Nickel Affects Activity More Than Expression of Hydrogenase Protein in Frankia

Mattsson, Ulrika; Sellstedt, Anita

doi:10.1007/s00284-001-0056-y

Cited by 14 publications

(6 citation statements)

References 22 publications

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“…Specifics on the uptake hydrogenase of the Parasponia-rhizobia symbiosis are not known, but the system is known to be active due to the negligible levels of H 2 evolution from nodules as a proportion of total nitrogenase activity (Becking, 1983b). Likewise, uptake hydrogenase activity is widespread in Frankia (Benson et al, 1980;Mattsson and Sellstedt, 2002;Roelofsen and Akkermann, 1979). Antisera raised against the large subunit of Bradyrhizobium japonicum hydrogenase recognized this protein in Frankia KB5 (Mattsson et al, 2001).…”

Section: Evolutionary and Ecological Considerationsmentioning

confidence: 96%

Root-based N2-fixing Symbioses: Legumes, Actinorhizal Plants, Parasponia sp. and Cycads

2005

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In the mutualistic symbioses between legumes and rhizobia, actinorhizal plants and Frankia, Parasponia sp. and rhizobia, and cycads and cyanobacteria, the N 2 -fixing microsymbionts exist in specialized structures (nodules or cyanobacterial zones) within the roots of their host plants. Despite the phylogenetic diversity among both the hosts and the microsymbionts of these symbioses, certain developmental and physiological imperatives must be met for successful mutualisms. In this review, phylogenetic and ecological aspects of the four symbioses are first addressed, and then the symbioses are contrasted and compared in regard to infection and symbio-organ development, supply of carbon to the microsymbionts, regulation of O 2 flux to the microsymbionts, and transfer of fixed-N to the hosts. Although similarities exist in the genetics, development, and functioning of the symbioses, it is evident that there is great diversity in many aspects of these root-based N 2 -fixing symbioses. Each symbiosis can be admired for the elegant means by which the host plant and microsymbiont integrate to form the mutualistic relationships so important to the functioning of the biosphere.

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Section: Evolutionary and Ecological Considerationsmentioning

confidence: 96%

Root-based N2-fixing Symbioses: Legumes, Actinorhizal Plants, Parasponia sp. and Cycads

2005

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“…Previous reports described the effects of nickel on the hydrogenase expression and activity (5,18,31,37,45). Our analysis at the functional, protein, and mRNA levels of HynSL in AltDE cells further reveals the mechanisms that govern the effects of nickel.…”

Section: Discussionmentioning

confidence: 99%

[NiFe] Hydrogenase from Alteromonas macleodii with Unusual Stability in the Presence of Oxygen and High Temperature

Vargas

Weyman

Ying-kai

et al. 2011

Appl Environ Microbiol

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Hydrogenases are enzymes involved in the bioproduction of hydrogen, a clean alternative energy source whose combustion generates water as the only end product. In this article we identified and characterized a [NiFe] hydrogenase from the marine bacterium Alteromonas macleodii "deep ecotype" with unusual stability toward oxygen and high temperature. The A. macleodii hydrogenase (HynSL) can catalyze both H 2 evolution and H 2 uptake reactions. HynSL was expressed in A. macleodii under aerobic conditions and reached the maximum activity when the cells entered the late exponential phase. The higher level of hydrogenase activity was accompanied by a greater abundance of the HynSL protein in the late-log or stationary phase. The addition of nickel to the growth medium significantly enhanced the hydrogenase activity. Ni treatment affected the level of the protein, but not the mRNA, indicating that the effect of Ni was exerted at the posttranscriptional level. Hydrogenase activity was distributed ϳ30% in the membrane fraction and ϳ70% in the cytoplasmic fraction. Thus, HynSL appears to be loosely membrane-bound. Partially purified A. macleodii hydrogenase demonstrated extraordinary stability. It retained 84% of its activity after exposure to 80°C for 2 h. After exposure to air for 45 days at 4°C, it retained nearly 100% of its activity when assayed under anaerobic conditions. Its catalytic activity in the presence of O 2 was evaluated by the hydrogen-deuterium (H-D) exchange assay. In 1% O 2 , 20.4% of its H-D exchange activity was retained. The great stability of HynSL makes it a potential candidate for biotechnological applications.

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“…Hydrogenases oxidize molecular dihydrogen, which is also a by‐product of nitrogenase. These enzymes channel electrons into electron transport phosphorylation to make ATP and thus help the nitrogen‐fixing cells to be more efficient when they perform the very energy‐demanding reduction to ammonia (Mattsson and Sellstedt 2002). Many nitrogen‐fixing bacteria maintain hydrogenases termed uptake hydrogenases ( hup ) to recover the loss of energy and reductant increasing efficiency.…”

Section: Genome Projectsmentioning

confidence: 99%

Exploring the genomes of Frankia

Normand

Queiroux

Tisa

et al. 2007

Physiologia Plantarum

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The recent determination of the genome sequence of three Frankia strains has highlighted the evolutionary forces that have shaped the genetic makeup of the actinorhizal symbionts and it has opened up many avenues of research. Instances of gene duplication, gene loss and gene acquisition through lateral transfer show that the three Frankia genomes are dynamic and have evolved as a function of their host characteristics and biogeography. No convincing nod gene cluster or significant symbiotic island could be discerned. All the genes presently known to be involved in the symbiosis (nif, hup1 and hup2, shc) are found spread over the genome in at least four clusters. The results will be discussed with emphasis on understanding the mechanisms underlying the interaction and link between evolutionary forces and ecological adaptation to different biotopes.

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Nickel Affects Activity More Than Expression of Hydrogenase Protein in Frankia

Cited by 14 publications

References 22 publications

Root-based N2-fixing Symbioses: Legumes, Actinorhizal Plants, Parasponia sp. and Cycads

Root-based N2-fixing Symbioses: Legumes, Actinorhizal Plants, Parasponia sp. and Cycads

[NiFe] Hydrogenase from Alteromonas macleodii with Unusual Stability in the Presence of Oxygen and High Temperature

Exploring the genomes of Frankia

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