NGF and BDNF expression drop off in neurally differentiated bone marrow stromal stem cells

Yaghoobi, Mohammad Mehdi; Mahani, Masoud Torkzadeh

doi:10.1016/j.brainres.2008.01.086

Cited by 16 publications

(14 citation statements)

References 22 publications

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“…The mechanism for transdifferentiation of MSCs is unclear, but may result from the induction of neurotrophic factors (NTFs) [21,22]. NTFs are involved in neuroblast proliferation, neural development, and maturation [23,24].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells

Qian

Zhang

et al. 2009

Neurochem Res

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The aim of this study was to compare the neural differentiation potential and the expression of neurotrophic factors (NTFs) in differentiated adipose-derived stem cells (ADSCs) using three established induction protocols, serum free (Protocol 1), chemical reagents (Protocol 2), and spontaneous (Protocol 3) protocols. Protocol 1 produced the highest percentage of mature neural-like cells (MAP2ab(+)). Protocol 2 showed the highest percentage of immature neural-like cells (beta-tubulin III(+)), but the neural-like state was transient and reversible. Protocol 3 caused ADSCs to differentiate spontaneously into immature neural-like cells, but not into mature neural cell types. The neural-like cells produced by Protocol 1 lived the longest in culture with little cell death, but Protocol 2 and 3 led to the significant cell death. Therefore, Protocol 1 is the most efficient among these protocols. Additionally, soon after differentiation, the mRNA levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in dADSCs were sharply decreased by Protocol 1 and 2 (acute induction protocol), but not by Protocol 3 (chronic induction protocol). The results indicate that NTFs played an important role in neural differentiation via acute responses to NGF and BDNF, but not chronically during the transdifferentiation process.

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Section: Introductionmentioning

confidence: 99%

Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells

Qian

Zhang

et al. 2009

Neurochem Res

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“…For instance, MSCs can be easily harvested for autologous transplantation and do not involve ethical issues (Grayson et al, 2015). Although both undifferentiated and differentiated MSCs have been used for cell transplantation in neurological diseases, in vitro differentiation prior to transplantation may be safer than in vivo implantation (Yaghoobi et al, 2008). Thus, determining the ideal conditions to obtain neural cells is necessary.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of neural differentiation of bone marrow mesenchymal stem cells by different induction methods

Zhao

2015

Genet. Mol. Res.

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ABSTRACT. Neurogenic differentiation of bone marrow (BM) mesenchymal stem cells (MSCs) offers a new hope for patients with many neurological disorders. Several chemical inducers are employed to induce BMMSCs differentiation into nerve cells. In the present study, we compared different inducers [2-mercaptoethanol (BME), tretinoin (ATRA), dimethyl sulfoxide/ butylated hydroxyanisole (DMSO/BHA), and indomethacin/3-isobutyl-1-methylxanthine (indomethacin/IBMX)] on the neurogenic differentiation of BMMSCs and aimed to identify a more efficient and safer method. The MSCs were first identified by their ability to adhere to plastic and by the expression of positive (CD44, CD90, and CD105) and negative (CD34) markers assessed by flow cytometry. The efficiency of the neurogenic differentiation was determined by assessing the mRNA and protein expression of nestin, microtubule-associated protein-2 (MAP2), neuron specific enolase (NSE), and glial fibrillary acidic protein (GFAP) by reverse transcription-polymerase chain reaction and western-blot, respectively. The effect of these inducers on cell viability was also evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. This comprehensive study shows that indomethacin/IBMX is better than BME, DMSO/BHA, and ATRA both in terms of efficiency and safety, while BME suppressed the growth and proliferation of MSCs.

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“…Another study suggested that survivin expression levels are cell cycle-dependent and may inhibit cell apoptosis (16). Thus, further studies are required to investigate the survivin response to a variety of different frequencies of ultrasound.…”

Section: Discussionmentioning

confidence: 99%

“…Survivin has been implicated in multiple essential functions, including cell division, programmed cell death or apoptosis, the cellular stress response and checkpoint mechanisms of genomic integrity (15). One study found ~83% BMSCs expressed survivin, with more marked staining in the nucleus than in the cytoplasm (16).…”

Section: Introductionmentioning

confidence: 99%

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Effects of infrasound on the growth of bone marrow mesenchymal stem cells: A pilot study

Fan

2014

Molecular Medicine Reports

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Abstract. Poor viability of transplanted bone marrow mesenchymal stem cells (BMSCs) is well-known, but developing methods for enhancing the viability of BMSCs requires further investigation. The aim of the present study was to elucidate the effects of infrasound on the proliferation and apoptosis of BMSCs, and to determine the association between survivin expression levels and infrasound on BMSCs. Primary BMSCs were derived from Sprague Dawley rats. The BMSCs, used at passage three, were divided into groups that received infrasound for 10, 30, 60, 90 or 120 min, and control groups, which were exposed to the air for the same durations. Infrasound was found to promote proliferation and inhibit apoptosis in BMSCs. The results indicated that 60 min was the most suitable duration for applied infrasound treatment to BMSCs. The protein and mRNA expression levels of survivin in BMSCs from the two treatment groups that received 60 min infrasound or air, were examined by immunofluorescence and quantitative polymerase chain reaction. Significant differences in survivin expression levels were identified between the two groups, as infrasound enhanced the expression levels of survivin. In conclusion, infrasound promoted proliferation and inhibited apoptosis in BMSCs, and one mechanisms responsible for the protective effects may be the increased expression levels of survivin.

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NGF and BDNF expression drop off in neurally differentiated bone marrow stromal stem cells

Cited by 16 publications

References 22 publications

Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells

Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells

Comparative study of neural differentiation of bone marrow mesenchymal stem cells by different induction methods

Effects of infrasound on the growth of bone marrow mesenchymal stem cells: A pilot study

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