Next generation sequencing of oral microbiota in Type 2 diabetes mellitus prior to and after neem stick usage and correlation with serum monocyte chemoattractant-1

Anbalagan, Rubini; Srikanth, Padma; Mani, Monika; Barani, Ramya; Seshadri, Krishna G; Janarthanan, R.

doi:10.1016/j.diabres.2017.06.009

Cited by 33 publications

(24 citation statements)

References 13 publications

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“…In general, Firmicutes, Bacteroidetes, and Proteobacteria were the most abundantly present bacterial phyla in the salivary microbiome of our study samples. Previous studies also showed that these bacterial phyla are most abundantly present in our mucosal communities and are associated with host metabolic rate and energy homeostasis [42,43]. However, there are also contradictory findings in the literature about microbial phylogenetic association with T2DM.…”

Section: Discussionmentioning

confidence: 88%

“…Similarly, Long et al (2017) found that although Firmicutes are the most abundantly present bacteria in oral microbiome, bacterial taxa found in the phylum Actinobacteria are associated with the risk of T2DM development [18]. In contrast, Anbalagan et al [43] observed that the oral microbiome of T2DM patients was not different from healthy controls. These controversies in observations could be attributed to different study designs and sampling methods.…”

Section: Discussionmentioning

confidence: 99%

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Profiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar

et al. 2019

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The present study is designed to compare demographic characteristics, plasma biochemistry, and the oral microbiome in obese (N = 37) and lean control (N = 36) subjects enrolled at Qatar Biobank, Qatar. Plasma hormones, enzymes, and lipid profiles were analyzed at Hamad Medical Cooperation Diagnostic Laboratory. Saliva microbiome characterization was carried out by 16S rRNA amplicon sequencing using Illumina MiSeq platform. Obese subjects had higher testosterone and sex hormone-binding globulin (SHBG) concentrations compared to the control group. A negative association between BMI and testosterone (p < 0.001, r = −0.64) and SHBG (p < 0.001, r = −0.34) was observed. Irrespective of the study groups, the oral microbiome was predominantly occupied by Streptococcus, Prevotella, and Veillonella species. A generalized linear model revealed that the Firmicutes/Bacteroidetes ratio (2.25 ± 1.83 vs. 1.76 ± 0.58; corrected p-value = 0.04) was higher, and phylum Fusobacteria concentration (4.5 ± 3.0 vs. 6.2 ± 4.3; corrected p-value = 0.05) was low in the obese group compared with the control group. However, no differences in microbiome diversity were observed between the two groups as evaluated by alpha (Kruskal–Wallis p ≥ 0.78) and beta (PERMANOVA p = 0.37) diversity indexes. Certain bacterial phyla (Acidobacteria, Bacteroidetes, Fusobacteria, Proteobacteria, Spirochaetes, and Firmicutes/Bacteroidetes) were positively associated (p = 0.05, r ≤ +0.5) with estradiol, fast food consumption, creatinine, breastfed during infancy, triglycerides, and thyroid-stimulating hormone concentrations. In conclusion, no differences in oral microbiome diversity were observed between the studied groups. However, the Firmicutes/Bacteroidetes ratio, a recognized obesogenic microbiome trait, was higher in the obese subjects. Further studies are warranted to confirm these findings in a larger cohort.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Profiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar

et al. 2019

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Section: Resultsmentioning

confidence: 98%

“…Anbalagan et al [52] also reported that MCP-1 chemokine in the oral cavity has a special diagnostic significance in patients with type 2 diabetes mellitus. In particular, they emphasized that it is directly associated with the bacterial load in the oral cavity, since a reduction in the bacterial load due to therapeutic and prophylactic measures is also accompanied by a decline in the salivary MCP-1 level [52]. However, paradoxical results were obtained in our study: all other conditions being equal and no treatment measures being performed, the MCP-1 level in the oral cavity of patients with chronic periodontitis associated with type 2 diabetes mellitus was lower than that of patients with chronic periodontitis without the somatic comorbidity.…”

Section: Resultsmentioning

confidence: 98%

“Shielding” of Cytokine Induction by the Periodontal Microbiome in Patients with Periodontitis Associated with Type 2 Diabetes Mellitus

et al. 2019

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Periodontal diseases, especially those with polymicrobial etiology, are often associated with type 2 diabetes mellitus, proceeding more severely and affecting the course of diabetes mellitus. Recently, this feature has been associated with the ability of periodontopathogen microflora to cause not only a local infectious process in the oral cavity, but also to interact with the human immune system and induce various systemic effects. We investigated changes in the salivary cytokine profile of patients with chronic periodontitis, associated and not associated with type 2 diabetes mellitus. We observed a statistically significant decrease of MCP-1/CCL2, GM-CSF, IL-5, IL-6, and IFN- in the saliva of patients with chronic periodontitis associated with type 2 diabetes mellitus in comparison with patients with chronic periodontitis only. All of these cytokines are associated with macrophage activation. These data are an important contribution to the elucidation of the mechanism of periodontopathogens involvement in the manifestation of the systemic effects of type 2 diabetes.

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“…(5)(6)(7) Several studies have documented the oral microbiota in diabetic patients as well as other patient populations. (8)(9)(10) Sharma and coworkers reported that both Gram positive and Gram negative bacteria are fairly involved in dental diseases and that the prevalence of bacteria increases with severity of disease. In another related study, it was reported that periodontal pathogens were different in diabetic patients and non-diabetic controls as well as in aggressive and chronic periodontitis.…”

Section: Introductionmentioning

confidence: 99%

Probiotics Effect on the Gingiva of Uncontrolled Diabetic Albino Rats

Kamel¹,

ElKady²

2018

Egyptian Dental Journal

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Objective: This study was designed to evaluate the effect of using L.Reuteri containing tablets, on the gingiva of uncontrolled diabetic rats both histologically and ultrastructurally. Material and Methods:Thirty adult male albino rats were equally divided into three groups, Group I served as control, Group II: rats were subjected to induction of diabetes mellitus using streptozocin, Group III: rats treated as group II animals and on the next day they were fed daily a probiotic tablet Prodentis containing L. reuteri for 14 days. At the end of the experiment, histological and ultrastructural examination was performed on the gingiva. Results:Rats of group II revealed inflammatory gingiva with loss of normal structure, while that of group III revealed a somewhat normal gingiva in comparison to group II.Conclusion: L.reuteri containg tablets can inhibit gingival inflammation associated with diabetics through its antibacterial and autoinflammatory properties.

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Next generation sequencing of oral microbiota in Type 2 diabetes mellitus prior to and after neem stick usage and correlation with serum monocyte chemoattractant-1

Cited by 33 publications

References 13 publications

Profiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar

Profiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar

“Shielding” of Cytokine Induction by the Periodontal Microbiome in Patients with Periodontitis Associated with Type 2 Diabetes Mellitus

Probiotics Effect on the Gingiva of Uncontrolled Diabetic Albino Rats

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