Newly discovered genes for resistance to powdery mildew in the subtelomeric region of the short arm of barley chromosome 7H

Soldánová, Martina; Ištvánek, Jan; Řepková, Jana; Dreisetl, Antonín

doi:10.17221/33/2013-cjgpb

Cited by 9 publications

(3 citation statements)

References 18 publications

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“…The resistance gene cluster Mla located on chromosome 1H is another major resistance locus against PM [ 6 ]. This gene has been detected in many other studies [ 86 , 87 , 88 , 89 , 90 , 91 , 92 ] and was also detected in the present study. The region Qrbg_1H_1 was significant in all populations and is located on chromosome 1HS between 6.9 and 10.2 Mbp.…”

Section: Discussionsupporting

confidence: 89%

You Had Me at “MAGIC”!: Four Barley MAGIC Populations Reveal Novel Resistance QTL for Powdery Mildew

Novakazi

Krusell

Jensen³

et al. 2020

Genes

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Blumeria graminis f. sp. hordei (Bgh), the causal agent of barley powdery mildew (PM), is one of the most important barley leaf diseases and is prevalent in most barley growing regions. Infection decreases grain quality and yields on average by 30%. Multi-parent advanced generation inter-cross (MAGIC) populations combine the advantages of bi-parental and association panels and offer the opportunity to incorporate exotic alleles into adapted material. Here, four barley MAGIC populations consisting of six to eight founders were tested for PM resistance in field trials in Denmark. Principle component and STRUCTURE analysis showed the populations were unstructured and genome-wide linkage disequilibrium (LD) decay varied between 14 and 38 Mbp. Genome-wide association studies (GWAS) identified 11 regions associated with PM resistance located on chromosomes 1H, 2H, 3H, 4H, 5H and 7H, of which three regions are putatively novel resistance quantitative trait locus/loci (QTL). For all regions high-confidence candidate genes were identified that are predicted to be involved in pathogen defense. Haplotype analysis of the significant SNPs revealed new allele combinations not present in the founders and associated with high resistance levels.

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Section: Discussionsupporting

confidence: 89%

You Had Me at “MAGIC”!: Four Barley MAGIC Populations Reveal Novel Resistance QTL for Powdery Mildew

Novakazi

Krusell

Jensen³

et al. 2020

Genes

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“…DNA markers have a broad spectrum of use in both research and practice. They are used for QTL mapping ( Řepková et al, 2006a ; Soldánová et al, 2013 ; Zhao et al, 2013 ), the deduction of evolution relationships ( Isobe et al, 2012 ), variability assessment and genotypization of primary breeding material ( Younas et al, 2012 ; Cidade et al, 2013 ), marker-assisted selection in breeding generations and even gene pyramiding ( Qi et al, 2015 ). Based on NGS technology, the number of newly discovered DNA markers substantially increased ( Zalapa et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Red Clover (Trifolium pratense) and Zigzag Clover (T. medium) – A Picture of Genomic Similarities and Differences

Dluhošová

Ištvánek

Nedĕlník³

et al. 2018

Front. Plant Sci.

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The genus clover (Trifolium sp.) is one of the most economically important genera in the Fabaceae family. More than 10 species are grown as manure plants or forage legumes. Red clover’s (T. pratense) genome size is one of the smallest in the Trifolium genus, while many clovers with potential breeding value have much larger genomes. Zigzag clover (T. medium) is closely related to the sequenced red clover; however, its genome is approximately 7.5x larger. Currently, almost nothing is known about the architecture of this large genome and differences between these two clover species. We sequenced the T. medium genome (2n = 8x = 64) with ∼23× coverage and managed to partially assemble 492.7 Mbp of its genomic sequence. A thorough comparison between red clover and zigzag clover sequencing reads resulted in the successful validation of 7 T. pratense- and 45 T. medium-specific repetitive elements. The newly discovered repeats led to the set-up of the first partial T. medium karyotype. Newly discovered red clover and zigzag clover tandem repeats were summarized. The structure of centromere-specific satellite repeat resembling that of T. repens was inferred in T. pratense. Two repeats, TrM300 and TrM378, showed a specific localization into centromeres of a half of all zigzag clover chromosomes; TrM300 on eight chromosomes and TrM378 on 24 chromosomes. A comparison with the red clover draft sequence was also used to mine more than 105,000 simple sequence repeats (SSRs) and 1,170,000 single nucleotide variants (SNVs). The presented data obtained from the sequencing of zigzag clover represent the first glimpse on the genomic sequence of this species. Centromeric repeats indicated its allopolyploid origin and naturally occurring homogenization of the centromeric repeat motif was somehow prevented. Using various repeats, highly uniform 64 chromosomes were separated into eight types of chromosomes. Zigzag clover genome underwent substantial chromosome rearrangements and cannot be counted as a true octoploid. The resulting data, especially the large number of predicted SSRs and SNVs, may have great potential for further research of the legume family and for rapid advancements in clover breeding.

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“…Moreover, in seven accessions [ 12 , 16 , 92 , 93 , 94 ], dominant genes were located on chromosome 1HS (probably in Mla locus), and three accessions [ 12 , 93 , 94 ] contained dominant genes on chromosome 2HS. In three other accessions [ 12 , 92 , 95 ], genes on 7HS were detected, and in one accession [ 12 ], a gene on chromosome 7HL was localized.…”

Section: Specific Resistancementioning

confidence: 99%

Specific Resistance of Barley to Powdery Mildew, Its Use and Beyond: A Concise Critical Review

Dreiseitl

2020

Genes

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Powdery mildew caused by the airborne ascomycete fungus Blumeria graminis f. sp. hordei (Bgh) is one of most common diseases of barley (Hordeum vulgare). This, as with many other plant pathogens, can be efficiently controlled by inexpensive and environmentally-friendly genetic resistance. General requirements for resistance to the pathogens are effectiveness and durability. Resistance of barley to Bgh has been studied intensively, and this review describes recent research and summarizes the specific resistance genes found in barley varieties since the last conspectus. Bgh is extraordinarily adaptable, and some commonly recommended strategies for using genetic resistance, including pyramiding of specific genes, may not be effective because they can only contribute to a limited extent to obtain sufficient resistance durability of widely-grown cultivars. In spring barley, breeding the nonspecific mlo gene is a valuable source of durable resistance. Pyramiding of nonspecific quantitative resistance genes or using introgressions derived from bulbous barley (Hordeum bulbosum) are promising ways for breeding future winter barley cultivars. The utilization of a wide spectrum of nonhost resistances can also be adopted once practical methods have been developed.

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Newly discovered genes for resistance to powdery mildew in the subtelomeric region of the short arm of barley chromosome 7H

Cited by 9 publications

References 18 publications

You Had Me at “MAGIC”!: Four Barley MAGIC Populations Reveal Novel Resistance QTL for Powdery Mildew

You Had Me at “MAGIC”!: Four Barley MAGIC Populations Reveal Novel Resistance QTL for Powdery Mildew

Red Clover (Trifolium pratense) and Zigzag Clover (T. medium) – A Picture of Genomic Similarities and Differences

Specific Resistance of Barley to Powdery Mildew, Its Use and Beyond: A Concise Critical Review

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