NiCo2S4 nanoparticle-dispersed MoS2 nanosheets for catalytic and sensitive electrochemical aptamer sensing of ochratoxin A via cascaded amplifications

Gao, Huahui; Yao, Jianglong; Jiang, Bingying; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.snb.2022.132530

Cited by 12 publications

(7 citation statements)

References 34 publications

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“…A detection limit of 0.95 pM was achieved with a linear range for cTnI detection from 10 pM to 1.0 µM, as shown in Figure 11B. In another recent study in 2022 by Gao et al [72], electrochemical aptamer sensing of ochratoxin A (OTA), one of the most toxic mycotoxins in food, via cascaded amplifications was achieved using NiCo 2 S 4 nanoparticle-dispersed MoS 2 nanosheets. The sensor was based on the DNAzyme-powered DNA walker along with hybridization chain reaction-amplification strategies.…”

Section: Molybdenum Disulfide Nanosheet-based Sensorsmentioning

confidence: 90%

Aptamer-Based Optical and Electrochemical Sensors: A Review

Farid,

Ghosh,

Dutta

et al. 2023

Chemosensors

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There is a pressing need to identify recent directions in the field of aptamer-based sensing. DNA aptamers that are synthetically generated by in vitro selection mechanisms using the SELEX technique are single-stranded oligonucleotides which are selected to bind to a target with favorable sensitivity and selectivity. These aptamers have attracted significant attention due to their high binding affinity and ability to be easily engineered and provide various detection modes in what are known as aptasensors. Our aim is to focus on specialized detection strategies that have gained less attention but are of vital importance, such as optical detection in live cells, fluorescence polarization sensing, multi-analyte detection, colorimetric bioassays, wavelength shifting, and electrochemical-based detection. This will provide us with a perspective to facilitate developments in aptasensor technology for various targets, promising a bright future for biological receptors in the field of biosensing.

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Section: Molybdenum Disulfide Nanosheet-based Sensorsmentioning

confidence: 90%

Aptamer-Based Optical and Electrochemical Sensors: A Review

Farid,

Ghosh,

Dutta

et al. 2023

Chemosensors

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“…Aptamers are functionalized RNA or ssDNA that bind specifically to specific targets and are screened by systematic evolution of ligands by the exponential enrichment technique (SELEX), which are known as artificial antibodies. − Due to the advantages of high specificity, easy preparation, high affinity, low immunogenicity, and a wide target range, aptamers are increasingly used in food, medicine, analytical chemistry, and other fields. ,, Particularly, aptamers have been recently reported as new molecular recognition elements for the detection of sialic acid or sialic acid modified sugars. , Therefore, it is promising to develop new aptamer-based biosensors for 6′-SL detection. So far, diverse technologies including electrochemistry, colorimetry, fluorescence, , electrochemiluminescence, and photoelectrochemistry have been applied for the construction of aptamer-based biosensors. However, few of them can be used to visualize and monitor α-2,3-linked or α-2,6-linked Neu5Ac in living cells.…”

Section: Introductionmentioning

confidence: 99%

“…for 6′-SL detection. So far, diverse technologies including electrochemistry,16 colorimetry, 17 fluorescence,10,14 electrochemiluminescence,18 and photoelectrochemistry 19 have been applied for the construction of aptamer-based biosensors. However, few of them can be used to visualize and monitor α-2,3-linked or α-2,6-linked Neu5Ac in living cells.…”

mentioning

confidence: 99%

Screening of a Sialyllactose-Specific Aptamer and Engineering a Pair of Recognition Elements with Unique Fluorescent Characteristics for Sensitive Detection of Sialyllactose

Chen

Zhang

et al. 2023

J. Agric. Food Chem.

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A single-stranded DNA (ssDNA) aptamer specific for 6′-sialyllactose (6′-SL) was screened through magnetic separation-based SELEX and post-SELEX truncation and used to construct unique aptamer bio-dots for sensitive detection of 6′-SL. Eighteen rounds of screening were conducted during the SELEX process. The ssDNA aptamer Apt9 (K d = 152.3 nM) with a length of 79 nucleotides (nt) was demonstrated as the optimal aptamer candidate after affinity and specificity evaluation. Then, Apt9 was truncated and optimized according to secondary structure and molecular docking. A 35 nt truncated aptamer Apt9-1 (K d = 91.75 nM) with higher affinity than Apt9 was finally obtained. Furthermore, Apt9-1 was used to synthesize bio-dots as a new recognition element of 6′-SL, and the aminobenzene boric acid functionalized carbon dots were employed as the other recognition element. With the respective fluorescent characteristics, the two quantum dots (QDs) were made a pair to construct a 6′-SL fluorescent biosensor. The linear detection range of the biosensor is 10 μM to 5 mM, and the detection limit is 0.9 μM. With the advantages of time-saving, high efficiency, and simplicity in the actual sample detection, the screened aptamer and dual-QD-based biosensor have broad application prospects in 6′-SL detection.

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“…31,32 In addition, DNAzymes possess several unique properties, such as high catalytic activity, multiple turnover capability and being free of stringent reaction conditions. [33][34][35][36] These advantages make DNAzymes attractive signal amplification alternatives for the construction of various sensitive biosensors. Here, using a new self-constrained DNAzyme and HCR, we report a dually amplified and label-free fluorescence aptamer sensor to sensitively detect sarafloxacin in diluted milk samples.…”

Section: Introductionmentioning

confidence: 99%