Next-generation sequencing and waste/wastewater treatment: a comprehensive overview

Sanz, J. L.; Köchling, Thorsten

doi:10.1007/s11157-019-09513-0

Cited by 34 publications

(14 citation statements)

References 191 publications

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“…Only a small fraction of microbial diversity has been studied so far due to the limited sequencing technologies available. This could be overcome by the introduction of next generation sequencing (NGS) technology which generates vast amount of phylogenetic data in a short period of time with low costs (Sanz & Kochling 2019). The influence of nitrifying consortia dynamics on the performance of biological contactors has been recently studied using NGS of 16SrRNA genes of nitrifying community (Freeman et al 2020).…”

Section: Analyses Of Nitrifying/denitrifying Key Players In Aquaculturementioning

confidence: 99%

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Preena

Kumar

Singh

2021

Reviews in Aquaculture

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One of the major challenges in any sustainable aquaculture production systems is the accumulation of nitrogenous waste such as ammonia and its biological nitrification products viz nitrite and nitrate. Considering the bio-security issues, amelioration of these wastes without water exchange can be accomplished only by way of establishing in situ nitrification and denitrification through biofilters/ bioreactors activated with nitrifying/denitrifying bioaugmentors. In such systems, coexistence of aerobic denitrifiers, anaerobic ammonia oxidizers (Anammox) and complete ammonia oxidizers (Comammox) together with the autotrophic nitrifiers enhance the coupled nitrification-denitrification. This promotes total nitrogen removal without external carbon supplements or additional anerobic compartment in the system. Various recirculating aquaculture systems (RAS) comprise diverse nitrifying community in biofilters/bioreactors thereby imparting distinctive nitrogen conversion in the system. Meanwhile, the structure and population dynamics of the nitrifying/denitrifying consortia are influenced by the environmental factors forming the decisive factors of the success of the processes. Accordingly, understanding the complexity of nitrifying/denitrifying community composition turns out to be a requirement to facilitate its improvised performance. In this context, the review addresses different biological nitrogen removal systems, significance of nitrification-denitrification in RAS, genetic diversity of the key players in RAS, methods of analysis of their community structure, current application and prospects of the processes in RAS.

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Section: Analyses Of Nitrifying/denitrifying Key Players In Aquaculturementioning

confidence: 99%

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Preena

Kumar

Singh

2021

Reviews in Aquaculture

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“…2017). Additionally, the engineering designs of the reactor, its operation and maintenance, and geographic location have also been suggested to affect the above microbial communities (Sanz and Köchling 2019). Like the other microbial metabolic activities, the SND process also needs certain preferential operational parameters for optimum efficiencies, such as pH, temperature, DO, carbon source, and C:N ratio (Table 2).…”

Section: Bioreactors In the Snd Processesmentioning

confidence: 99%

Role of heterotrophic nitrifiers and aerobic denitrifiers in simultaneous nitrification and denitrification process: a nonconventional nitrogen removal pathway in wastewater treatment

Gupta

Poddar

Nakhate

et al. 2021

Letters Applied Microbiology

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Bacterial species capable of performing both nitrification and denitrification in a single vessel under similar conditions have gained significance in the wastewater treatment scenario considering their unique character of performing the above reactions under heterotrophic and aerobic conditions respectively. Such a novel strategy often referred to as simultaneous nitrification and denitrification (SND) has a tremendous potential in dealing with various wastewaters having low C : N content, considering that the process needs very little or no external carbon source and oxygen supply thus adding to its cost‐effective and environmentally friendly nature. Though like other micro‐organisms, heterotrophic nitrifiers and aerobic denitrifiers convert inorganic or organic nitrogen‐containing substances into harmless dinitrogen gas in the wastewater, their ecophysiological role in the global nitrogen cycle is still not fully understood. Attempts to highlight the role played by the heterotrophic nitrifiers and aerobic denitrifiers in dealing with nitrogen pollution under various environmental operating conditions will help in developing a mechanistic understanding of the SND process to address the issues faced by the traditional methods of aerobic autotrophic nitrification–anaerobic heterotrophic denitrification.

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“…While it is clear that chiral inversion is biologically mediated, , current knowledge on chiral inversion of 2-APAs by microorganisms is still limited, with a few mechanistic studies performed on single bacterial/fungal strains. , In addition, waste and wastewater treatment processes often involve complex microbial communities rather than a single strain, and the relationship between such communities and chiral inversion has not been comprehensively studied. Next-generation sequencing is a useful tool for in-depth investigation of the microbial community in different environmental matrixes, especially in the waste/wastewater treatment context. , 16S rRNA sequencing can also be combined with complementary tools such as functional prediction tool (e.g., PICRUSt2) to provide information about the functional composition of sampled communities . This study aims to provide new insights into chiral inversion under well-controlled anaerobic conditions using pure enantiomers of 11 2-APAs.…”

Section: Introductionmentioning

confidence: 99%

Chiral Inversion of 2-Arylpropionic Acid Enantiomers under Anaerobic Conditions

Nguyễn

McDonald

et al. 2022

Environ. Sci. Technol.

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This work examined the chiral inversion of 2-arylpropionic acids (2-APAs) under anaerobic conditions and the associated microbial community. The anaerobic condition was simulated by two identical anaerobic digesters. Each digester was fed with the substrate containing 11 either pure (R)- or pure (S)-2-APA enantiomers. Chiral inversion was evidenced by the concentration increase of the other enantiomer in the digestate and the changes in the enantiomeric fraction between the two enantiomers. Both digesters showed similar and poor removal of 2-APAs (≤30%, except for naproxen) and diverse chiral inversion behaviors under anaerobic conditions. Four compounds exhibited (S → R) unidirectional inversion [flurbiprofen, ketoprofen, naproxen, and 2-(4-tert-butylphenyl)propionic acid], and the remaining seven compounds showed bidirectional inversion. Several aerobic and facultative anaerobic bacterial genera (Candidatus Microthrix, Rhodococcus, Mycobacterium, Gordonia, and Sphingobium) were identified in both digesters and predicted to harbor the 2-arylpropionyl-CoA epimerase (enzyme involved in chiral inversion) encoding gene. These genera presented at low abundances, <0.5% in the digester dosed with (R)-2-APAs and <0.2% in the digester dosed with (S)-2-APAs. The low abundances of these genera explain the limited extent of chiral inversion observed in this study.

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Next-generation sequencing and waste/wastewater treatment: a comprehensive overview

Cited by 34 publications

References 191 publications

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Role of heterotrophic nitrifiers and aerobic denitrifiers in simultaneous nitrification and denitrification process: a nonconventional nitrogen removal pathway in wastewater treatment

Chiral Inversion of 2-Arylpropionic Acid Enantiomers under Anaerobic Conditions

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