Next generation sequencing: an application in forensic sciences?

Álvarez-Cubero, María Jesús; Saiz, María; Martínez-García, Belén; Sayalero, Sara M.; Entrala, Carmen; Lorente, José A.; Martínez-González, Luis Javier

doi:10.1080/03014460.2017.1375155

Cited by 56 publications

(45 citation statements)

References 56 publications

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“…Next Generation Sequencing (NGS), may provide advantages in the use of autosomal SNPs for forensic analysis 19,21 to perform parallel genotyping of large numbers of SNPs, which can successfully deal with small amounts of degraded DNA 19 , as is shown in the present study.…”

Section: Introductionmentioning

confidence: 76%

“…Research shows the increasing interest in petrous bones as a strategic region to recover DNA, even though these studies are focused solely on Sequence-Tagged Sites and Short Tandem Repeats (STRs) typing to obtain a match between DNA profiles (DNA profiling). The use of autosomal Single-Nucleotide Polymorphisms (SNPs) in forensic identification is of great relevance especially in the case of forensic DNA phenotyping 19 . A recent study by Kulstein et al .…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide DNA from Degraded Petrous Bones and the Assessment of Sex and Probable Geographic Origins of Forensic Cases

Gaudio

Fernandes

Schmidt

et al. 2019

Sci Rep

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The acquisition of biological information and assessment of the most probable geographic origin of unidentified individuals for obtaining positive identification is central in forensic sciences. Identification based on forensic DNA, however, varies greatly in relation to degradation of DNA. Our primary aim is to assess the applicability of a petrous bone sampling method in combination with Next Generation Sequencing to evaluate the quality and quantity of DNA in taphonomically degraded petrous bones from forensic and cemetery cases. A related aim is to analyse the genomic data to obtain the molecular sex of each individual, and their most probable geographic origin. Six of seven subjects were previously identified and used for comparison with the results. To analyse their probable geographic origin, samples were genotyped for the 627.719 SNP positions. Results show that the inner ear cochlear region of the petrous bone provides good percentages of endogenous DNA (14.61–66.89%), even in the case of burnt bodies. All comparisons between forensic records and genetic results agree (sex) and are compatible (geographic origin). The application of the proposed methodology may be a powerful tool for use in forensic scenarios, ranging from missing persons to unidentified migrants who perish when crossing borders.

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Section: Introductionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Genome-Wide DNA from Degraded Petrous Bones and the Assessment of Sex and Probable Geographic Origins of Forensic Cases

Gaudio

Fernandes

Schmidt

et al. 2019

Sci Rep

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“…Despite the relative youth of forensic genetics, several generations of methods have already been developed and obsoleted in this field. Recently, sequencing techniques have continued to move ahead at a staggering speed, and the massive parallel sequencing strategy, capable of simultaneously generating thousands of reads on state-of-art devices, will revolutionize genetic analysis [277,278]. Although the admissibility of MPS results in a court of law will most likely continue to be challenged-as are mostly newly introduced methodsforeseeably raising concerns about privacy [278], the complementary application of MPS genome-wide data, with other innovative and advanced analytical systems-e.g., imaging analysis-can extend the professionalism and efficiency of the multidisciplinary medicolegal field to the ultimate benefit of society [278].…”

Section: Discussionmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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“…Для этого можно использовать как традиционные реляционные Таблица 1. Геномные маркеры идентификации (CCП -секвенирование следующего поколения) [51] [37]. ДНК-анализ КТП широко применятся в лабораториях по всему миру для задач практической судебной экспертизы.…”

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“…Такая ситуация бывает в случаях разложения ДНК по причине давнего срока смер-Таблица 2. Основные лабораторные технологии анализа ДНК для целей криминалистики и судебной медицины [51] Table 2. Basic laboratory DNA analysis technologies for the purposes of forensic science and forensic medicine [51] ты i-того аллеля внутри двух популяций А и В, которые сравниваются по локусам генов [49].…”

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Сhallenging Questions of Development and Application of DNA Banks for the Purposes of Criminology and Related Disciplines

Sprindzuk

Titov

Кончиц

2019

Cifrovaja transformacija

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Review article presents essential information on DNA databases, forensic genomics for human identification and suspect characteristics. Author reports the essential information on the topic of forensic DNA databases and data processing. DNA databases are important tools for the improvement of performance of the security organizations and services with a final goal of national security enhancement.

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Next generation sequencing: an application in forensic sciences?

Cited by 56 publications

References 56 publications

Genome-Wide DNA from Degraded Petrous Bones and the Assessment of Sex and Probable Geographic Origins of Forensic Cases

Genome-Wide DNA from Degraded Petrous Bones and the Assessment of Sex and Probable Geographic Origins of Forensic Cases

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Сhallenging Questions of Development and Application of DNA Banks for the Purposes of Criminology and Related Disciplines

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