NF-κB–Dependent Snail Expression Promotes Epithelial–Mesenchymal Transition in Mastitis

Liu, Haokun; Zhao, Ying; Wu, Yanfang; Yan, Yutong; Zhao, Xiaoe; Wei, Qiang; Ma, Baohua

doi:10.3390/ani11123422

Cited by 2 publications

(1 citation statement)

References 51 publications

(52 reference statements)

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“…After disinfecting the teat of goat mammary three times with 75% alcohol and discarding the first three handfuls of milk, milk was sampled in the morning at one time; about 40 mL of milk was sampled with a sterile tube (50 mL), transported at low temperature for somatic cell staining or immediately transported with dry ice for detection of S100A7 concentration. Twelve goat mammary tissues were sampled from the Fuping slaughterhouse in ShaanXi Province, China, which were fixed in 4% paraformaldehyde to perform paraffin secretions, or immediately frozen in liquid nitrogen for RNA extraction [39].…”

Section: Milk Sample and Goat Mammary Tissue Samplementioning

confidence: 99%

The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats

Yan,

Zhu,

Liu

et al. 2023

Veterinary Sciences

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S100A7 is an inflammation-related protein and plays an essential role in host defenses, yet there is little research about the relationship between mastitis and S100A7 expression in dairy goats. Here, according to the clinical diagnosis of udders, SCC, and bacteriological culture (BC) of milk, 84 dairy goats were grouped into healthy goats (n = 25), subclinical mastitis goats (n = 36), and clinical mastitis goats (n = 23). The S100A7 concentration in subclinical mastitis goats was significantly upregulated than in healthy dairy goats (p = 0.0056) and had a limited change with clinical mastitis dairy goats (p = 0.8222). The relationship between log10 SCC and S100A7 concentration in milk was positive and R = 0.05249; the regression equation was Y = 0.1446 × X + 12.54. According to the three groups, the log10 SCC and S100A7 were analyzed using the receiver operating characteristics (ROC) curve; in subclinical mastitis goats, the area under the ROC curve (AUC) of log10 SCC was 0.9222 and p < 0.0001, and the AUC of S100A7 concentration was 0.7317 and p = 0.0022, respectively; in clinical mastitis goats, the AUC of log10 SCC was 0.9678 and p < 0.0001, and the AUC of S100A7 concentration was 0.5487 and p = 0.5634, respectively. In healthy goats, S100A7 was expressed weakly in the alveolus of the mammary gland of healthy goats while expressed densely in the collapsed alveolus of mastitis goats. Moreover, S100A7 expression increased significantly in mastitis goats than in healthy dairy goats. In this research, results showed the effects of mastitis on the S100A7 expression in the mammary gland and S100A7 concentration in milk and the limited relationship between SCC and mastitis, which provided a new insight into S100A7’s role in the host defenses of dairy goats.

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Section: Milk Sample and Goat Mammary Tissue Samplementioning

confidence: 99%

The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats

Yan,

Zhu,

Liu

et al. 2023

Veterinary Sciences

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Epithelial to mesenchymal transition in mammary gland tissue fibrosis and insights into drug therapeutics

Ahmad

Bhat

Wali

et al. 2023

PeerJ

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Background The epithelial-mesenchymal transition (EMT) is a multi-step morphogenetic process in which epithelial cells lose their epithelial properties and gain mesenchymal characteristics. The process of EMT has been shown to mediate mammary gland fibrosis. Understanding how mesenchymal cells emerge from an epithelial default state will aid in unravelling the mechanisms that control fibrosis and, ultimately, in identifying therapeutic targets to alleviate fibrosis. Methods The effects of EGF and high glucose (HG) on EMT in mammary epithelial cells, MCF10A and GMECs, as well as their pathogenic role, were studied. In-silico analysis was used to find interacting partners and protein-chemical/drug molecule interactions. Results On treatment with EGF and/or HG, qPCR analysis showed a significant increase in the gene expression of EMT markers and downstream signalling genes. The expression of these genes was reduced on treatment with EGF+HG combination in both cell lines. The protein expression of COL1A1 increased as compared to the control in cells treated with EGF or HG alone, but when the cells were treated with EGF and HG together, the protein expression of COL1A1 decreased. ROS levels and cell death increased in cells treated with EGF and HG alone, whereas cells treated with EGF and HG together showed a decrease in ROS production and apoptosis. In-silico analysis of protein-protein interactions suggest the possible role of MAPK1, actin alpha 2 (ACTA2), COL1A1, and NFκB1 in regulating TGFβ1, ubiquitin C (UBC), specificity protein 1 (SP1) and E1A binding protein P300 (EP300). Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment suggests advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE) signalling pathway, relaxin signalling pathway and extra cellular matrix (ECM) receptor interactions underlying fibrosis mechanism. Conclusion This study demonstrates that EGF and HG induce EMT in mammary epithelial cells and may also have a role in fibrosis.

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NF-κB–Dependent Snail Expression Promotes Epithelial–Mesenchymal Transition in Mastitis

Cited by 2 publications

References 51 publications

The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats

The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats

Epithelial to mesenchymal transition in mammary gland tissue fibrosis and insights into drug therapeutics

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