Next-generation sequencing metabarcoding assays reveal diverse bacterial vector-borne pathogens of Mongolian dogs

Davitt, Cassandra; Huggins, Lucas G.; Pfeffer, Martin; Batchimeg, Lkhagvasuren; Jones, Malcolm; Battur, Banzragch; Wiethoelter, Anke K.; Traub, Rebecca

doi:10.1016/j.crpvbd.2024.100173

Cited by 3 publications

(1 citation statement)

References 70 publications

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“…This approach shed light into tick-borne illnesses. Davitt et al also underscore the importance of pathogen surveillance studies in under-researched regions and reinforce the efficacy of NGS as an explorative diagnostic tool [ 78 ].…”

Section: Infectious Diseases In Animalsmentioning

confidence: 99%

Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals

Domrazek,

Jurka

2024

Animals

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Next-Generation Sequencing (NGS) techniques have revolutionized veterinary medicine for cats and dogs, offering insights across various domains. In veterinary parasitology, NGS enables comprehensive profiling of parasite populations, aiding in understanding transmission dynamics and drug resistance mechanisms. In infectious diseases, NGS facilitates rapid pathogen identification, characterization of virulence factors, and tracking of outbreaks. Moreover, NGS sheds light on metabolic processes by elucidating gene expression patterns and metabolic pathways, essential for diagnosing metabolic disorders and designing tailored treatments. In autoimmune diseases, NGS helps identify genetic predispositions and molecular mechanisms underlying immune dysregulation. Veterinary oncology benefits from NGS through personalized tumor profiling, mutation analysis, and identification of therapeutic targets, fostering precision medicine approaches. Additionally, NGS plays a pivotal role in veterinary genetics, unraveling the genetic basis of inherited diseases and facilitating breeding programs for healthier animals. Physiological investigations leverage NGS to explore complex biological systems, unraveling gene–environment interactions and molecular pathways governing health and disease. Application of NGS in treatment planning enhances precision and efficacy by enabling personalized therapeutic strategies tailored to individual animals and their diseases, ultimately advancing veterinary care for companion animals.

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Section: Infectious Diseases In Animalsmentioning

confidence: 99%

Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals

Domrazek,

Jurka

2024

Animals

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Bartonella rochalimae in a flea collected from a Mephitis macroura in Sonora Mexico

Fernández-González,

Herrera-Mares,

Ramírez-Corona

et al. 2024

Acta Parasit.

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Purpose At least thirty species of wild carnivores have been recorded harboring Bartonella, and one of the most common pathogenic species infecting them is Bartonella rochalimae, which can cause endocarditis in humans and dogs. This bacterium can infect various mammals including wild carnivores, as well as ectoparasitic vectors such as fleas and ticks. Here we report the presence of B. rochalimae, in a Pulex simulans flea collected from a Mephitis macroura skunk in the municipality of Santa Cruz in Sonora, Mexico. Methods Fleas were collected from a M. macroura in Sonora, Mexico, in October 2019. They were identified to species level and subsequently tested for the presence of Bartonella using molecular tools including conventional PCR, sequencing, and phylogenetic analysis. Results A total of 10 P. simulans fleas (one male, nine females) were collected from the M. macroura skunk. The PCR and phylogenetic analysis indicated a prevalence of 10% (1/10) and a sequence clustered with the clade of B. rochalimae. Conclusions We confirmed the presence of B. rochalimae in a P. simulans flea collected from a M. macroura skunk in the area of Santa Cruz, Sonora, Mexico. Based on our results and previous studies in northern Mexico, which are consistent, it is necessary to continue monitoring Bartonella in M. macroura skunks and their fleas, since they could be important reservoirs of this bacterium in northern Mexico.

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Metabarcoding study of potential pathogens and zoonotic risks associated with dog feces in Seoul, South Korea

Liyanagama,

Oh,

Choi

et al. 2024

PLoS Negl Trop Dis

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Background A significant portion of South Korea’s population, approximately a quarter, owns pets, with dogs being the most popular choice among them. However, studies analyzing the fecal organism communities of dogs in South Korea are lacking, and limited efforts have been exerted to identify pathogens with potential zoonotic implications. Therefore, this study aimed to investigate potential pathogens using metabarcoding analysis and evaluate the risk of zoonotic diseases in dog feces in Seoul, South Korea. Methodology Fecal samples were collected from both pet and stray dogs in the Mapo district of Seoul. Next-generation sequencing (NGS) was utilized, employing 16S rRNA amplicon sequencing to identify prokaryotic pathogens, and 18S rRNA amplicon sequencing for eukaryotic pathogens. The data obtained from the QIIME2 pipeline were subjected to various statistical analyses to identify different putative pathogens and their compositions. Principal findings Significant variations in microbiota composition were found between stray and pet dogs, and putative prokaryotic and eukaryotic pathogens were identified. The most prevalent putative bacterial pathogens were Fusobacterium, Helicobacter, and Campylobacter. The most prevalent putative eukaryotic pathogens were Giardia, Pentatrichomonas, and Cystoisospora. Interestingly, Campylobacter, Giardia, and Pentatrichomonas were found to be significantly more prevalent in stray dogs than in pet dogs. The variation in the prevalence of potential pathogens in dog feces could be attributed to environmental factors, including dietary variances and interactions with wildlife, particularly in stray dogs. These factors likely contributed to the observed differences in pathogen occurrence between stray and pet dogs. Conclusions/Significance This study offers valuable insights into the zoonotic risks associated with dog populations residing in diverse environments. By identifying and characterizing putative pathogens in dog feces, this research provides essential information on the impact of habitat on dog-associated pathogens, highlighting the importance of public health planning and zoonotic risk management.

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Next-generation sequencing metabarcoding assays reveal diverse bacterial vector-borne pathogens of Mongolian dogs

Cited by 3 publications

References 70 publications

Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals

Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals

Bartonella rochalimae in a flea collected from a Mephitis macroura in Sonora Mexico

Metabarcoding study of potential pathogens and zoonotic risks associated with dog feces in Seoul, South Korea

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