Nicotinic and muscarinic synaptic transmission in canine intracardiac ganglion cells innervating the sinoatrial node

Xi-Moy, S X; Randall, Walter C.; Wurster, Robert D.

doi:10.1016/0165-1838(93)90365-2

Cited by 30 publications

(12 citation statements)

References 31 publications

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“…5B. Focal application of muscarine (5 M) also evoked a biphasic response in a subpopulation of rat intracardiac neurons similar to that observed in intracardiac neurons of guinea pig (Allen and Burnstock 1990) and dog (Xi-Moy et al 1993). The [Ca 2ϩ ] i response was unchanged in the presence of a Ca 2ϩ -free extracellular solution whereas the inward current was reduced (Ϫ68 Ϯ 16 pA, n ϭ 7) and the outward current amplitude was substantially increased (97 Ϯ 18 pA, n ϭ 7).…”

Section: Ach-evoked [Ca 2ϩ ] I Responses and Membrane Currents In Volmentioning

confidence: 84%

“…Expression of mRNAs encoding for M1-M4 receptor subtypes has been detected in intracardiac neurons in vitro and in situ (Hassall et al 1993: Hoover et al 1994. The G-protein-coupled M2 receptor increases the K ϩ conductance in mammalian intracardiac neurons (Allen and Burnstock 1990;Xi-Moy et al 1993), whereas M4 receptor activation inhibits the voltage-dependent N-and L-type Ca 2ϩ channels . However, the intracellular signaling mechanism(s) by which mAChR activation changes [Ca 2ϩ ] i in intracardiac neurons has not been addressed.…”

Section: Introductionmentioning

confidence: 99%

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Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca²⁺in Rat Intracardiac Ganglion Neurons

Beker

Weber

Fink

et al. 2003

Journal of Neurophysiology

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. Muscarinic and nicotinic ACh receptor activation differentially mobilize Ca 2ϩ in rat intracardiac ganglion neurons. J Neurophysiol 90: 1956-1964, 2003. First published May 21, 2003 10.1152/jn.01079.2002. The origin of intracellular Ca 2ϩ concentration ([Ca 2ϩ ] i ) transients stimulated by nicotinic (nAChR) and muscarinic (mAChR) receptor activation was investigated in fura-2-loaded neonatal rat intracardiac neurons. ACh evoked [Ca 2ϩ ] i increases that were reduced to ϳ60% of control in the presence of either atropine (1 M) or mecamylamine (3 M) and to Ͻ20% in the presence of both antagonists. Removal of external Ca 2ϩ reduced ACh-induced responses to 58% of control, which was unchanged in the presence of mecamylamine but reduced to 5% of control by atropine. The nAChR-induced [Ca 2ϩ ] i response was reduced to 50% by 10 M ryanodine, whereas the mAChRinduced response was unaffected by ryanodine, suggesting that Ca 2ϩ release from ryanodine-sensitive Ca 2ϩ stores may only contribute to the nAChR-induced [Ca 2ϩ ] i responses. Perforated-patch whole cell recording at -60 mV shows that the rise in [Ca 2ϩ ] i is concomitant with slow outward currents on mAChR activation and with rapid inward currents after nAChR activation. In conclusion, different signaling pathways mediate the rise in [Ca 2ϩ ] i and membrane currents evoked by ACh binding to nicotinic and muscarinic receptors in rat intracardiac neurons.

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Section: Ach-evoked [Ca 2ϩ ] I Responses and Membrane Currents In Volmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca²⁺in Rat Intracardiac Ganglion Neurons

Beker

Weber

Fink

et al. 2003

Journal of Neurophysiology

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“…Spontaneous synaptic and action potential discharge was recorded from a population of neurons, as reported previously (Edwards et al 1995;Xi-Moy et al 1993). There are no reports of spontaneous EPSPs, action potential discharge, or pacemaker firing in cultured or dissociated ICG neurons (Adams and Cuevas 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Synaptic activation of the neurons in the cardiac ganglia thus needs to be considered as part of this pathway from the brain to the heart. Several animal models have been used for studying synaptic responses in the mature, adult ICG: guinea pigs (Edwards et al 1995); dogs (Bibevski et al 2000;Xi-Moy et al 1993); pigs (Smith 1999); and rats, adult (Selyanko and Skok 1992) and neonatal (Seabrook et al 1990). Nerve-evoked responses were blocked by hexamethonium and so are mediated by nicotinic acetylcholine receptors (Bibevski et al 2000;Selyanko and Skok 1992).…”

Section: Introductionmentioning

confidence: 99%

Developmental Changes in Electrophysiological Properties and Synaptic Transmission in Rat Intracardiac Ganglion Neurons

Rimmer¹,

Harper²

2006

Journal of Neurophysiology

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Rimmer, Katrina and Alexander A. Harper. Developmental changes in electrophysiological properties and synaptic transmission in rat intracardiac ganglion neurons. J Neurophysiol 95: 3543-3552, 2006. First published April 12, 2006 doi:10.1152/jn.01220.2005. We charted postnatal changes in the intrinsic electrophysiological properties and synaptic responses of rat intrinsic cardiac ganglion (ICG) neurons. We developed a whole-mount ganglion preparation of the excised right atrial ganglion plexus. Using intracellular recordings and nerve stimulation we tested the hypothesis that substantial transformations in the intrinsic electrical characteristics and synaptic transmission accompany postnatal development. Membrane potential (E m ) did not change but time constant () and cell capacitance increased with postnatal development. Accordingly, input resistance (R in ) decreased but specific membrane resistance (R m ) increased postnatally.Comparison of the somatic active membrane properties revealed significant changes in electrical phenotype. All neonatal neurons had somatic action potentials (APs) with small overshoots and small afterhyperpolarizations (AHPs). Adult neurons had somatic APs with large overshoots and large AHP amplitudes. The range of AHP duration was larger in adults than in neonates. The AP characteristics of juvenile neurons resembled those of adults, with the exception of AHP duration, which fell midway between neonate and adult values. Phasic, multiply adapting, and tonic evoked discharge activities were recorded from ICG neurons. Most neurons displayed phasic discharge at each developmental stage. All neurons received excitatory synaptic inputs from the vagus or interganglionic nerve trunk(s), the strength of which did not change significantly with postnatal age. The changes in the electrophysiological properties of the postganglionic neuron suggest that increased complexity of parasympathetic regulation of cardiac function accompanies postnatal development.

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“…A similar activation threshold of inward rectifier is reported in myenteric spike after-hyperpolarization neurones (Galligan et al 1989). In addition, to function as a gain control, IM may be involved in muscarinic synaptic transmission (Brown, 1988;Allen & Burnstock, 1990a;Xi-Moy, Randall & Wurster, 1993). Cholinergic slow excitatory postsynaptic potentials have been recorded in canine intracardiac ganglion neurones (Xi-Moy et at.…”

Section: Properties Of Imentioning

confidence: 99%

Potassium currents in adult rat intracardiac neurones.

Xi-Moy

Dun

1995

The Journal of Physiology

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Nicotinic and muscarinic synaptic transmission in canine intracardiac ganglion cells innervating the sinoatrial node

Cited by 30 publications

References 31 publications

Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca²⁺in Rat Intracardiac Ganglion Neurons

Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca²⁺in Rat Intracardiac Ganglion Neurons

Developmental Changes in Electrophysiological Properties and Synaptic Transmission in Rat Intracardiac Ganglion Neurons

Potassium currents in adult rat intracardiac neurones.

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Nicotinic and muscarinic synaptic transmission in canine intracardiac ganglion cells innervating the sinoatrial node

Cited by 30 publications

References 31 publications

Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca2+in Rat Intracardiac Ganglion Neurons

Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca2+in Rat Intracardiac Ganglion Neurons

Developmental Changes in Electrophysiological Properties and Synaptic Transmission in Rat Intracardiac Ganglion Neurons

Potassium currents in adult rat intracardiac neurones.

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Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca²⁺in Rat Intracardiac Ganglion Neurons

Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca²⁺in Rat Intracardiac Ganglion Neurons