Next-generation diagnostic test for dengue virus detection using an ultrafast plasmonic colorimetric RT-PCR strategy

A novel colorimetric biosensor for rapid detection of dengue virus upon acid-induced aggregation of colloidal gold

“…As shown in the table, this sensing platform is more sensitive for the analysis of nucleic acid of RNA viruses than other sensors, such as methods for detection of nucleic acid of West Nile Virus employing a biosensor using a surface-enhanced Raman scattering sensor with gold-coated paramagnetic nanoparticles (6.11 × 10 9 copies per reaction), 51 and phytochip using a new SPR platform for RNA virus detection (3.23 × 10 8 copies per reaction), 53 proving its great potential for early detection of diseases. Although the sensitivity of our method was lower than that of the available amplification-based methods for detecting nucleic acids of RNA viruses, such as RT-LAMP-based colorimetric biosensors using chitosan-capped AuNPs in a single-tube assay (10 copies per reaction), 55 RT-PCR-based colorimetric biosensors (1.6 copies per reaction), 54 and the colorimetric sandwich-type bioassay using an hACE2-based affinity peptide pair (1.25 × 10 3 –1.25 × 10 5 copies per reaction), 52 these procedures involve sample pre-treatment, amplification, high cost, complexity, and specialized equipment for measurement, and are incapable of on-site testing. 56–58 Compared with these available methods, our suggested biosensors are convenient in terms of versatility, simplicity, affordable experimental cost, quick analysis, and the capacity to do point-of-care testing of target genomic RNA.…”

The Development of New Primer Sets for the Amplification and Sequencing of the Envelope Gene of All Dengue Virus Serotypes

“…These include the standard RT-PCR [ 35 ] and multiplex RT-PCR, which can detect and differentiate serotypes [ 36 ]. Other innovative methods include the use of a multiplexed PCR chip platform with an ion-selective membrane sensor [ 37 ] or a plasmonic colorimetric RT-PCR strategy [ 38 ]. The latest diagnostic methods have proven extremely sensitive and can even detect around 100 copies/μL of viral nucleic acid.…”