NF-κB is a target of AKT in anti-apoptotic PDGF signalling

Romashkova, J.; Makarov, Sergei S.

doi:10.1038/43474

Cited by 1,735 publications

(1,358 citation statements)

References 30 publications

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“…In contrast to a previous report (Mayo et al, 1997), induction of oncogenic HRas protein had little e ect on cell viability of RIE(iRas)/DN-IkBa cells. Moreover, consistent with the ®ndings that Ras promotes cell survival rather than cell death in epithelial cells (Cardone et al, 1998;Downward, 1998;Kau man-Zeh et al, 1997;Khwaja et al, 1997;Romashkova and Makarov, 1999), H-Ras induction signi®cantly prevented cell death of RIE(iRas)/DN-IkBa cells upon TNF-a treatment as determined by colonigenic assay (Figure 4c). These observations suggest that reduction in cell number of RIE(iRas)/DN-IkBa cells over that of control cells upon Ras induction is due to growth suppression rather than increased cell death.…”

Section: Inactivation Of Nf-kb Leads To Growth Inhibition and Reducedsupporting

confidence: 86%

NF-κB is required for H-ras oncogene induced abnormal cell proliferation and tumorigenesis

Zhang

et al. 2000

Oncogene

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Section: Inactivation Of Nf-kb Leads To Growth Inhibition and Reducedsupporting

confidence: 86%

NF-κB is required for H-ras oncogene induced abnormal cell proliferation and tumorigenesis

Zhang

et al. 2000

Oncogene

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“…Among other regulatory cascades is AKT/PKB, which was implicated in the regulation of IkB (Romashkova and Makarov, 1999;Ozes et al, 1999). Analysis of PKB/AKT phosphorylation in the melanoma cells studied here did not reveal changes after UV-treatment.…”

Section: Discussionmentioning

confidence: 99%

p38 protects human melanoma cells from UV-induced apoptosis through down-regulation of NF-κB activity and Fas expression

2000

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Identifying mechanisms that underlie the resistance of human melanoma to radiation and chemotherapy is expected to assist in developing new strategies for the treatment of this tumor type. We recently demonstrated that through up-regulation of TNFa, ATF2 increases the resistance of late stage melanoma cells to apoptosis induced by UV-irradiation. In elucidating the role of ATF2 kinases, we now demonstrate that ASK1/MKK6/ p38 elicits suppression of Fas expression. ASK1/p38 downregulates the expression of a Fas via NF-kB/SP1 site on the Fas promoter. Deletion or mutation of NFkB/SP1 within the Fas promoter abrogates p38 e ect. ASK1/p38 silences the Fas promoter by inhibition of IkBa phosphorylation ± thereby limiting NF-kB activity. Forced expression of a dominant negative form of p38 (p38-ASP) or treatment with p38 pharmacological inhibitor, SB203580, increases NF-kB activity, Fas expression and the levels of UVC-induced apoptosis in late stage melanoma cells. Inhibition of p38 activity also restored NF-kB activity and Fas expression in earlyphase melanoma cells, suggesting that p38 elicited suppression of Fas expression is not restricted to late phase melanoma. Identifying p38-mediated down-regulation of Fas expression illustrates a novel regulatory pathway by which ASK1/MKK6/p38 alters the degree and nature of the UV-induced apoptosis of melanoma cells.

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“…Because these neurohumoral factors prevent neural apoptosis by the activation of the PI3K-Akt/NF-κB signalling pathway in vitro [20][21][22][23] , the neuroprotective effects through NSPC transplantation were examined. Histopathological examinations at 7 DAT revealed fewer TUNEL-positive cells and more spared myelin in the NSPC-transplanted group (Fig.…”

Section: Cell Viability and Functional Improvementmentioning

confidence: 99%

Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells

et al. 2012

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neural stem/progenitor cell (nsPC) transplantation is a promising treatment for various neurodegenerative disorders including spinal cord injury, however, no direct analysis has ever been performed on their in vivo profile after transplantation. Here we combined bioimaging, flow-cytometric isolation and ultra-high-throughput RnA sequencing to evaluate the cellular properties of engrafted nsPCs. The acutely transplanted nsPCs had beneficial effects on spinal cord injury, particularly neuroprotection and neurohumoral secretion, whereas their in situ secretory activity differed significantly from that predicted in vitro. The RnA-sequencing of engrafted nsPCs revealed dynamic expression/splicing changes in various genes involved in cellular functions and tumour development depending on graft environments. notably, in the pathological environment, overall transcriptional activity, external signal transduction and neural differentiation of engrafted nsPCs were significantly suppressed. These results highlight the vulnerability of engrafted nsPCs to environmental force, while emphasizing the importance of in situ analysis in advancing the efficacy and safety of stem cell-based therapies.

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NF-κB is a target of AKT in anti-apoptotic PDGF signalling

Cited by 1,735 publications

References 30 publications

NF-κB is required for H-ras oncogene induced abnormal cell proliferation and tumorigenesis

NF-κB is required for H-ras oncogene induced abnormal cell proliferation and tumorigenesis

p38 protects human melanoma cells from UV-induced apoptosis through down-regulation of NF-κB activity and Fas expression

Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells

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