2018
DOI: 10.1101/340273
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Next-Generation Sequencing Could be a Promising Diagnostic Approach for Pathogen Detection: Pathogenic Analysis of Pediatric Bacterial Meningitis by Next-Generation Sequencing Technology Directly from Cerebrospinal Fluid Specimens

Abstract: BackgroundBacterial meningitis remains one of the major challenges in infectious diseases, leading to sequel in many cases. A prompt diagnosis of the causative microorganism is critical to significantly improve outcome of bacterial meningitis. Although various targeted tests for cerebrospinal fluid (CSF) samples are available, it is a big problem for the identification of etiology of bacterial meningitis.MethodsHere we describe the use of unbiased sequence analyses by next-generation sequencing (NGS) technolog… Show more

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“…Optimisation of point-of-care PCR diagnostics, transcriptomics research to identify RNA signatures associated with ABM and loop-mediated isothermal amplification-based assays 64 may each play a role. However, it is the expanded use of whole genome sequencing in clinical care as it becomes increasingly rapid, accessible, affordable and directly applicable to clinical specimens without the need for culture, that has the greatest potential to transform meningitis diagnostics, particularly prediction of genotypic drug resistance 65 .…”
Section: Future Directionsmentioning
confidence: 99%
“…Optimisation of point-of-care PCR diagnostics, transcriptomics research to identify RNA signatures associated with ABM and loop-mediated isothermal amplification-based assays 64 may each play a role. However, it is the expanded use of whole genome sequencing in clinical care as it becomes increasingly rapid, accessible, affordable and directly applicable to clinical specimens without the need for culture, that has the greatest potential to transform meningitis diagnostics, particularly prediction of genotypic drug resistance 65 .…”
Section: Future Directionsmentioning
confidence: 99%