NF-κB-mediated anti-inflammatory activity of the sesquiterpene lactone 7-hydroxyfrullanolide

NPS31807, a Standardized Extract from <i>Sphaeranthus indicus</i>, Inhibits Inflammatory, Migratory and Proliferative Activity in Keratinocytes and Immune Cells

“…This could mean that the anti-inflammatory activity of NPS31807 could be in-part due to the content of 7-HF. Indeed, the primary mechanism of 7-HF was found to be mediated through NFκB [24] in human peripheral blood monocytes. Similarly, NPS31807 reduced nuclear translocation of p65/RELA in THP-1 cells (Figure 2(f)) and reporter expression of NFκB and AP-1 as revealed by plasmid transfection assays (Figure 2(e)).…”

“…Nuclear translocation of p65/RELA was quantified using cell based imaging studies in macrophage cells as described previously [24]. THP-1 cells were seeded into 96well MTPs (Nunc, Roskilde, Denmark) at a density of 1 × 10 4 cells/well, treated with varying concentrations of NPS31807 for 15 minutes preceding stimulation with LPS (1 μg/mL) for 30 minutes (p65/REL).…”

“…THP-1 cells were seeded into 96well MTPs (Nunc, Roskilde, Denmark) at a density of 1 × 10 4 cells/well, treated with varying concentrations of NPS31807 for 15 minutes preceding stimulation with LPS (1 μg/mL) for 30 minutes (p65/REL). The plates were processed and quantitative imaging was performed using the high content imaging systems from Cellomics ® (Thermo Scientific, USA) as described previously in detail [24]. The intensity values for treatment groups were normalized to values from LPS stimulation which was considered as 100%.…”

Mugwort (<i>Artemisia vulgaris</i>, <i>Artemisia douglasiana</i>, <i>Artemisia argyi</i>) in the Treatment of Menopause, Premenstrual Syndrome, Dysmenorrhea and Attention Deficit Hyperactivity Disorder

“…In other words, it penetrates the skin after topical administration and relieves inflammation. The mechanism of anti-inflammatory action of a sesquiterpene, 7-hydroxyfrullanolide, appears to be inhibition of an NF-κB pathway [16]. IKKβ phosphorylation was shown to be inhibited by 7-hydroxyfrullanolide, which resulted in inhibition of NF-κB translocation into the nucleus.…”

“…Several sesquiterpenes inhibit the production of inflammatory cytokines and adipokines. 7-Hydroxyfrullanolide inhibits the production of inflammatory adhesion proteins such as ICAM-1, VCAM-1 and E-selectin [16]. This inhibits monocyte induced inflammation.…”