Ni(II) interaction with a peptide model of the human TLR4 ectodomain

Peana, Massimiliano; Zdyb, Karolina; Medici, Serenella; Pelucelli, Alessio; Simula, Giancarlo; Gumienna‐Kontecka, Elżbieta; Zoroddu, Maria Antonietta

doi:10.1016/j.jtemb.2017.07.006

Cited by 20 publications

(16 citation statements)

References 39 publications

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“…These results indicate that nickel-induced Th17 cell development is dependent on the production of IL-23 by human monocyte-derived dendritic cells via toll-like receptor 4 (TLR4), p38 MAPK, NF-jB and Jak-signal transducer and activator of transcription pathways. Similar observations on TLR4 and NF-jB involvement were made by Zoroddu et al (2014), Lin et al (2016), Oblak et al (2015) and Peana et al (2017). Almogren et al (2013) observed a predominance of a Th1 phenotype, based on cytokine expression including IL-4 and IL-10, of lymphocytes collected from patients suffering from nickel allergy, in line with commonly accepted mechanisms of contact sensitivity.…”

Section: Immunotoxic Activity Of Nickelsupporting

confidence: 82%

Update of the risk assessment of nickel in food and drinking water

Schrenk¹,

Bignami²,

Bodin³

et al. 2020

EFS2

101

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The European Commission asked EFSA to update its previous Opinion on nickel in food and drinking water, taking into account new occurrence data, the updated benchmark dose (BMD) Guidance and newly available scientific information. More than 47,000 analytical results on the occurrence of nickel were used for calculating chronic and acute dietary exposure. An increased incidence of postimplantation loss in rats was identified as the critical effect for the risk characterisation of chronic oral exposure and a BMDL 10 of 1.3 mg Ni/kg body weight (bw) per day was selected as the reference point for the establishment of a tolerable daily intake (TDI) of 13 lg/kg bw. Eczematous flare-up reactions in the skin elicited in nickel-sensitised humans, a condition known as systemic contact dermatitis, was identified as the critical effect for the risk characterisation of acute oral exposure. A BMDL could not be derived, and therefore, the lowest-observed-adverse-effect-level of 4.3 lg Ni/kg bw was selected as the reference point. The margin of exposure (MOE) approach was applied and an MOE of 30 or higher was considered as being indicative of a low health concern. The mean lower bound (LB)/upper bound (UB) chronic dietary exposure was below or at the level of the TDI. The 95th percentile LB/UB chronic dietary exposure was below the TDI in adolescents and in all adult age groups, but generally exceeded the TDI in toddlers and in other children, as well as in infants in some surveys. This may raise a health concern in these young age groups. The MOE values for the mean UB acute dietary exposure and for the 95th percentile UB raises a health concern for nickel-sensitised individuals. The MOE values for an acute scenario regarding consumption of a glass of water on an empty stomach do not raise a health concern.

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Section: Immunotoxic Activity Of Nickelsupporting

confidence: 82%

Update of the risk assessment of nickel in food and drinking water

Schrenk¹,

Bignami²,

Bodin³

et al. 2020

EFS2

101

View full text Add to dashboard Cite

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“…The dendritic cells present the ions to immunocompetent T cells causing their proliferation and subsequently inflammation (with the participation of Th CD8+ cells and produced cytokines). A maculopapular rash is usually the first skin symptom [7][8][9][10][11].…”

Section: Mechanismmentioning

confidence: 99%

Metal allergy after the Nuss procedure for pectus excavatum: a review

Gałązka¹,

Leis²,

Kroczek³

et al. 2020

pdia

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Minimally invasive repair of pectus excavatum (MIRPE) technique (the Nuss procedure) is a minimally-invasive method that is commonly used in the treatment of pectus excavatum. An allergic reaction to the metal alloy bar that is implanted in the thorax during the procedure is a reported complication. We briefly review current literature concerning epidemiology, mechanisms and research results of allergic reactions after Nuss bar implantation. This allergic reaction occurs in approximately 2.7% of patients and is caused by metals used in the medical implant. The most common symptoms include fever and skin lesions such as allergic dermatitis. Elevated levels of C-reactive protein is a frequent finding in laboratory tests. In order to minimize the risk of such complications, taking a detailed allergy-based medical history and conducting allergy tests, i.e. patch test are required. Allergic reactions can be managed with conservative treatment such as general or topical glucocorticosteroid therapy and antihistamine agents. Severe allergic reactions can be addressed by implant revision, replacement of the steel bar with a titanium substitute or removal of the stabilization at all. Although the risk of an allergic reaction to titanium is smaller it still exists, the titanium substitute is not routinely used due to its higher cost and lesser plasticity which has a negative impact on matching a stabilizing bar during the surgery. Surgeons treating pectus excavatum should remember about the possible allergic reactions after implantation of the metal bar and be familiar with methods of diagnosis and treatment of those complications.

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“…The deprotonation of the ε-amino group of Lys (not present in Bis-DEH) occurs, in fact, at higher pH, in agreement with what has been reported in the literature for peptides containing this amino acid. 32 , 39 , 40 …”

Section: Results and Discussionmentioning

confidence: 99%

Exploring the Specificity of Rationally Designed Peptides Reconstituted from the Cell-Free Extract of Deinococcus radiodurans toward Mn(II) and Cu(II)

et al. 2020

Self Cite

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A series of five rationally designed decapeptides [DEHGTAVMLK (DP1), THMVLAKGED (DP2), GTAVMLKDEH (Term-DEH), TMVLDEHAKG (Mid-DEH), and DEHGGGGDEH (Bis-DEH)] have been studied for their interactions with Cu(II) and Mn(II) ions. The peptides, constructed including the most prevalent amino acid content found in the cell-free extract of Deinococcus radiodurans (DR), play a fundamental role in the antioxidant mechanism related to its exceptional radioresistance. Mn(II) ions, in complex with these peptides, are found to be an essential ingredient for the DR protection kit. In this work, a detailed characterization of Cu(II) systems was included, because Cu(II)–peptide complexes have also shown remarkable antioxidant properties. All peptides studied contain in their sequence coordinating residues that can bind effectively Mn(II) or Cu(II) ions with high affinity, such as Asp, Glu, and His. Using potentiometric techniques, NMR, EPR, UV–vis, and CD spectroscopies, ESI-MS spectrometry, and molecular model calculations, we explored the binding properties and coordination modes of all peptides toward the two metal ions, were able to make a metal affinity comparison for each metal system, and built a structural molecular model for the most stable Cu(II) and Mn(II) complexes in agreement with experimental evidence.

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Ni(II) interaction with a peptide model of the human TLR4 ectodomain

Cited by 20 publications

References 39 publications

Update of the risk assessment of nickel in food and drinking water

Update of the risk assessment of nickel in food and drinking water

Metal allergy after the Nuss procedure for pectus excavatum: a review

Exploring the Specificity of Rationally Designed Peptides Reconstituted from the Cell-Free Extract of Deinococcus radiodurans toward Mn(II) and Cu(II)

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