Ni–nitrilotriacetic acid-modified quantum dots as a site-specific labeling agent of histidine-tagged proteins in live cells

Kim, Jun Won; Park, Hye-Young; Kim, Jaeseung; Ryu, Jiyoung; Kwon, Do Yoon; Grailhe, Régis; Song, Rita

doi:10.1039/b719434j

Cited by 56 publications

(33 citation statements)

References 24 publications

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“…In the following, larger Au nanoparticles (Brinas et al 2008) and quantum dots (Gupta et al 2008;Kim et al 2008b) have also been modified with NTA-containing ligands, as well as silanized iron oxide nanoparticles (Li et al 2007) by conjugation of amine-containing NTA to carboxylic groups on the particle surface. After loading the NTA groups with bivalent cations, the modified particles could be used for conjugation to proteins with polyhistidine residues.…”

Section: (C) Biomoleculesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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Section: (C) Biomoleculesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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“…Although the NTA groups are first chemically immobilized onto the NPs surface ligands, the binding to peptide is indirect or secondary in this context. The direct benefit of this chemistry is that potentially any peptide containing a His n sequence can be conjugated to any NP functionalized with an NTA group and examples of NP materials thus functionalized to date include Au-NPs [40]; FePt magnetic NPs [37], semiconductor QDs [41], Ni-NPs [42] and Si-NPs [43].…”

Section: Secondary Interactionsmentioning

confidence: 99%

Peptides for Specific Intracellular Delivery and Targeting of Nanoparticles: Implications for Developing nanoparticle-mediated Drug Delivery

Delehanty¹,

Boeneman

Bradburne

et al. 2010

Therapeutic Delivery

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The use of peptides to mediate the delivery and uptake of nanoparticle (NP) materials by mammalian cells has grown significantly over the past 10 years. This area of research has important implications for the development of new therapeutic materials and for the emerging field of NP-mediated drug delivery. In this review, we highlight recent advances in the delivery of various NPs by some of the more commonly employed cellular delivery peptides and discuss important related factors such as NP-peptide bioconjugation, uptake efficiency, intracellular fate and toxicity. We also highlight various demonstrations of therapeutic applications of NP-peptide conjugates where appropriate. The paper concludes with a brief forward-looking perspective discussing what can be expected as this field develops in the coming years.

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“…Because histidine appears in proteins with a relatively lower frequency than, for example, lysine, site-specific fluorescent labeling of polypeptides can be achieved by engineering polyhistidine tag sequences, often of six residues in length, into target polypeptides, as demonstrated in several studies. [13,14] In a recent work, Ma et al characterized a cyclometalated iridiumA C H T U N G T R E N N U N G (III) solvento complex as a selective luminogenic probe for histidine-rich proteins and applied it as a dye to the proteins in sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE). [15] Herein, we further explore the advantage of this histidine-specific iridiumA…”

mentioning

confidence: 99%

Luminescent Peptide Labeling Based on a Histidine‐Binding Iridium(III) Complex for Cell Penetration and Intracellular Targeting Studies

Wang

Jia

Huang

et al. 2011

Chemistry A European J

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Ni–nitrilotriacetic acid-modified quantum dots as a site-specific labeling agent of histidine-tagged proteins in live cells

Abstract: Ni-nitrilotriacetic acid (NTA) functionalized CdSe/ZnS quantum dots (QDs) were exploited as a site-specific labeling agent of histidine-tagged biomolecules in live cells; the QDs were found to be water-soluble, aggregation free and stable for several months.

Cited by 56 publications

References 24 publications

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Peptides for Specific Intracellular Delivery and Targeting of Nanoparticles: Implications for Developing nanoparticle-mediated Drug Delivery

Luminescent Peptide Labeling Based on a Histidine‐Binding Iridium(III) Complex for Cell Penetration and Intracellular Targeting Studies

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