihara, unpublished data); and the flat-skulled shrew (Sorex roboratus) and Laxmann's shrew (Sorex caecutiens) from Russia (Kang and Yanagihara, unpublished) have also been detected.…”

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confidence: 99%

Characterization of Imjin Virus, a Newly Isolated Hantavirus from the Ussuri White-Toothed Shrew ( Crocidura lasiura )

Song

Kang

et al. 2009

J Virol

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Until recently, the single known exception to the rodent-hantavirus association was Thottapalayam virus (TPMV), a long-unclassified virus isolated from the Asian house shrew (Suncus murinus)Hantaviruses (family Bunyaviridae, genus Hantavirus) are medically important rodent-borne pathogens, causing hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). The belief in long-standing coevolutionary relationships between hantaviruses and their reservoir rodent host species is supported by virus and rodent gene phylogenies. That is, phylogenetic analyses, based on full-length viral genomic sequences and rodent mitochondrial DNA (mtDNA) or nuclear gene sequences, indicate that antigenically distinct hantaviruses segregate into clades, which parallel the evolution of rodents in the subfamilies Murinae, Arvicolinae, Neotominae, and Sigmodontinae (23,25,26,28,39,54). Previously, this phylogenetic insight has been successfully employed to direct the discovery of new hantaviruses, such as those found in the Korean field mouse (Apodemus peninsulae) (5) and the royal vole (Myodes regulus) (47).Renewed interest in the role of nonrodent reservoirs in the evolution of hantaviruses has been spurred by recent analysis of the entire genome of Thottapalayam virus (TPMV), a hantavirus isolated from the Asian house shrew (Suncus murinus) (10, 61), which revealed a separate phylogenetic clade, suggesting early evolutionary divergence from rodent-borne hantaviruses (44, 56). Armed with oligonucleotide primers designed on the basis of conserved regions of the TPMV genome and guided by long-ignored reports of serologic and antigenic evidence of hantavirus infection in shrews (20, 33, 52), we have previously detected genetically distinct hantaviruses in the Eurasian common shrew (Sorex araneus) from Switzerland (45); the Chinese mole shrew (Anourosorex squamipes) from Vietnam (46); and the northern short-tailed shrew (Blarina brevicauda), masked shrew (Sorex cinereus), and dusky shrew (Sorex monticolus) from the United States (1, 2) by reverse transcription-PCR (RT-PCR). Novel hantavirus genomes in Therese's shrew (Crocidura theresae) from Guinea (29); the vagrant shrew (Sorex vagrans), Trowbridge's shrew (Sorex trowbridgii), and the American water shrew (Sorex palustris) from the United States (H. J. Kang and R. Yanagihara, unpublished data); and the flat-skulled shrew (Sorex roboratus) and Laxmann's shrew (Sorex caecutiens) from Russia (Kang and Yanagihara, unpublished) have also been detected.Here, we report the antigenic, genetic, and phylogenetic characterization of a newly identified hantavirus, designated Imjin virus (MJNV), isolated from Ussuri white-toothed shrews of the species Crocidura lasiura (order Soricomorpha, family Soricidae, subfamily Crocidurinae) captured near the demilitarized zone (DMZ) in the Republic of Korea. The discovery of MJNV and other soricid-borne hantaviruses from widely separated geographic regions, spanning four continents,

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“…Moreover, the recent discovery of genetically diverse hantaviruses in shrews of multiple species (order Soricomorpha, family Soricidae), including Tanganya virus in the Therese's shrew (Crocidura theresae) (27), Imjin virus in the Ussuri whitetoothed shrew (Crocidura lasiura) (49), Camp Ripley virus in the northern short-tailed shrew (Blarina brevicauda) (4), Cao Bang virus in the Chinese mole shrew (Anourosorex squamipes) (48), Seewis virus in the Eurasian common shrew (Sorex araneus) (47), Ash River virus in the masked shrew (Sorex cinereus) (2), Jemez Springs virus in the dusky shrew (Sorex monticolus) (2), and Kenkeme virus in the flat-skulled shrew (Sorex roboratus) (25), as well as in moles (family Talpidae), including Asama virus (ASAV) in the Japanese shrew mole (Urotrichus talpoides) (3), Oxbow virus (OXBV) in the American shrew mole (Neurotrichus gibbsii) (23), and Nova virus (NVAV) in the European common mole (Talpa europaea) (24), suggests that the evolutionary history of hantaviruses is more complex than previously conjectured.…”

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Shared Ancestry between a Newfound Mole-Borne Hantavirus and Hantaviruses Harbored by Cricetid Rodents

Kang

Bennett

Hope

et al. 2011

J Virol

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Discovery of genetically distinct hantaviruses in multiple species of shrews (order Soricomorpha, family Soricidae) and moles (family Talpidae) contests the conventional view that rodents (order Rodentia, families Muridae and Cricetidae) are the principal reservoir hosts and suggests that the evolutionary history of hantaviruses is far more complex than previously hypothesized. We now report on Rockport virus (RKPV), a hantavirus identified in archival tissues of the eastern mole (Scalopus aquaticus) collected in Rockport, TX, in 1986. Pairwise comparison of the full-length S, M, and L genomic segments indicated moderately low sequence similarity between RKPV and other soricomorph-borne hantaviruses. Phylogenetic analyses, using maximumlikelihood and Bayesian methods, showed that RKPV shared a most recent common ancestor with cricetidrodent-borne hantaviruses. Distributed widely across the eastern United States, the fossorial eastern mole is sympatric and syntopic with cricetid rodents known to harbor hantaviruses, raising the possibility of hostswitching events in the distant past. Our findings warrant more-detailed investigations on the dynamics of spillover and cross-species transmission of present-day hantaviruses within communities of rodents and moles.

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Newfound Hantavirus in Chinese Mole Shrew, Vietnam

Cited by 87 publications

References 4 publications

Genetic Diversity of Thottapalayam Virus, a Hantavirus Harbored by the Asian House Shrew (Suncus murinus) in Nepal

Genetic Diversity of Thottapalayam Virus, a Hantavirus Harbored by the Asian House Shrew (Suncus murinus) in Nepal

Characterization of Imjin Virus, a Newly Isolated Hantavirus from the Ussuri White-Toothed Shrew ( Crocidura lasiura )

Shared Ancestry between a Newfound Mole-Borne Hantavirus and Hantaviruses Harbored by Cricetid Rodents

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