NFκB- and AP-1-mediated DNA looping regulates matrix metalloproteinase-9 transcription in TNF-α-treated human leukemia U937 cells

Gallic acid-capped gold nanoparticles inhibit EGF-induced MMP-9 expression through suppression of p300 stabilization and NFκB/c-Jun activation in breast cancer MDA-MB-231 cells

“…7). Some studies have shown that MMP-9 expression reflects an interplay between NFκB/AP-1 transcriptional factors and p300-associated acetyltransferase activity (Ma et al, 2004;Mittelstadt and Patel , 2012;Chen and Chang, 2015). Recent studies showed that EGF induces a chromosomal loop formation in the MMP-9 promoter via NFκB/p65…”

“…3E). Previous studies have suggested that p300 and HDAC1 act as co-activator and co-repressor, respectively, for NFB/AP-1-mediated MMP-9 expression (Ma et al, 2004;Mittelstadt and Patel, 2012;Chen and Chang, 2015). Thus, the effect of EGF on p300 and HDAC1 expression was analyzed.…”

“…Real-time RT-PCR was performed in essentially the same manner described previously (Chen and Chang, 2015). Primer sequences used are listed in following:…”

“…Western blot analyses and measurement of cell viability using MTT assay were performed in essentially the same manner as previously described (Chen and Chang, 2015).…”

Exogenous l-arginine reduces matrix metalloproteinase-2 and -9 activities and oxidative stress in patients with hypertension

“…Other possible mechanism is that L-arginine interferes with the transcription of genes related to oxidative stress and inflammatory responses through an NO-independent pathway. It is believed that L-arginine reduces transcription of nuclear factor-κB (NF-κB) and activator protein 1 (AP-1), which are important regulators of MMPs activity [52,55].…”