Nicotine levels in silicone wristband samplers worn by children exposed to secondhand smoke and electronic cigarette vapor are highly correlated with child’s urinary cotinine

Quintana, Penelope J E; Hoh, Eunha; Dodder, Nathan G.; Matt, Georg E.; Zakarian, Joy M.; Anderson, Kim A.; Akins, Brittany; Chu, Linda C.; Hovell, Melbourne F.

doi:10.1038/s41370-019-0116-7

Cited by 56 publications

(53 citation statements)

References 32 publications

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“…However, relative to traditional biomarkers, the wristbands have the considerable advantage of enabling the assessment of multitudes of chemical pollutants in a single passive instrument. While previous studies have demonstrated promising relations between specific classes of exposures [e.g., PAHs ( 16 ), nicotine ( 67 ), organophosphate flame retardants ( 21 , 68 )] assessed in wristbands and traditional biomarkers of exposure, to our knowledge, no work has yet assessed relations between the broad screen of chemicals used in our study with traditional biomarkers, and this is an important area for future research.…”

Section: Discussionmentioning

confidence: 93%

Assessment of Multipollutant Exposures During Pregnancy Using Silicone Wristbands

Doherty

Pearce

Anderson

et al. 2020

Front. Public Health

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Section: Discussionmentioning

confidence: 93%

Assessment of Multipollutant Exposures During Pregnancy Using Silicone Wristbands

Doherty

Pearce

Anderson

et al. 2020

Front. Public Health

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“…Urine samples. Single spot urine samples were collected from child participants using procedures from our previous studies 12,14,15 . Caregivers were asked to have the child collect their sample on F o r P e e r R e v i e w the morning of the second home visit, but sometimes samples were collected at other times due to child and caregiver schedules.…”

Section: Daily Monitoringmentioning

confidence: 99%

“…The estimated method detection limit (MDL) 19 was 0.19 ng/wristband. Detailed information on the sample preparation, extraction and quantification of nicotine from wristbands has been described 12 .…”

Section: Laboratory Analysismentioning

confidence: 99%

“…Urinary Cotinine. Urinary cotinine was determined though isotope dilution LC-MS/MS 12,14,15 .The estimated MDL was 0.033 ng/mL urine, and cotinine levels were reported in ng/mL urine 20 .…”

Section: Laboratory Analysismentioning

confidence: 99%

“…Our group was the first to apply silicone wristbands to measurement of tobacco product exposure in children, and we demonstrated that nicotine levels in silicone wristbands worn by children (n = 31) for 2 and 7 days were both highly correlated with cotinine, a metabolite of nicotine, in urine from the same child 12 . Here, we further investigated the ability of silicone wristbands to record differences in nicotine exposures among children recruited into different exposure groups: children exposed to SHS or those exposed to SHeV compared to children living with non-users of either conventional or electronic cigarettes.…”

Section: Introductionmentioning

confidence: 98%

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Nicotine, Cotinine, and Tobacco-Specific Nitrosamines Measured in Children’s Silicone Wristbands in Relation to Secondhand Smoke and E-cigarette Vapor Exposure

Quintana

López-Gálvez

Dodder

et al. 2020

Nicotine &Amp; Tobacco Research

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Introduction Simple silicone wristbands (WB) hold promise for exposure assessment in children. We previously reported strong correlations between nicotine in WB worn by children and urinary cotinine (UC). Here, we investigated differences in WB chemical concentrations among children exposed to secondhand smoke from conventional cigarettes (CC) or secondhand vapor from electronic cigarettes (EC), and children living with nonusers of either product (NS). Methods Children (n = 53) wore three WB and a passive nicotine air sampler for 7 days and one WB for 2 days, and gave a urine sample on day 7. Caregivers reported daily exposures during the 7-day period. We determined nicotine, cotinine, and tobacco–specific nitrosamines (TSNAs) concentrations in WB, nicotine in air samplers, and UC through isotope-dilution liquid chromatography with triple-quadrupole mass spectrometry. Results Nicotine and cotinine levels in WB in children differentiated between groups of children recruited into NS, EC exposed, and CC exposed groups in a similar manner to UC. WB levels were significantly higher in the CC group (WB nicotine median 233.8 ng/g silicone, UC median 3.6 ng/mL, n = 15) than the EC group (WB nicotine median: 28.9 ng/g, UC 0.5 ng/mL, n = 19), and both CC and EC group levels were higher than the NS group (WB nicotine median: 3.7 ng/g, UC 0.1 ng/mL, n = 19). TSNAs, including the known carcinogen NNK, were detected in 39% of WB. Conclusions Silicone WB show promise for sensitive detection of exposure to tobacco-related contaminants from traditional and electronic cigarettes and have potential for tobacco control efforts. Implications Silicone WB worn by children can absorb nicotine, cotinine, and tobacco-specific nitrosamines, and amounts of these compounds are closely related to the child’s urinary cotinine. Levels of tobacco-specific compounds in the silicone WB can distinguish patterns of children’s exposure to secondhand smoke and e-cigarette vapor. Silicone WB are simple to use and acceptable to children and, therefore, may be useful for tobacco control activities such as parental awareness and behavior change, and effects of smoke-free policy implementation.

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Review of HPLC–MS methods for the analysis of nicotine and its active metabolite cotinine in various biological matrices

Jin

Pang

Zhao

et al. 2022

Biomedical Chromatography

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In recent years, tobacco smoking is a risk factor for a series of diseases, including cardiovascular diseases, cerebrovascular diseases, and cancers. Nicotine, the primary component of tobacco smoke, is mainly transformed to its active metabolite cotinine, which is often used as a biomarker for tobacco exposure for its higher blood concentration and longer residence time than nicotine. Various analytical methods have been developed for the determination of nicotine and cotinine in biological matrices. This article reviewed the HPLC-MS based methods for nicotine and/or cotinine analysis in various biological matrices. The sample preparation, mass and chromatographic conditions, and method validation results of these methods have been summarized and analyzed. The sample was mainly pretreated by protein precipitation and/or extraction.Separation was achieved using methanol and/or acetonitrile:water (with or without ammonium acetate) on C18 columns and acetonitrile:water (with formic acid, ammonium acetate/formate) on HILIC columns. Nicotine-d3, nicotine-d4, and cotinine-d3 were commonly used internal standards (ISs). Other non-deuterated ISs such as ritonavir, N-ethylnorcotinine, and milrinone were also used. For both nicotine and cotinine, the calibration range was 0.005-35,000 ng/mL, the matrix effect was 75.96-126.8%, and the recovery was 53-124.5%. The two analytes were stable at room temperature for 1-10 days, at À80 C for up to 6 months, and after three to six freeze-thaw cycles.Comedications did not affect nicotine and cotinine analyses.

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Nicotine levels in silicone wristband samplers worn by children exposed to secondhand smoke and electronic cigarette vapor are highly correlated with child’s urinary cotinine

Cited by 56 publications

References 32 publications

Assessment of Multipollutant Exposures During Pregnancy Using Silicone Wristbands

Assessment of Multipollutant Exposures During Pregnancy Using Silicone Wristbands

Nicotine, Cotinine, and Tobacco-Specific Nitrosamines Measured in Children’s Silicone Wristbands in Relation to Secondhand Smoke and E-cigarette Vapor Exposure

Review of HPLC–MS methods for the analysis of nicotine and its active metabolite cotinine in various biological matrices

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