News &amp; Notes: Virulence of Vibrio alginolyticus Isolated from Diseased Tiger Prawn, Penaeus monodon

Lee, Kuo‐Kau; Yu, Shu-Ru; Chen, Ferng-Ruey; Yang, Tun-I; Liu, Ping-Chung

doi:10.1007/s002849900041

Cited by 130 publications

(74 citation statements)

References 13 publications

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“…Other Vibrio spp. (V. alginolyticus, V. tubiashii, V. campbellii) cultured from turtles in this study are known pathogens of molluscs (Hada et al 1984, Zheng et al 1991, crustaceans (Lee et al 1996, Sahul Hameed et al 1996, and fish (Austin et al 1993). Vibrio spp.…”

Section: Discussionmentioning

confidence: 99%

Bacteraemia in free-ranging Hawaiian green turtles Chelonia mydas with fibropapillomatosis

Work

Balazs²,

Wolcott³

et al. 2003

Dis. Aquat. Org.

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Past studies of free-ranging green turtles Chelonia mydas with fibropapillomatosis (FP) in Hawaii have shown that animals become immunosuppressed with increasing severity of this disease. Additionally, preliminary clinical examination of moribund turtles with FP revealed that some animals were also bacteraemic. We tested the hypothesis that bacteraemia in sea turtles is associated with the severity of FP. We captured free-ranging green turtles from areas in Hawaii where FP is absent, and areas where FP has been endemic since the late 1950s. Each turtle was given an FP severity score ranging from 0 (no tumours) to 3 (severely affected). A fifth category included turtles that were stranded ashore and moribund with FP. We found that the percentage of turtles with bacteraemia increased with the severity of FP, and that the majority of bacteria cultured were Vibrio spp. Turtles with severe FP were more susceptible to bactaeremia, probably in part due to immunosuppression. The pattern of bacteraemia in relation to severity of disease strengthens the hypothesis that immunosuppression is a sequel to FP.

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Section: Discussionmentioning

confidence: 99%

Bacteraemia in free-ranging Hawaiian green turtles Chelonia mydas with fibropapillomatosis

Work

Balazs²,

Wolcott³

et al. 2003

Dis. Aquat. Org.

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“…V. anguillarum, V. salmonicida, and V. vulnificus are among the main bacterial pathogens of several fish species (14), and V. harveyi is a major pathogen of shrimp, e.g., Litopenaeus vannamei and Penaeus monodon (15,225,227). Mortality caused by vibrios in reared fish and shellfish is very common during early larval stages and can occur suddenly, leading sometimes to death of the entire population (42,96,157,184,185,187,217,230,283,287,295). Pathogenicity of V. harveyi is associated with the presence of a bacteriophage (15,291).…”

Section: Role In Aquaculturementioning

confidence: 99%

Biodiversity of Vibrios

Thompson

Iida

Swings

2004

Microbiol Mol Biol Rev

1,070

888

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Vibrios are ubiquitous and abundant in the aquatic environment. A high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals, fish, shrimp, sponges, squid, and zooplankton. Vibrios harbour a wealth of diverse genomes as revealed by different genomic techniques including amplified fragment length polymorphism, multilocus sequence typing, repetetive extragenic palindrome PCR, ribotyping, and whole-genome sequencing. The 74 species of this group are distributed among four different families, i.e., Enterovibrionaceae, Photobacteriaceae, Salinivibrionaceae, and Vibrionaceae. Two new genera, i.e., Enterovibrio norvegicus and Grimontia hollisae, and 20 novel species, i.e., Enterovibrio coralii, Photobacterium eurosenbergii, V. brasiliensis, V. chagasii, V. coralliillyticus, V. crassostreae, V. fortis, V. gallicus, V. hepatarius, V. hispanicus, V. kanaloaei, V. neonatus, V. neptunius, V. pomeroyi, V. pacinii, V. rotiferianus, V. superstes, V. tasmaniensis, V. ezurae, and V. xuii, have been described in the last few years. Comparative genome analyses have already revealed a variety of genomic events, including mutations, chromosomal rearrangements, loss of genes by decay or deletion, and gene acquisitions through duplication or horizontal transfer (e.g., in the acquisition of bacteriophages, pathogenicity islands, and super-integrons), that are probably important driving forces in the evolution and speciation of vibrios. Whole-genome sequencing and comparative genomics through the application of, e.g., microarrays will facilitate the investigation of the gene repertoire at the species level. Based on such new genomic information, the taxonomy and the species concept for vibrios will be reviewed in the next years

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“…Several clinical infections caused by V. alginolyticus were reported (Levine and Griffin 1993;Reina Prieto and Heravs Palazon 1993;GahrnHansen and Hornstrup 1994;Gomez et al 2003), as well as tremendous damage caused in fish, shellfish and crustaceans (Anderson et al 1988;Lightner 1988;Lee et al 1996;Vandenberghe et al 1999;Hörmansdorfer et al 2000;Huang and He 2002;George et al 2005;Gó mezLeó n et al 2005;Hu et al 2005;Jayaprakash et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Development of a SYBR Green I real-time PCR for quantitative detection of Vibrio alginolyticus in seawater and seafood

Zhou

Hou

et al. 2007

Journal of Applied Microbiology

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Aim: Vibrio alginolyticus is an economically important micro‐organism. The main aim of the present study was to develop a real‐time polymerase chain reaction (PCR) assay for rapid, sensitive and effective quantification of V. alginolyticus in seawater and seafood. Methods and Results: Purified DNA of V. alginolyticus, artificially inoculated seawater and seafood tissue homogenates were subjected to the gyrB‐targeted real‐time PCR assay. Natural seawater and seafood samples were analysed by this real‐time PCR protocol. Specificity tests showed that positive result was obtained only with V. alginolyticus strains. The detection sensitivity was determined to be 0·4 pg of genomic DNA equivalent to 72 cells per PCR in pure culture and 100 cells in 1 ml of seawater or seafood tissue homogenates. Single cell detection is achieved after 3 h of sample enrichment. Conclusions: A sensitive and specific SYBR Green I‐based real‐time PCR assay targeting gyrB gene was successfully developed to quantify V. alginolyticus within 6 h in seawater and seafood samples. Significance and Impact of the Study: No report on the molecular‐based method was available for quantitative detection of V. alginolyticus. This work will provide a novel method for evaluation of the risk of V. alginolyticus to marine environmental health and seafood safety.

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News & Notes: Virulence of Vibrio alginolyticus Isolated from Diseased Tiger Prawn, Penaeus monodon

Cited by 130 publications

References 13 publications

Bacteraemia in free-ranging Hawaiian green turtles Chelonia mydas with fibropapillomatosis

Bacteraemia in free-ranging Hawaiian green turtles Chelonia mydas with fibropapillomatosis

Biodiversity of Vibrios

Development of a SYBR Green I real-time PCR for quantitative detection of Vibrio alginolyticus in seawater and seafood

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