Newly Isolated Lactobacilli strains from Algerian Human Vaginal Microbiota: Lactobacillus fermentum Strains Relevant Probiotic’s Candidates

Abstract

Lactobacilli strains are considered as a preventive means for treatment of vaginal infections or post-antibiotic treatment to repopulate the vaginal mucosa. This study aimed at establishing the vaginal lactobacillus profile of Algerian women with different vaginal diseases. Afterwards, lactobacilli isolated from swabs were in vitro characterized for their probiotic hallmarks. This prospective study allowed isolation of 44 Lactobacillus strains and 160 potentially pathogens, among which are Escherichia coli (50 isolates), Staphylococcus sp. (38 isolates), Enterococcus sp. (16 isolates), and Candida sp. (56 isolates). All Lactobacilli strains were characterized for their antagonism, adhesion to polystyrene, and resistance to acidity and bile. Consequently, six Lactobacillus strains (Lb. fermentum 5LB4, 5LB10, 5LB12, Lb. plantarum 5LB2, 5LB11, and Lactobacillus sp. 4LB9) were moderately or weakly adherent, and 35 potentially pathogens exhibited weak to strong adhesion to polystyrene. Antagonism was recorded for 36 Lactobacillus strains towards E. coli 6E2, S. aureus 7S3, Enterococcus sp. 5EN8, and Candida albicans C1 used as indicator organisms. Finally, Lb. fermentum 9LB6, 4LB16, and 10LB1 and Lb. plantarum 9LB4 were remarkable for their inhibitory activity, absence of hemolytic potential, and for their resistance to acidity (pH 1.5) and bile (0.5%) harsh conditions.

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

“…Bacterial autoaggregation, defined as the ability of cells to form precipitates, is considered an important probiotic property, as it is directly related to adhesion to host cell surfaces, one of the mechanisms by which probiotics compete with pathogenic bacteria [ 24 ]. These values were within the expected range, since there is great variation in autoaggregation among strains of both human vaginal microbiota and nonhuman origin [ 25 , 26 ]. Gómez et al [ 27 ] found autoaggregation values ranging from 20 to 70%, after only 24 h of incubation, among 8 strains of LAB isolated from different food sources, and the highest percentage, 67%, was a Weissella viridescens strain isolated from mature cheese.…”

The Role of the Cervicovaginal Microbiome on the Genesis and as a Biomarker of Premalignant Cervical Intraepithelial Neoplasia and Invasive Cervical Cancer

“…L. plantarum and L. fermentum strains isolated from healthy Cuban women exhibited antagonic activity against G. vaginalis, C. albicans, as well as remarkable adhesive capacities and lactic acid production [134]. Similarly, L. fermentum 9LB6, 4LB16, and 10LB1 and L. plantarum 9LB4 strains were isolated from Algerian women and suggested as probiotic candidates based on their relevant inhibitory activity of pathogens, lack of hemolytic capability and other beneficial properties according to in vitro studies [135]. However, the reports mentioned above lack clinical evidence.…”

Anticandidal Activity of Omiganan and Its Retro Analog Alone and in Combination with Fluconazole

“…Among the compounds which are worth mentioning are those synthesized by bacteria, which are characterized by probiotic as well as antimicrobial activity, also against Candida. Among the microorganisms with probiotic anti-Candida potential, there are mainly bacteria of the genus Lactobacillus, including L. acidophilus, L. rhamnosum, L. crispatus, L. fermentum, and L. brevis [11][12][13][14][15][16]. Besides their probiotic potential, these strains are characterized by production of variety antimicrobial compounds like organic acids, ethanol, enzymes, and hydrogen peroxide andantimicrobial peptides (AMPs) [17,18].…”