Niche-proxies of Hydrocarbon-impacted Rhizosphere Soil of Weeds of Bodo in Gokana, Rivers State, Nigeria

Abstract

Niche-ecology and isolation studies of microbes from the environment have been described as the bedrock and driving-force for bioprocess industry. This investigation was designed to determine the microbiological quality of weeds growing on aged-crude oil polluted soil. Ten (10) weeds Cyperus esculentus, Scleria pauciflora. Asystasia gangetica Harungana madagascariensis, Ancistoclaudus tectorius, Kyllinga erecta, Cinna arundinacea, Brassica chinensis, Cyperus difformis, Kyllinga bulbosa and Brachiaria mutica and their rhizospheric soil were obtained from Bodo, Gokana LGA, Sludge farm and Botanical garden of the University of Port Harcourt, Rivers State, Nigeria. The soil was enriched in Mineral Salt Media and Bonny Light Crude Oil, prior to the spread-plating on solidified media. Result of the analysis showed pH of soil samples ranged from 5.26-7.2; Electrical conductivity was 53.4-80.31µS/cm, and phosphate 0.74-5.35 mg/kg. Levels of Vanadium in pre-impacted rhizosoil obtained from Kyllinga erecta and Cinna arundinaceae were 0.61 and 0.70 mg/kg respectively. Moisture content of soil obtained from polluted and pristine environments were 11.75% and 17.82% respectively. Permeability indices were 9.0 describing the pristine soil to have low plasticity. Total heterotrophic bacterial count was within 3.5-8.0 Log10 Cfu/g distributed among the weed rhizospheres. Cyperus esculentus rhizosphere soil was more dominated with others like Achromobacter sp, B. lichenformis, B. anthracis, B. subtilis, B. fumari, Arthrobacter sp, Pseudomonas sp, P. aeruginosa, P. fluorescens. Fungal isolates were Aspergillus terreus, Trichoderma sp, and Fusarium sp. These findings further support the rhizosphere of plants as a rich bioresource for biomining of high throughput strains for biotechnological application.

Parameter Affinity Estimation of Rhizobacterial Cocktail Formulations for Hydrocarbon Degradation Using Locally Available Substrates in Crude Oil-Impacted Soil

“…Uquetan et al [36] have identified the influence of crude oil and hydrocarbons on crop productivity and yield. They emphasize that hydrocarbons within crude oil-contaminated soil disrupt the soil's physical, chemical [37], and microbiological [13,[38][39][40] properties. These disruptions significantly contribute to diminished crop productivity, particularly impacting the functional roles of soil organisms.…”

“…A wide array of organic waste holds potential for bioenergy production and serves as a medium amendment for cultivating valuable resources. The ascendancy of agrowaste as feedstocks and substrates for microbial product synthesis underscores their capacity to provide essential nutrients [40,47]. Biotechnological applications leverage agro-waste for nutrient supply in biostimulation processes, as immobilization matrices for starter cultures or inocula, and as supplements for lipid biosynthesis [48].…”

“…The ascendancy of agrowaste as feedstocks and substrates for microbial product synthesis underscores their capacity to provide essential nutrients [40,47]. Biotechnological applications leverage agro-waste for nutrient supply in biostimulation processes, as immobilization matrices for starter cultures or inocula, and as supplements for lipid biosynthesis [48]. Notable materials, including banana peels, yam peels, potato peels, cassava peels, rice husks, sugarcane bagasse, and oil palm residues, serve as sources of carbon whilst concurrently acting as conditioners and absorbents (Table 1) [56,57].…”

Antibiotics Susceptibility Pattern of Bacterial Isolates Obtained from Potable Water Sources in Okerenkoko Community, Delta State, Nigeria

“…Ten-fold serial (10-fold) using sterile distilled water as a diluent. Cultured plates were counted for visible colonies and presented in CFU/ml (Effiong et al, 2020).…”