Next-Generation Sequencing in the Study of Infectious Diseases

Zuckerman, Neta S.; Shulman, Lester M.

doi:10.1007/978-1-0716-2463-0_1090

Cited by 1 publication

(1 citation statement)

References 149 publications

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“…Research on viruses in wild plants offers multiple advantages, such as enhancing our understanding of viral diversity and host-virus interactions, conserving biodiversity, and providing insights into viral evolution [ 9 ]. Next-generation Sequencing (NGS) has revolutionized this field by enabling comprehensive sequencing of all viral genomes in a plant tissue sample [ 10 ]. This high-throughput technology sequences the entire viral genome after isolating its RNA or DNA from the plant tissue, facilitating rapid identification and characterization of known and unknown viruses [ 11 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Metatranscriptome analysis of symptomatic bitter apple plants revealed mixed viral infections with a putative novel polerovirus

Ghorani,

Massumi,

Farhangi

et al. 2024

BMC Genomics

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Background Next-generation Sequencing (NGS) combined with bioinformatic analyses constitutes a powerful approach for identifying and characterizing previously unknown viral genomes. In this study, leaf samples from bitter apple plants (Citrullus colocynthis (L.) Schrad) exhibiting symptoms such as dwarfing, leaf crinkling, and chlorosis were collected from the southern part of Kerman province, Iran. Results Putative infecting viruses were identified through de novo assembly of sequencing reads using various tools, followed by BLAST analysis. Complete genomes for Squash vein yellowing virus (SqVYV), Citrus-associated rhabdovirus (CiaRV), and a novel polerovirus-related strain termed Bitter apple aphid-borne yellows virus (BaABYV) were assembled and characterized. Additionally, a partial genome for Watermelon mosaic virus (WMV) was assembled. The genomic organization of the BaABYV was determined to be 5’-ORF0-ORF1-ORF1,2-ORF3a-ORF3-ORF3,5-ORF4-3’. Amino acid sequence identities for inferred proteins (P0 and P1, P1,2) with known poleroviruses were found to be the 90% species delineation limit, implying that BaABYV should be considered a new member of the genus Polerovirus. Recombination events were observed in the BaABYV and WMV strains; such events were not found in the CiaRV strain. Conclusions Molecular evidence from this study suggests that C. colocynthis is a reservoir host of several plant viruses. Among them, BaABYV is proposed as a new member of the genus Polerovirus. Furthermore, the CiaRV strain has been reported for the first time from Iran.

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