NifH gene analysis of endophytic bacteria of sweet potato under various climatic locations

Abstract

Beyond the nodule symbiosis process, some endophytic bacteria can convert the atmospheric nitrogen to available form for their own and the inhabiting host plants. These bacteria possess a nitrogenase enzyme complex involved in producing ammonia from atmospheric dinitrogen. The nitrogenase reductase subunit of the enzyme complex is governed by the nifH gene and is being used as a suitable marker. In the present study, the nifH gene containing endophytic bacteria had been identified from two sweet potato cultivars grown in different locations. Both the cultureindependent and culture-dependent methods were used to identify the nitrogen-fixing bacteria in sweet potatoes. The nifH sequences closest to Azospirillum, Bradyrhizobium, Herbaspirillum, Klebsiella, Kluyvera, Paenibacillus, Pseudacidovorax, Rhizobium and Sphingomonas were identified in this study. It was supposed that soils and varieties have more effects than climatic factors on nitrogen-fixing bacteria of sweet potato. These nitrogen-fixing bacteria could be utilized to develop biofertilizers helpful for sustainable agriculture.

A review on mechanisms and prospects of endophytic bacteria in biocontrol of plant pathogenic fungi and their plant growth-promoting activities

“…P. polymyxa CR1 primed drought stress tolerance and root growth of soybean and arabidopsis [ 192 ]. It was found that endophytes can transfer and solubilize nutrient elements like phosphorus, nitrogen, potassium, and other micronutrients and make them available to plants [ 193 , 194 ].…”

Identification, Characterization, and Growth-Promoting Effects of Bacterial Endophytes Isolated from Okra (Abelmoschus esculentus L.)

“…Endophytes live inside plants, colonizing the internal tissues of host plants, and can establish a mutualistic association without harming the host plants [23][24][25]. Endophytes promote plant growth in many ways, including by phytohormone production [26,27], siderophores production [28,29], expression of 1-aminocyclopropane-1-carboxylate (ACC) deaminase [30,31], and nitrogen fixation [32,33]. In addition, some endophytes have been reported to be capable of protecting plants by producing antipathogenic substances [34,35], ameliorating disease development [36,37], and inducing resistance to biotic and abiotic stresses [23].…”