2023
DOI: 10.1242/bio.060015
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Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms

Frederic Strobl,
Julia Ratke,
Franziska Krämer
et al.

Abstract: For diploid model organisms, the actual transgenesis processes require subsequent periods of transgene management, which are challenging in emerging model organisms due to the lack of suitable methodology. We used the red flour beetle Tribolium castaneum, a stored-grain pest, to perform a comprehensive functional evaluation of our AClashOfStrings (ACOS) and the combined AGameOfClones/AClashOfStrings (AGOC/ACOS) vector concepts, which use four clearly distinguishable markers to provide full visual control over … Show more

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