Nickelocene‐mediated glucose oxidase electrode

Abstract

Nickelocene is investigated as an electrochemical mediator in glucose oxidase (GOD) amperometric electrodes. It is found that the adsorbed nickelocene mediates the charge transfer between the electrode and glucose oxidase adsorbed on the electrode surface. The quasi redox potential of a nickelocene/nickelocenium couple adsorbed on an electrochemically activated pyrolytic graphite electrode is experimentally estimated ( -0.105 V vs. Ag/AgCl). The steady-state amperometric response of nickelocene-mediated glucose electrode is investigated as a function of the glucose concentration, applied potential, and solution pH of the electrolyte. The working potential of a nickelocene-mediated glucose electrode is 0.00 V (vs. Ag/AgCl).

Enzyme-Based Biosensors for in Vivo Measurements

“…In addition to ferrocene derivatives including those attached to polymers, other mediators have also been investigated. Nickelocene has a lower formal potential than ferrocene but also reacts more slowly with the enzyme . Phenazines, phenoxazines, and Wurster's salts have been evaluated as possible mediators .…”

Glucose oxidase entrapped in polypyrrole on high-surface-area Pt electrodes: a model platform for sensitive electroenzymatic biosensors

“…However, a major challenge in the design of these sensors is the diminished sensitivity that comes with the electrode miniaturization required of many applications. A number of strategies have been pursued in attempts to overcome this problem including the incorporation of additional electron transfer mediators [9], use of sol-gels as the enzyme immobilization matrix to increase enzyme 0022-0728/$ -see front matter Ó 2004 Elsevier B.V. All rights reserved. doi:10.1016/j.jelechem.2004.08.023 loading [10], and the employment of porous electrode materials.…”