Nicotinamide inhibition of <i>Xenopus laevis</i> oocyte maturation

Godeau, François; Sahni, Mukesh K.; Boquet, Patrice; Koide, S. S.

doi:10.1002/mrd.1120140207

Cited by 5 publications

(1 citation statement)

References 41 publications

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“…Xenopus oocytes were isolated from the ovaries of mature female animals as described in a previous report {Godeau, Sahni, Bouquet and Koide 1986). Oocytes at stage VI of Dumont (1972) were selected by viewing under a stereomicroscope and cultivated in Modified Barth Saline Hepes (MBSH) medium.…”

Section: Xenopus Oocyte Maturation Inhibiting Assaymentioning

confidence: 99%

Inhibition of Progesterone Action by a Factor in Human Follicular Fluid

Juneja

Kadam²,

Al³

et al. 1993

Horm Metab Res

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A factor was found in human follicular fluid that blocked progesterone-stimulated net uptake of 45Ca2+ in human sperm and progesterone-induced maturation of Xenopus oocyte. The factor was partially purified by ultrafiltration through PM-10 membrane and gel filtration on Sephadex G-25 column. The active fraction is effective at a concentration of 200 micrograms/ml. The present findings suggest that hFF factor may regulate the metabolism of progesterone sensitive cells.

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Section: Xenopus Oocyte Maturation Inhibiting Assaymentioning

confidence: 99%

Inhibition of Progesterone Action by a Factor in Human Follicular Fluid

Juneja

Kadam²,

Al³

et al. 1993

Horm Metab Res

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Inhibition of serotonin‐induced oocyte maturation by a Spisula factor

Kadam

Koide

1990

J. Exp. Zool.

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A factor that blocks serotonin (5-hydroxytryptamine, 5-HT)-induced maturation of Spisula oocytes was purified from Spisula oocytes by ultrafiltration through a PM-10 membrane, charcoal adsorption, and reversed-phase HPLC on a Partisil ODS-3 column. The oocyte factor is a heat-stable, non-proteinaceous compound with an estimated Mr of less than 1,000 Da. It blocked 5-HT-induced maturation of Spisula ooctyes at concentrations ranging from 10 to 100 kg/ml. It did not influence KC1-or potassium-glutamate-induced maturation of Spisula oocytes or the maturation of mouse, Chaetopterus, Xenopus, or Asterias oocytes. The oocyte substance may function as a modulator of 5-HT action andlor be the endogenous meiotic arresting factor in Spisula oocytes. At concentrations exceeding 100 kl/ml, the oocyte factor induced formation of blebs and cytoplasmic droplets.

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Inhibition of progesterone‐mediated maturation of oocytes of Xenopus laevis by oocyte maturation inhibitor from pig follicular fluid: Development of a routine assay for the inhibitor with Xenopus oocytes

Pomerantz

Bilello

1987

Gamete Research

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We describe an assay for oocyte maturation inhibitor (OMI) using the progesterone-mediated maturation of Xenopus oocytes. The test fraction used was a partially purified fraction from pig follicular fluid, which gave consistent inhibition of maturation in the pig oocyte assay. In the toad assay, oocytes (30-50) from each toad were pretested to determine whether satisfactory maturation was achieved because widespread animal variation was observed. Toads whose oocytes showed greater than 60% maturation in the pretest could be used directly. Toads whose oocytes showed less than 60% maturation were injected with pregnant mares serum gonadotropin (PMSG) in order to increase progesterone-mediated maturation. The dose and time after injection of PMSG before harvesting oocytes, dose and duration of progesterone exposure, and order of exposure of oocytes to OMI and progesterone were important variables. Opposing effects of OMI and progesterone were seen in oocytes from toads receiving 60 IU PMSG. In the routine assay we use animals whose oocytes show greater than 60% maturation in the pretest or animals treated with 12 IU of PMSG 4 to 7 days before use. Oocytes are exposed to the OMI fraction for 1 hr, progesterone is added, and incubations continued until controls reach maximum maturation (5 to 8 hr). The inhibition of toad oocyte maturation by OMI is reversible. The toad and mammalian oocyte assays were compared using more highly purified fractions of OMI and gave identical results.

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Nicotinamide inhibition of Xenopus laevis oocyte maturation

Cited by 5 publications

References 41 publications

Inhibition of Progesterone Action by a Factor in Human Follicular Fluid

Inhibition of Progesterone Action by a Factor in Human Follicular Fluid

Inhibition of serotonin‐induced oocyte maturation by a Spisula factor

Inhibition of progesterone‐mediated maturation of oocytes of Xenopus laevis by oocyte maturation inhibitor from pig follicular fluid: Development of a routine assay for the inhibitor with Xenopus oocytes

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