Next Generation Sequence-based Technologies for Analyzing DNA Strand Breaks

Abstract

Genomic DNA is constantly exposed to various types of exogenous and endogenous stimulants that induce DNA lesions, including single-strand breaks (SSBs) and double-strand breaks (DSBs). Unrepaired DNA damages eventually cause adverse effects on a wide range of cellular and physiological processes; therefore, it is of great interest to map the damaged and repaired DNA to elucidate the damage distribution on a genome-wide scale. In the past decade, several sequence-based approaches for detection and quantification of such modified DNA have been established via technological innovation in sequencing analysis, which have expanded our understanding of DNA damage and repair. This review provides an overview of next-generation sequence-based methods for damaged DNA analysis with a focus on DNA strand breaks, SSBs, and DSBs.

Optical design of the N.A.1.1 infinite conjugate microscopic objective

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Horizontal gene transfer in eukaryotes: A biological phenomenon with unknown molecular mechanisms but accumulating DNA evidence

“…With the remarkable improvement in genomic analysis technologies, molecular biology techniques directly targeting DNA became integrated into research on HGT around the 1990s (Arauchi et al 2014). The advent of next-generation sequencers in 2005 enabled the rapid revelation of genomic sequences of eukaryotes (Fujiwara et al 2021). These next-generation sequencers can decode approximately 1 billion bases in a single run, surpassing the complexity of genomes of bacteria and facilitating the decoding of genomic sequences of eukaryotes with ease (Arauchi et al 2014).…”