Next-generation sequencing-based QTL mapping for unravelling causative genes associated with melon fruit quality traits

Portnoy, Vitaly; Gonda, Itay; Galpaz, Navot; Tzuri, Galil; Lev, Shery; Kenigswald, Merav; Fei, Z.; Barad, Omer; Harel-Beja, Rotem; Doron‐Faigenboim, Adi; Bar, Einat; Saar, Uzi; Xu, Yong; Lombardi, Nadia; Mao, Linyong; Jiao, Chengzhi; Kol, Guy; Gur, Amit; Fallik, Elazar; Tadmor, Yaakov; Burger, Y.; Schaffer, Arthur A.; Giovannoni, James; Lewinsohn, Efraim; Katzir, Nurit

doi:10.17660/actahortic.2017.1151.3

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Like in other fruits and vegetables, carotenoids are melon's main functional components and micronutrients. Investigation of carotenoid composition, content, and its metabolic pathway in melons should become an important field of melon breeding for quality estimation and nutrition breeding [12]. Carotenoid metabolism is highly dependent on fruit ripening and is related to the metabolism of volatile compounds [13].…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability Analysis Based on Inter-Simple Sequence Repeat And β-Carotene Content Analysis In Melon (Cucumis melo L. ‘GAMA Melon Parfum’)

Wibowo¹,

Rasyid²,

Maharani³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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The 'Gama Melon Parfum' (GMP) melons is a variety of plant breeding resulting from the Faculty of Biology's genetics and breeding laboratory, Universitas Gadjah Mada. GMP melons have a unique phenotypic character of a bitter taste and a strong aroma that has the potential to be used for cosmetics and medicine. Stability and genetic variation test are necessary to ensure the quality control of 'GMP' melons for industrial raw materials. The content of carotenoids is also important to reveal in utilizing metabolites compounds. Phenotypic character analysis was performed by comparing fruit grown conventionally and hydroponically grown on 'GMP' melons. The molecular observation method is genetic variation using the PCR-ISSR method with 5 primer ISSR and comparison with other varieties, namely 'Hikapel', 'Sky Rocket', and 'PI371795'. The data analysis used the UPGMA method, and genetic similarity was estimated using Jaccard Coefficient with MVSP 3.1 program. The method for observing β-carotene content is the UV-Vis spectrophotometric method. The results obtained showed that hydroponically grown 'GMP' melons had a relatively smaller size and faded fruit color. However, phenetically, 'GMP' melons grown both hydroponically and conventionally are in one cluster with a similarity level of 80.9%. Genetic analysis on 'GMP' melons and comparison melons showed a high level of polymorphism of 58.97%. While the results of β-carotene analysis on 'GMP' melons were 140,829 g/100 gr. It can be concluded that GMP melon has a stable genetic character. Cultivation methods and environmental factors cause changes that occur in GMP melons.

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Section: Introductionmentioning

confidence: 99%

Genetic Stability Analysis Based on Inter-Simple Sequence Repeat And β-Carotene Content Analysis In Melon (Cucumis melo L. ‘GAMA Melon Parfum’)

Wibowo¹,

Rasyid²,

Maharani³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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“…There are more than 250 compounds reported in melons that are influenced by genotype and fruit maturity (Lignou et al 2014). Volatile compound biosynthesis involves the pathway between converting fatty acids and amino acids to aldehydes, aldehydes to alcohols, and alcohol compounds to esters (Portnoy et al 2017) In cantaloupensis melon varieties, esters have a significant role as a group of volatile compounds and are likely to be the key that contributes to the formation of the characteristic aroma present in ripe melons (Galaz et al 2013; Galpaz et al 2018). Esters represent 94% of the total 20% volatile compounds in physiologically mature pre-slip fruit (Vallone et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

2020

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Abstract. Wibowo WA, Fatkhurohman MI, Daryono BS. 2020. Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’. Biodiversitas 21: 3041-3046. Melon (Cucumis melo L.) is one of the horticulture commodities that have high economic value and its needs increase continuously. Many new melon cultivars have been assembled to produce a higher quality melon. Melon 'Hikapel' developed by the Laboratory of Genetics and Breeding, Faculty of Biology UGM has distinctive character in the form of a strong aroma. This aroma is a complex mixture of various kinds of volatile compound. One of the main determinant compounds is a volatile ester, synthesized by the alcohol acyl-transferase enzyme encoded by the Cm-AAT1 gene. Characterization of Cm-AAT1 began with isolation of melon rinds to get total RNAs. Synthesis cDNA was conducted with oligo-dT primer, followed by detection of Cm-AAT1 using specific primers. A specific band was sequenced to perform phylogenetic tree. Gene expression from 4 melon cultivars, ‘Hikapel’, ‘Hikadi’, ‘Sun Lady’, and ‘Luna’ analysis was performed using relative quantitative Real-Time PCR. The results of this study showed that Cm-AAT1 owned not only by aromatic cultivars ‘Hikapel’ and ‘Hikadi’, but also owned by non-aromatic cultivars ‘Sun Lady’ and ‘Luna’. Phylogenetic analysis shows a high similarity between Cm-AAT1 on 'Hikapel' and 'Hikadi'. Gene expression analysis on 'Hikapel' increases as the process of fruit ripening during the storage period and it is in contrast to 'Hikadi' at decrease when the fruit began to enter the decay process on day 7th. Expression of Cm-AAT1 on ‘Hikapel’ was higher than ‘Hikadi’ at the peak of fruit maturity.

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Watermelon and melon fruit quality: The genotypic and agro-environmental factors implicated

Kyriacou

Leskovar²,

Colla

et al. 2018

Scientia Horticulturae

125

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Next-generation sequencing-based QTL mapping for unravelling causative genes associated with melon fruit quality traits

Cited by 3 publications

References 29 publications

Genetic Stability Analysis Based on Inter-Simple Sequence Repeat And β-Carotene Content Analysis In Melon (Cucumis melo L. ‘GAMA Melon Parfum’)

Genetic Stability Analysis Based on Inter-Simple Sequence Repeat And β-Carotene Content Analysis In Melon (Cucumis melo L. ‘GAMA Melon Parfum’)

Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

Watermelon and melon fruit quality: The genotypic and agro-environmental factors implicated

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