Next generation of neurological therapeutics: Native and bioengineered extracellular vesicles derived from stem cells

Jin, Shilin; Lv, Zhongyue; Kang, Lin; Wang, Jiayi; Tan, Chibing; Shen, Liming; Wang, Liang; Liu, Jing

doi:10.1016/j.ajps.2022.10.002

Cited by 8 publications

(4 citation statements)

References 168 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 167 Several strategies are necessary to enhance the potential therapeutic effects of MSC‐EVs, such as,: (I) developing platforms for large‐scale, cost‐effective production of MSC‐EVs to meet clinical needs; (II) improving detection methods for MSC‐EVs to determine the optimal dosage, frequency, and tissue distribution; and (III) enhancing modified strategies of MSC‐EVs to improve their biological functions and target site accumulation. 168 Exosomes as DDSs offer a range of advantages: biocompatibility, biodegradability, intercellular communication capabilities, targeted delivery, immunomodulatory properties, low immunogenicity, low toxicity, and potential for personalized medicine. Among these, the potential for personalized medicine is an exciting aspect of exosomes.…”

Section: Discussionmentioning

confidence: 99%

“…Establishing a mature, stable, convenient, and fast method for the separation of exosomes is a premise for their use in and (III) enhancing modified strategies of MSC-EVs to improve their biological functions and target site accumulation. 168 Exosomes as DDSs offer a range of advantages: biocompatibility, biodegradability, intercellular communication capabilities, targeted delivery, immunomodulatory properties, low immunogenicity, low toxicity, and potential for personalized medicine. Among these, the potential for personalized medicine is an exciting aspect of exosomes.…”

Section: Cell-free Therapy Based On Stem Cell-derived Exosomesmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and usage of exosomes in central nervous system diseases

Wang,

Sun,

Duan

et al. 2024

CNS Neurosci Ther

View full text Add to dashboard Cite

BackgroundExosomes are vesicles secreted by all types of mammalian cells. They are characterized by a double‐layered lipid membrane structure. They serve as carriers for a plethora of signal molecules, including DNA, RNA, proteins, and lipids. Their unique capability of effortlessly crossing the blood–brain barrier underscores their critical role in the progression of various neurological disorders. This includes, but is not limited to, diseases such as Alzheimer's, Parkinson's, and ischemic stroke. Establishing stable and mature methods for isolating exosomes is a prerequisite for the study of exosomes and their biomedical significance. The extraction technologies of exosomes include differential centrifugation, density gradient centrifugation, size exclusion chromatography, ultrafiltration, polymer coprecipitation, immunoaffinity capture, microfluidic, and so forth. Each extraction technology has its own advantages and disadvantages, and the extraction standards of exosomes have not been unified internationally.AimsThis review aimed to showcase the recent advancements in exosome isolation techniques and thoroughly compare the advantages and disadvantages of different methods. Furthermore, the significant research progress made in using exosomes for diagnosing and treating central nervous system (CNS) diseases has been emphasized.ConclusionThe varying isolation methods result in differences in the concentration, purity, and size of exosomes. The efficient separation of exosomes facilitates their widespread application, particularly in the diagnosis and treatment of CNS diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Cell-free Therapy Based On Stem Cell-derived Exosomesmentioning

confidence: 99%

Isolation and usage of exosomes in central nervous system diseases

Wang,

Sun,

Duan

et al. 2024

CNS Neurosci Ther

View full text Add to dashboard Cite

show abstract

“…[21][22][23] Endowing them with physicochemical properties by nanomodification on the membrane to generate engineered living cell-based biotherapeutic nanorobots may hold great promise on inhibiting tumor progress in a safer manner. [24][25][26] Multiple attempts have been made to functionalize these biological vectors through biomimetic nanotechnology, [27,28] which not only overcomes the disadvantage of low biocompatibility of nanoparticles, but also fully improves the functionalization of living cell biological systems, thus exhibiting a synergistically enhanced precision anti-tumor therapeutic effect. [29] There are currently several proven methods for loading nanoparticles by living cell carriers.…”

Section: Introductionmentioning

confidence: 99%

Living Cell‐Derived Intelligent Nanobots for Precision Oncotherapy

Zhou,

Li,

Liu

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

The progress of precision oncology medicine is always limited by the tumor off‐targeting, the drug side effects, and the treatment inefficiency due to the complex and ever‐changing tumor microenvironment. Living cells, such as blood cells and immune cells, exhibit natural tumor tropism, controllable physicochemical modification, and excellent biocompatibility, which provide an advantageous pathway for innovative and efficient tumor suppression. Armed with nanoengineering techniques, artificial living cells harness their inherent biological properties to precisely identify and eradicate tumors, demonstrating broad biological application prospects and great transformational potential in personalized cancer therapy. Here, the recent advances of living cell‐based bionanobots including platelets, red blood cells, neutrophil, macrophage, and CAR‐T cells for cancer precision therapy and immune regulation are summarized, and the efficient anti‐tumor strategies for engineering living cell nanorobots to overcome complex biological barriers and immune suppression are also outlined (e.g., immunotherapy, sonodynamic therapy, chemo/radiotherapy, and phototherapy). In addition, the study discusses the advantages, limitations, and current challenges of artificial living cell‐based drug delivery systems, and provide perspectives on the future development of living cell‐mediated precision tumor nanomedicine.

show abstract

“…Several cell surface CAMs, such as CD44 and PECAM-1, have been identified with antiphagocytic functions against macrophages, have also been considered candidates for modifying EVs and thus increase their circulation half-life [ 153 ]. In addition, changing the biodistribution of EVs is an important strategy to ensure that they act on target tissues [ 154 ]. As previously mentioned in this paper, the expression of specific CAMs carried by EVs confer EVs with the ability to target specific tissues.…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicle-cell adhesion molecules in tumours: biofunctions and clinical applications

Lin,

Fang,

Wei

et al. 2023

Cell Commun Signal

View full text Add to dashboard Cite

Cell adhesion molecule (CAM) is an umbrella term for several families of molecules, including the cadherin family, integrin family, selectin family, immunoglobulin superfamily, and some currently unclassified adhesion molecules. Extracellular vesicles (EVs) are important information mediators in cell-to-cell communication. Recent evidence has confirmed that CAMs transported by EVs interact with recipient cells to influence EV distribution in vivo and regulate multiple cellular processes. This review focuses on the loading of CAMs onto EVs, the roles of CAMs in regulating EV distribution, and the known and possible mechanisms of these actions. Moreover, herein, we summarize the impacts of CAMs transported by EVs to the tumour microenvironment (TME) on the malignant behaviour of tumour cells (proliferation, metastasis, immune escape, and so on). In addition, from the standpoint of clinical applications, the significance and challenges of using of EV-CAMs in the diagnosis and therapy of tumours are discussed. Finally, considering recent advances in the understanding of EV-CAMs, we outline significant challenges in this field that require urgent attention to advance research and promote the clinical applications of EV-CAMs.

show abstract

Next generation of neurological therapeutics: Native and bioengineered extracellular vesicles derived from stem cells

Cited by 8 publications

References 168 publications

Isolation and usage of exosomes in central nervous system diseases

Isolation and usage of exosomes in central nervous system diseases

Living Cell‐Derived Intelligent Nanobots for Precision Oncotherapy

Extracellular vesicle-cell adhesion molecules in tumours: biofunctions and clinical applications

Contact Info

Product

Resources

About