Niche differentiation modulates metabolites abundance and composition in silicon fertilizer amended soil during sugarcane growth

Abstract

Background As one of the vital crops globally, sugarcane (Saccharum officinarum L.) has been one of model crops for conducting metabolome research. Although many studies have focused on understanding bioactive components in specific sugarcane tissues, crucial questions have been left unanswered about the response of metabolites to niche differentiation such as different sugarcane tissues (leaf, stem and root), and soil regions (rhizosphere and bulk) under silicon (Si) amended soils. Here, nontargeted metabolite profiling method was leveraged to assess the similarities and differences in the abundance and community composition of metabolites in the different sugarcane and soil compartments. Identify the compartment-specific expression patterns of metabolites, and their association with cane agronomic traits and edaphic factors. We also investigated the response of sugarcane agronomic traits and edaphic factors to Si amended soil. Results We found that Si fertilizer exhibited the advantages of overwhelmingly promoting the height and theoretical production of cane, and profoundly increased soil Si content by 24.8 and 27.0%, while soil available potassium (AK) was enhanced by 3.07 and 2.67 folds in the bulk and rhizosphere soils, respectively. It was also noticed that available phosphorus (AP) in the rhizosphere soil tremendously increased by 105.5%. We detected 339 metabolites in 30 samples using LC–MS/MS analyses, 161 of which were classified and annotated, including organooxygen compounds (19.9%), carboxylic acids and derivatives (15.5%), fatty acyls (15.5%), flavonoids (4.4%), phenols (4.4%), and benzene and substituted derivatives (3.7%). In addition, the total percentages covered by these core metabolites in each compartment ranged from 94.0% (bulk soil) to 93.4% (rhizosphere soil), followed by 87.4% (leaf), 81.0% (root) and 80.5% (stem), suggesting that these bioactive compounds may have migrated from the belowground tissues and gradually filtered in various aboveground niches of the plant. We also observed that the variations and enrichment of metabolites abundance and community were compartment-specific. Furthermore, some key bioactive compounds were markedly associated with plant growth parameters and soil edaphic. Conclusion Taken together, we hypothesized that Si utilization can exhibit the advantage of enhancing edaphic factors and cane agronomic traits, and variations in metabolites community are tissue-specific.

Metabolomics analysis of the effect of acidification on rhizosphere soil microecosystem of tea tree

“…Secondly, carboxylic acids, hydroxy acids, keto organic sulfonic acids and their derivatives existed in large quantities in soil, which could aggravate soil acidification, destroy the antioxidant defense system of plants, and inhibit plant growth (Liu et al, 2022;Zhao et al, 2022). In contrast, benzene and substituted derivatives, organonitrogen compounds and organooxygen compounds were organic compounds that were difficult to be absorbed by plants in the soils, and the degradation of these substances was conducive to the improvement of C, N and O cycles in the soil (Fallah et al, 2022;Jiang et al, 2022). It could be seen that the content of carboxylic acids, hydroxy acids, keto acids, organic sulfonic acids and their derivatives in tea rhizosphere soil decreased with the increase of soil pH, which effectively maintained the normal operation of antioxidant defense system of tea tree; secondly, the content of aryl halides, benzothiazoles, lactams, azolidines, pyridines and derivatives, steroids and steroid derivatives decreased in soil, which guaranteed the microecological balance of rhizosphere soil and facilitated the propagation of soil microorganisms.…”

Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization

“…Fertilization is generally carried out to improve crop productivity 9 , 10 . For instance, sugarcane growers in Guangxi province apply nitrogen (N) fertilizer at the rate of 600–800 kg N ha −1 , which is 6–8 times more than the average N application rate in Brazil 3 .…”

Plant growth and stress-regulating metabolite response to biochar utilization boost crop traits and soil health

“…The extraction was performed at night at 4°C using 0.8 mL aqueous methanol (methanol: H 2 O 2 , 70:30, v/v) and pure methanol and subsequently centrifuged for 10 min at 10,000 g. We collected, homogenized, and filtered the supernatants (SCAA-104, 0.22 mm pore size; ANPEL Shanghai, China, www.anpel.com.cn/ ). Detailed information regarding further processing and annotation of the samples was documented in recent studies ( Fallah et al., 2022 ).…”