NF1 gene loss of heterozygosity and expression analysis in sporadic colon cancer

Čačev, Tamara; Radošević, Senka; Spaventi, Radan; Pavelić, Krešimir; Kapitanović, Sanja

doi:10.1136/gut.2004.053348

Cited by 41 publications

(29 citation statements)

References 25 publications

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“…Oligonucleotides capable of selective amplification of each variant were used to initiate realtime qPCRs. The copy number of mRNA transcripts was normalized to the expression of the housekeeping gene ARPP0, also known as ribosomal protein large P0, which is often used as an endogenous control in research on prostate and colon cancer (18,19). The transcript encoding IBABP-L was found at similar 1 http://www.ncbi.nlm.nih.gov/ Figure 1.…”

Section: Resultsmentioning

confidence: 99%

“…In the first step, cDNA was synthesized from total RNA. For each sample, RNA (2.0 Ag) was reverse transcribed using SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen) in a 20 AL final reaction volume containing 10 mmol/L deoxynucleotide triphosphate mix (1.0 AL), 0.5 Ag/mL oligo(dT) [12][13][14][15][16][17][18] (in exon 7); the primer set of ARPP0 is 5 ¶-CAAGACTGGAGACAAAGTGG-3 ¶ and 5 ¶-AATCTGCAGA-CAGACACTGG-3 ¶. All primers were designed using PrimerSelect (DNAStar) and synthesized by Integrated DNA Technologies, Inc.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

2007

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Ileal bile acid binding protein (IBABP) is the only cytosolic protein known to bind and transport bile acids. Because IBABP is reportedly up-regulated in colorectal cancer, it has been suggested as a link between bile acids and the risk of colorectal cancer. However, in this study, we show that IBABP is not up-regulated. Rather, a novel transcript of the IBABP gene, which encodes an additional 49 NH 2 -terminal amino acid residues, is up-regulated in colorectal cancer (P < 0.001). The novel transcript, called IBABP-L, is also distinct from IBABP because its transcription is controlled by nuclear factor-KB (NF-KB) rather than by the farnesoid X receptor. Most significantly, IBABP-L is necessary for the survival of HCT116 colon cancer cells in the presence of physiologic levels of the secondary bile acid deoxycholate. Collectively, the studies point toward a unique bile acid response pathway involving NF-KB and IBABP-L that could be useful for diagnosis and could potentially be targeted for therapeutic benefit.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

2007

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“…It has been proposed that the loss of heterozygosity of the NF1 gene that is found in some sporadic colorectal cancers may influence regulation of the Ras/MAPK signalling pathway during the development and progression of colorectal cancer. 12 …”

Section: Discussionmentioning

confidence: 97%

Colonic Vascular Anomalies and Colon Cancer in Neurofibromatosis: Report of a Case

Wood¹,

Longman²,

Rooney³

et al. 2008

Diseases of the Colon &Amp; Rectum

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We report a case of neurofibromatosis with synchronous adenocarcinomas in the sigmoid and transverse colon. There was widespread intimal proliferation in arteries in the region of the tumors and also in the cecum. Such vascular lesions are associated with von Recklinghausen's disease. The cecal lesion produced mural thickening visible on computed tomography. This case supports a possible genetic link between neurofibromatosis and adenocarcinoma of the colon.

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“…The NF1 gene protein, neurofibromin, acts as a tumor suppressor by turning the active form of Ras into an inactive form. This molecular switch has an important role in the control of the cell cycle and differentiation, and the changes in Ras activity are present in many different cancers including colon cancer (Cacev et al, 2005). EVI2B is a gene lying in an intron of the neurofibromatosis type 1 (NF1) gene involved in differentiation of melanocytes and keratinocytes and is overexpressed in cells derived from NF1 neurofibromas (Cawthon et al, 1991;Kaufmann et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

EVI2B,ATP2A2,S100B,TM4SF3, andOLFM4As Potential Prognostic Markers for Postoperative Taiwanese Colorectal Cancer Patients

Wang

Tok

Chang

et al. 2012

DNA and Cell Biology

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Undetected micrometastasis may play a key role in the early relapse of colorectal cancer (CRC) patients. The aim of this study was to detect circulating tumor cells (CTCs) for predicting early relapse of CRC patients by a weighted enzymatic chip array (WEnCA) and analyze 15 candidate genes associated with CRC carcinogenesis. The genes of 105 postoperative CRC patients were analyzed by membrane array and direct sequencing. We constructed a WEnCA platform including five prognosis-related genes and analyzed the detection rate of WEnCA for CTCs in 30 clinically confirmed CRC relapse patients. Postoperative relapse was significantly correlated with gene overexpression, including EVI2B (p=0.001, OR=4.622), ATP2A2 (p=0.006, OR=4.688), S100B (p=0.001, OR=11.521), TM4SF3 (p=0.001, OR=6.756), and OLFM4 (p=0.008, OR=3.545). Using WEnCA (weighting score of each gene: 5 to EVI2B, 5 to ATP2A2, 12 to S100B, 7 to TM4SF3, and 4 to OLFM4), we could detect CTCs presenting these genotypes in relapsed CRC patients. The sensitivity, specificity, and accuracy were 94.7%, 93.5%, and 97%, respectively. The results of the present study suggest that EVI2B, ATP2A2, S100B, TM4SF3, and OLFM4 could be potential prognostic markers for CRC patients.

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NF1 gene loss of heterozygosity and expression analysis in sporadic colon cancer

Cited by 41 publications

References 25 publications

A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-κB Activation in Colorectal Adenocarcinoma

Colonic Vascular Anomalies and Colon Cancer in Neurofibromatosis: Report of a Case

EVI2B,ATP2A2,S100B,TM4SF3, andOLFM4As Potential Prognostic Markers for Postoperative Taiwanese Colorectal Cancer Patients

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