NF-KappaB expression correlates with apoptosis and angiogenesis in clear cell renal cell carcinoma tissues

Meteoğlu, İbrahim; Erdoğdu, İbrahim Halil; Meydan, Nezih; Erkuş, Muhan; Barutça, Sabri

doi:10.1186/1756-9966-27-53

Cited by 75 publications

(66 citation statements)

References 50 publications

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“…NF-kB activation is well known to play a role in the regulation of these angiogenesisinducing products (31). Although VEGF is a potent angiogenic factor, the mere inhibition of VEGF with bevacizumab has failed to demonstrate substantial prolongation of OS in patients with ovarian cancer, indicating that there are several alternative pathways of angiogenesis other than the VEGF pathway.…”

Section: Discussionmentioning

confidence: 99%

IKKβ Regulates VEGF Expression and Is a Potential Therapeutic Target for Ovarian Cancer as an Antiangiogenic Treatment

Kinose

Sawada

Makino

et al. 2015

Molecular Cancer Therapeutics

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The prolongation of progression-free survival (PFS) in patients with advanced ovarian cancer by antiangiogenic therapy has been shown in several clinical trials. However, although an anti-VEGF antibody (bevacizumab) is the only option currently available, its efficacy is limited and it is not cost effective for use in all patients. Therefore, the development of a novel antiangiogenic drug, especially composed of smallmolecule compounds, could be a powerful armament for ovarian cancer treatment. As NF-kB signaling has the potential to regulate VEGF expression, we determined to identify whether VEGF expression is associated with NF-kB activation and to investigate the possibility of a novel IKKb inhibitor, IMD-0354 (IMMD Inc.), as an antiangiogenic drug. Tissue microarrays from 94 ovarian cancer tissues were constructed and immunohistochemical analyses performed. We revealed that IKK phosphorylation is an independent prognostic factor (PFS: 26

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Section: Discussionmentioning

confidence: 99%

IKKβ Regulates VEGF Expression and Is a Potential Therapeutic Target for Ovarian Cancer as an Antiangiogenic Treatment

Kinose

Sawada

Makino

et al. 2015

Molecular Cancer Therapeutics

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“…The ratio of Bax to Bcl-X L is a crucial factor which regulates susceptibility of cells to apoptosis (Kroemer, 1997;Gobe et al, 2001). including those in apoptosis, cell survival, and inflammation (Meteoglu et al, 2008;Morais et al, 2011). TNF-α acts as an inflammatory mediator and plays a significant role in the immune response in chronic kidney disease (Frigo et al, 2005;Lee et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Protection against oxidative stress-induced apoptosis in kidney epithelium by Angelica and Astragalus

Shahzad

Small

Morais

et al. 2016

Journal of Ethnopharmacology

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Ethnopharmacological relevance: Astragalus membranaceus either alone or in combination withAngelica sinensis has been used traditionally for kidney disease in East Asia and China for thousands of years. Previous studies using in vivo animal models have shown the benefits of these medicinal herbs in kidney diseases that involve oxidative stress. However, the mechanisms by which these medicinal herbs protect kidney cells remain largely unknown.Aim of the study: To investigate the mechanisms by which ethanol, methanol and aqueous crude extracts of roots of A. membranaceus and A. sinensis afford protection to human kidney proximal tubular epithelial cells, using an in vitro model of oxidative stress. Materials and Methods:Ethanol, methanol and aqueous extracts of roots of A. membranaceus and A. sinensis were prepared by a three-solvent sequential process. HK2 human kidney proximal tubular epithelial cells were treated with H 2 O 2 alone (0.5 mM) or in combination with different concentrations of extracts. Cell mitosis and death (microscopy) and cell viability (MTT assay) were compared. Western immunoblot was used to study expression of apoptosis-related proteins (pro-apoptotic Bax andanti-apoptotic Bcl-X L ), and cell survival (NFκB subunits p65 and p50), pro-inflammatory (TNF-α) and protective (TGFβ1) proteins.Results: H 2 O 2 -induced oxidative stress significantly increased apoptosis and reduced cell survival; upregulated pro-apoptotic and down-regulated Bcl-X L ; increased NFκB (p65, p50); increased TNFα; and decreased TGFβ1. All changes indicated kidney damage and dysfunction.All were modulated by all extracts of both plant species, except for NFκB which was only modulated by extracts of A. membranaceus. Conclusions:In conclusion, in a model of oxidative stress that might occur after nephrotoxicity, the plant extracts were protective via anti-apoptotic and anti-inflammatory mechanisms.3

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“…Altered regulation of NF-kB is observed in many cancers. This transcription factor modulates the activation of genes related to cell proliferation, angiogenesis, metastasis, tumor promotion, inflammation, and apoptosis suppression (Watanabe et al, 2000;Lind et al, 2001;Cilloni et al, 2008;Meteoglu et al, 2008). Inhibition of the NF-kB signaling pathway is therefore a promising target for chemopreventive and chemotherapeutic approaches.…”

Section: Introductionmentioning

confidence: 99%

Oral Concentrated Grape Juice Suppresses Expression of NF-kappa B, TNF-α and iNOS in Experimentally Induced Colorectal Carcinogenesis in Wistar Rats

Campanholo

Silva²,

Silva³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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The aim of this study was to evaluate the effects of grape juice on colon carcinogenesis induced by azoxymethane (AOM) and expression of NF-kB, iNOS and TNF-α. Methods: Forty male Wistar rats were divided into 7 groups: G1, control; G2, 15 mg/kg AOM; G3, 1% grape juice 2 weeks before AOM; G4, 2% grape juice 2 weeks before AOM; G5, 1% grape juice 4 weeks after AOM; G6, 2% grape juice 4 weeks after AOM; G7, 2% grape juice without AOM. Histological changes and aberrant crypt foci (ACF) were studied, while RNA expression of NFkB, TNF-and iNOS was evaluated by qPCR. Results: The number of ACF was higher in G2, and G4 presented a smaller number of crypts per focus than G5 (p=0.009) and G6. Small ACF (1-3) were more frequent in G4 compared to G2, G5 and G6 (p=0.009, p=0.009 and p=0.041, respectively). RNA expression of NF-kB was lower in G3 and G4 compared to G2 (p=0.004 and p=0.002, respectively). A positive correlation was observed between TNF-α and NF-kB gene expression (p=0.002). In conclusion, the administration of 2% grape juice before AOM reduced the crypt multiplicity, attenuating carcinogenesis. Lower expression of NF-kB was observed in animals exposed to grape juice for a longer period of time, regardless of concentration.

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NF-KappaB expression correlates with apoptosis and angiogenesis in clear cell renal cell carcinoma tissues

Cited by 75 publications

References 50 publications

IKKβ Regulates VEGF Expression and Is a Potential Therapeutic Target for Ovarian Cancer as an Antiangiogenic Treatment

IKKβ Regulates VEGF Expression and Is a Potential Therapeutic Target for Ovarian Cancer as an Antiangiogenic Treatment

Protection against oxidative stress-induced apoptosis in kidney epithelium by Angelica and Astragalus

Oral Concentrated Grape Juice Suppresses Expression of NF-kappa B, TNF-α and iNOS in Experimentally Induced Colorectal Carcinogenesis in Wistar Rats

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