Next-generation sequencing-based Actinobacteria community associated with Callyspongia sp. from Kepulauan Seribu Marine National Park, Jakarta, Indonesia

Abstract

Abstract. Retnowati D, Solihin DD, Ghulamahdi M, Lestari. 2021. Next-generation sequencing-based Actinobacteria community associated with Callyspongia sp. from Kepulauan Seribu Marine National Park, Jakarta, Indonesia. Biodiversitas 22: 3702-3708. Sponges are sessile benthic filter-feeding animals, which harbor numerous microbes. The enormous community and abundance of sponge-associated actinobacteria envisage sponges as hot spots of microbes. The metagenomic approach with Next Generation Sequencing (NGS) technology was used to investigate the actinobacteria community of Callyspongia sp. collected from Kepulauan Seribu Marine National Park, Indonesia. Fourteen actinobacteria families were found associated with the sponge. Among them, Microbacteriaceae was the most dominant family. The community of actinobacteria was higher than that of the culturable method from our previous study. The research showed that a metagenomic approach using NGS technology reveals better information regarding the actinobacteria community associated with Callyspongia sp.

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“…A following study by Retnowati et al (2021) recorded the community structure of Callyspongia sp. from Kepulauan Seribu, Jakarta, Indonesia.…”

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“…According to some research, Proteobacteria included roughly 80-86% of all marine sponge species, including Pione vastifica, Siphonochalina siphonella, Suberea mollis, Eunapius carteri, Corvospongilla lapidosa, Aplysina sp., and Ircinia sp. [11][24] [25].…”

“…Several mathematical formulae, including the Shannon-Weaver and Simpson diversity indices, which are generally employed to evaluate community diversity are utilized to analyze the species diversity index of a community [32,34,35]. NGS detection can provide a more comprehensive view of the bacterial diversity in a sample by identifying the bacteria present without the need to isolate or cultivate individual bacterial colonies because it uses amplified DNA for detection [11,24]. In addition, the identified sponge sequenced was deposited in NCBI GenBank: ON075465.1 To estimate the beta diversity, a PCA was performed on all the datasets using the Minitab 21 version.…”

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“…Metagenomics, a method for acquiring comprehensive genetic information (the entire genome) of microorganisms without culturing based on cell or microbe structure (Behera et al 2020;Retnowati et al 2021), combines statistical meta-analysis and genomics. This system incorporates recent advancements in microbial genomics, PCR amplification, and direct gene cloning from the environment (Kistler et al 2017;Sze and Schloss 2019;Azeem et al 2022).…”