NiCoP/g-C3N4 Nanocomposites-Based Electrochemical Immunosensor for Sensitive Detection of Procalcitonin

Abstract

Herein, an ultra-sensitive and facile electrochemical biosensor for procalcitonin (PCT) detection was developed based on NiCoP/g-C3N4 nanocomposites. Firstly, NiCoP/g-C3N4 nanocomposites were synthesized using hydrothermal methods and then functionalized on the electrode surface by π-π stacking. Afterward, the monoclonal antibody that can specifically capture the PCT was successfully linked onto the surface of the nanocomposites with a 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) condensation reaction. Finally, the modified sensor was employed for the electrochemical analysis of PCT using differential Pulse Voltammetry(DPV). Notably, the larger surface area of g-C3N4 and the higher electron transfer capacity of NiCoP/g-C3N4 endow this sensor with a wider detection range (1 ag/mL to 10 ng/mL) and an ultra-low limit of detection (0.6 ag/mL, S/N = 3). In addition, this strategy was also successfully applied to the detection of PCT in the diluted human serum sample, demonstrating that the developed immunosensors have the potential for application in clinical testing.

Emerging Roles of Graphitic Carbon Nitride-based Materials in Biomedical Applications

“…The ratiometric fluorescence sensor is rapid and sensitive in exosome detection. 105 The sensor is capable of detecting PCT in diluted serum with a wide linear range (1 ag/mL to 10 ng/mL) and very low detection limit (0.6 ag/mL), which is expected to provide a new method for detecting PCT in clinical applications. 108 The anodic ECL emission was obtained on BPNSs modified electrode using tripropylamine (TPrA) as a coreactant, and the cathodic ECL emission was obtained using g-C 3 N 4 and K 2 S 2 O 8 as coreactants, and cholesterol oxidase was immobilized on a glassy carbon electrode, which reduced the false positive signal due to the separation of anodic and cathodic ECL signals of BPNSs and g-C 3 N 4 .…”

“…Procalcitonin is a commonly used clinical marker for bacterial infections, and the concentration of PCT in blood is often used as an important diagnostic criterion to evaluate the severity of bacterial infections. − Chen et al constructed an electrochemical sensor by p-p stacking, synthesizing NiCoP/g-C 3 N 4 composite to functionalize the electrode, and attaching a monoclonal antibody to the surface of the material to capture PCT . The sensor is capable of detecting PCT in diluted serum with a wide linear range (1 ag/mL to 10 ng/mL) and very low detection limit (0.6 ag/mL), which is expected to provide a new method for detecting PCT in clinical applications.…”

Fabrication of Gold-Graphitic Carbon Nitride Composite Electrode for the Detection of Pimobendan

“…The C3N4 showed a flake-like morphology of TEM images in Figure 3 and Figure 4. Graphitic carbon nitride (g-C3N4) is a two-dimensional organic semiconductor with a high specific surface area, additionally g-C3N4 owns high electrical conductivity for its sole delocalized conjugated structure [22][23][24][25][26]. Au is applied in promotion of some oxidation-reduction reactions and electrochemical reaction system [12,16,21].…”