Nicotianamine Chelates Both FeIII and FeII. Implications for Metal Transport in Plants1

Wirén, Nicolaus von; Klair, Sukhbinder S.; Bansal, Suhkibar; Briat, Jean‐François; Khodr, Hicham; Shioiri, Takayuki; Leigh, R. A.; Hider, Robert C.

doi:10.1104/pp.119.3.1107

Cited by 426 publications

(367 citation statements)

References 32 publications

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“…The sensing mechanisms are not known, but the metal chelator nicotianamine is important for Fe homestasis 35,36 and can bind both Fe and Cu. 37 We also showed that under Fe deficiency, plants were strikingly more susceptible to Cu toxicity. Understanding this aspect of Fe-Cu crosstalk could have implications for agriculture and for phytoremediation of metal contaminated soils.…”

mentioning

confidence: 76%

Optimal copper supply is required for normal plant iron deficiency responses

Waters

Armbrust

2013

Plant Signaling & Behavior

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mentioning

confidence: 76%

Optimal copper supply is required for normal plant iron deficiency responses

Waters

Armbrust

2013

Plant Signaling & Behavior

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“…This unusual chlorosis pattern may be the result of a redistribution of the cytoplasmic NA pool to the chloroplast. This has the dual effect of restricting NA from performing its role in phloem transport of iron and other metals (von Wiren et al, 1999) and also sequestering iron itself, thus preventing it from being redistributed throughout the plant body. Iron, applied in excess (300 mM), is able to rescue the chlorosis phenotype, and the MAR1 gene is down-regulated under iron deficiency to prevent sequestration of needed iron.…”

Section: Discussionmentioning

confidence: 99%

Multiple Antibiotic Resistance in Arabidopsis Is Conferred by Mutations in a Chloroplast-Localized Transport Protein

et al. 2009

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Widespread antibiotic resistance is a major public health concern, and plants represent an emerging antibiotic exposure route. Recent studies indicate that crop plants fertilized with antibiotic-laden animal manure accumulate antibiotics; however, the molecular mechanisms of antibiotic entry and subcellular partitioning within plant cells remain unknown. Here, we report that mutations in the Arabidopsis (Arabidopsis thaliana) locus Multiple Antibiotic Resistance1 (MAR1) confer resistance, while MAR1 overexpression causes hypersensitivity to multiple aminoglycoside antibiotics. Additionally, yeast expressing MAR1 are hypersensitive to the aminoglycoside G418. MAR1 encodes a protein with 11 putative transmembrane domains with low similarity to ferroportin1 from Danio rerio. A MAR1:yellow fluorescent protein fusion localizes to the chloroplast, and chloroplasts from plants overexpressing MAR1 accumulate more of the aminoglycoside gentamicin, while mar1-1 mutant chloroplasts accumulate less than the wild type. MAR1 overexpression lines are slightly chlorotic, and chlorosis is rescued by exogenous iron. MAR1 expression is also down-regulated by low iron. These data suggest that MAR1 is a plastid transporter that is likely to be involved in cellular iron homeostasis and allows opportunistic entry of multiple antibiotics into the chloroplast.

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“…NA is also thought to play an important role in the detoxification of excess intracellular Fe (von Wirén et al, 1999). NA concentrations in tomato increase in response to Fe overload (Pich et al, 2001).…”

Section: Two Different Expression Patterns With Different Localizationmentioning

confidence: 99%

Three Nicotianamine Synthase Genes Isolated from Maize Are Differentially Regulated by Iron Nutritional Status

et al. 2003

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Nicotianamine synthase (NAS) is an enzyme that is critical for the biosynthesis of the mugineic acid family of phytosiderophores in graminaceous plants, and for the homeostasis of metal ions in nongraminaceous plants. We isolated one genomic NAS clone, ZmNAS3, and two cDNA NAS clones, ZmNAS1 and ZmNAS2, from maize (Zea mays cv Alice). In agreement with the increased secretion of phytosiderophores with Fe deficiency, ZmNAS1 and ZmNAS2 were positively expressed only in Fe-deficient roots. In contrast, ZmNAS3 was expressed under Fe-sufficient conditions, and was negatively regulated by Fe deficiency. This is the first report describing down-regulation of NAS gene expression in response to Fe deficiency in plants, shedding light on the role of nicotianamine in graminaceous plants, other than as a precursor in phytosiderophore production. ZmNAS1-green fluorescent protein (sGFP) and ZmNAS2-sGFP were localized at spots in the cytoplasm of onion (Allium cepa) epidermal cells, whereas ZmNAS3-sGFP was distributed throughout the cytoplasm of these cells. ZmNAS1 and ZmNAS3 showed NAS activity in vitro, whereas ZmNAS2 showed none. Due to its duplicated structure, ZmNAS2 was much larger (65.8 kD) than ZmNAS1, ZmNAS3, and previously characterized NAS proteins (30-38 kD) from other plant species. We reveal that maize has two types of NAS proteins based on their expression pattern and subcellular localization.To acquire Fe, graminaceous plants secrete Fe chelators, known as mugineic-acid family phytosiderophores (MAs). MAs dissolve Fe in the rhizosphere, followed by reabsorption of the Fe(III)-MA complexes through YS1 transporters in the plasma membrane (Takagi, 1976; Curie et al., 2001). Only graminaceous plants use the MA mechanism of acquiring Fe(III), classified as the Strategy II mechanism (Marschner et al., 1986). Fe deficiency is a problem in crop production worldwide, especially in calcareous soils, where Fe is sparingly soluble due to the high soil pH. The ability of graminaceous plants to tolerate Fe deficiency is thought to depend on the quantity of MAs secreted during Fe deficiency (Takagi, 1976;Mori et al., , 1988Rö mheld, 1987;Kawai et al., 1988;Mihashi and Mori, 1989;Singh et al., 1993). The biosynthetic pathways of MAs (Fig. 1A) have been determined Kawai et al., 1988;Shojima et al., 1990;Ma et al., 1999), and almost all the genes involved have been isolated in our laboratory (Higuchi et al., 1999b;Takahashi et al., 1999;Nakanishi et al., 2000;Kobayashi et al., 2001). NAS is a key enzyme in MA biosynthesis, catalyzing the trimerization of SAM into one molecule of NA (Higuchi et al., 1999b). NAS activity in graminaceous plants is well correlated with tolerance to Fe deficiency. In maize (Zea mays), a plant susceptible to Fe deficiency, NAS activity is very low (Higuchi et al., 1996a), and maize secretes lower amounts of MAs than barley or oat (Avena sativa), cereals that are tolerant of low Fe supply (Rö mheld, 1987;Lytle and Jolley, 1991). NAS is also important for growth in nongraminaceous plants, which do not ...

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Nicotianamine Chelates Both FeIII and FeII. Implications for Metal Transport in Plants1

Cited by 426 publications

References 32 publications

Optimal copper supply is required for normal plant iron deficiency responses

Optimal copper supply is required for normal plant iron deficiency responses

Multiple Antibiotic Resistance in Arabidopsis Is Conferred by Mutations in a Chloroplast-Localized Transport Protein

Three Nicotianamine Synthase Genes Isolated from Maize Are Differentially Regulated by Iron Nutritional Status

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